GRSF1 (NM_001098477) Human Untagged Clone

CAT#: SC316360

GRSF1 (untagged)-Human G-rich RNA sequence binding factor 1 (GRSF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRSF1
• Synonyms: FLJ13125
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001098477, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGATGTGCTTAACTTTTTTTCAGACTGCAGAATCCGCAACGGTGAGAATGGAATACATTTTCTCC
  TAAACAGAGATGGGAAACGAAGGGGTGATGCCTTAATTGAAATGGAGTCAGAGCAGGATGTGCAGAAAGC
  CTTAGAGAAGCACCGCATGTACATGGGCCAGCGGTATGTGGAAGTATATGAGATAAACAATGAAGATGTG
  GATGCCTTAATGAAGAGCTTGCAGGTCAAATCTTCGCCTGTGGTAAATGATGGTGTGGTTCGTTTGAGAG
  GACTTCCTTATAGTTGCAATGAGAAAGACATTGTAGACTTCTTTGCAGGACTGAATATAGTTGACATTAC
  TTTTGTGATGGACTATAGAGGGAGGCGAAAAACAGGGGAAGCCTATGTGCAATTTGAAGAACCAGAAATG
  GCCAACCAAGCCCTGTTGAAACACAGGGAAGAAATTGGTAATCGATACATCGAGATATTTCCAAGCAGAA
  GGAATGAAGTTCGAACACATGTCGGTTCTTATAAGGGAAAGAAAATCGCATCTTTTCCTACTGCTAAGTA
  TATAACTGAGCCAGAAATGGTCTTTGAAGAACATGAAGTAAATGAGGATATTCAACCCATGACAGCTTTT
  GAAAGTGAGAAGGAAATAGAATTGCCTAAGGAGGTGCCAGAAAAGCTTCCAGAGGCTGCTGATTTTGGAA
  CTACGTCTTCTCTGCATTTTGTCCACATGAGAGGATTACCTTTCCAAGCCAATGCCCAAGACATTATAAA
  CTTTTTTGCTCCACTCAAGCCTGTTAGAATCACCATGGAATACAGCTCCAGTGGGAAGGCCACTGGAGAA
  GCTGATGTGCACTTTGAGACCCATGAGGATGCTGTTGCAGCGATGCTCAAGGATCGGTCCCACGTTCATC
  ATAGGTATATTGAACTGTTCCTGAATTCATGTCCAAAAGGAAAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001098477
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098477.1, NP_001091947.1
• RefSeq Size: 6482 bp
• RefSeq ORF: 957 bp
• Locus ID: 2926
• Cytogenetics: 4q13.3
• Gene Summary: 'The protein encoded by this gene is a cellular protein that binds RNAs containing the G-rich element. The protein is localized in the cytoplasm, and has been shown to stimulate translation of viral mRNAs in vitro. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains an alternate exon in the 5' coding region and uses a downstream start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.