Liver Carboxylesterase 1 (CES1) (NM_001266) Human Untagged Clone

CAT#: SC316626

CES1 (untagged)-Human carboxylesterase 1 (CES1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CES1
• Synonyms: ACAT; CE-1; CEH; CES2; hCE-1; HMSE; HMSE1; PCE-1; REH; SES1; TGH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001266 edited
  ACTGTCGCCCTTCCACGATGTGGCTCCGTGCCTTTATCCTGGCCACTCTCTCTGCTTCCG
  CGGCTTGGGGGCATCCGTCCTCGCCACCTGTGGTGGACACCGTGCATGGCAAAGTGCTGG
  GGAAGTTCGTCAGCTTAGAAGGATTTGCACAGCCTGTGGCCATTTTCCTGGGAATCCCTT
  TTGCCAAGCCGCCTCTTGGACCCCTGAGGTTTACTCCACCGCAGCCTGCAGAACCATGGA
  GCTTTGTGAAGAATGCCACCTCGTACCCTCCTATGTGCACCCAAGATCCCAAGGCGGGGC
  AGTTACTCTCAGAGCTATTTACAAACCGAAAGGAGAACATTCCTCTCAAGCTTTCTGAAG
  ACTGTCTTTACCTCAATATTTACACTCCTGCTGACTTGACCAAGAAAAACAGGCTGCCGG
  TGATGGTGTGGATCCACGGAGGGGGGCTGATGGTGGGTGCGGCATCAACCTATGATGGGC
  TGGCCCTTGCTGCCCATGAAAACGTGGTGGTGGTGACCATTCAATATCGCCTGGGCATCT
  GGGGATTCTTCAGCACAGGGGATGAACACAGCCGGGGGAACTGGGGTCACCTGGACCAGG
  TGGCTGCCCTGCGCTGGGTCCAGGACAACATTGCCAGCTTTGGAGGGAACCCAGGCTCTG
  TGACCATCTTTGGAGAGTCAGCGGGAGGAGAAAGTGTCTCTGTTCTTGTTTTGTCTCCAT
  TGGCCAAGAACCTCTTCCACCGGGCCATTTCTGAGAGTGGCGTGGCCCTCACTTCTGTTC
  TGGTGAAGAAAGGTGATGTCAAGCCCTTGGCTGAGCAAATTGCTATCACTGCTGGGTGCA
  AAACCACCACCTCTGCTGTCATGGTTCACTGCCTGCGACAGAAGACGGAAGAGGAGCTCT
  TGGAGACGACATTGAAAATGAAATTCTTATCTCTGGACTTACAGGGAGACCCCAGAGAGA
  GTCAACCCCTTCTGGGCACTGTGATTGATGGGATGCTGCTGCTGAAAACACCTGAAGAGC
  TTCAAGCTGAAAGGAATTTCCACACTGTCCCCTACATGGTCGGAATTAACAAGCAGGAGT
  TTGGCTGGTTGATTCCAATGCAGTTGATGAGCTATCCACTCTCCGAAGGGCAACTGGACC
  AGAAGACAGCCATGTCACTCCTGTGGAAGTCCTATCCCCTTGTTTGCATTGCTAAGGAAC
  TGATTCCAGAAGCCACTGAGAAATACTTAGGAGGAACAGACGACACTGTCAAAAAGAAAG
  ACCTGTTCCTGGACTTGATAGCAGATGTGATGTTTGGTGTCCCATCTGTGATTGTGGCCC
  GGAACCACAGAGATGCTGGAGCACCCACCTACATGTATGAGTTTCAGTACCGTCCAAGCT
  TCTCATCAGACATGAAACCCAAGACGGTGATAGGAGACCACGGGGATGAGCTCTTCTCCG
  TCTTTGGGGCCCCATTTTTAAAAGAGGGTGCCTCAGAAGAGGAGATCAGACTTAGCAAGA
  TGGTGATGAAATTCTGGGCCAACTTTGCTCGCAATGGAAACCCCAATGGGGAAGGGCTGC
  CCCACTGGCCAGAGTACAACCAGAAGGAAGGGTATCTGCAGATTGGTGCCAACACCCAGG
  CGGCCCAGAAGCTGAAGGACAAAGAAGTAGCTTTCTGGACCAACCTCTTTGCCAAGAAGG
  CAGTGGAGAAGCCACCCCAGACAGAACACATAGAGCTGTGAATGAAGATCCAGCCGGCCT
  TGGGAGCCTGGAGGAGCAAAGACTGGGGTCTTTTGCGAAAGGGATTGCAGGTTCAGAAGG
  CATCTTACCATGGCTGGGGAATTGTCTGGTGGTGGGGGGCAGGGGACAGAGGCCATGAAG
  GAGCAAGTTTTGTATTTGTGACCTCAGCTTTGGGAATAAAGGATCTTTTGAAGGCCAAAA
  AAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001266 unedited
  NAAGAGCGCTGNCANATATTTGTATACGATTCACTATAGGCGGCCGCGCGAAACTGTCGC
  CCTTCCCGATGTGGCTCCGTGCCTTTATCCTGGCCACTCTCTCTGCTTCCGCGGCTTGGG
  GGCATCCGTCCTCGCCACCTGTGGTGGACACCGTGCATGGCAAAGTGCTGGGGAAGTTCG
  TCAGCTTAGAAGGATTTGCACAGCCTGTGGCCATTTTCCTGGGAATCCCTTTTGCCAAGC
  CGCCTCTTGGACCCCTGAGGTTTACTCCACCGCAGCCTGCAGAACCATGGAGCTTTGTGA
  AGAATGCCACCTCGTACCCTCCTATGTGCACCCAAGATCCCAAGGCGGGGCAGTTACTCT
  CAGAGCTATTTACAAACCGAAAGGAGAACATTCCTCTCAAGCTTTCTGAAGACTGTCTTT
  ACCTCAATATTTACACTCCTGCTGACTTGACCAAGAAAAACAGGCTGCCGGTGATGGTGT
  GGATCCACGGAGGGGGGCTGATGGTGGGTGCGGCATCAACCTATGATGGGCTGGCCCTTG
  CTGCCCATGAAAACGTGGTGGTGGTGACCATTCAATATCGCCTGGGCATCTGGGGATTCT
  TCAGCACAGGGGATGAACACAGCCGGGGGAACTGGGGTCACCTGGACCAGGTGGCTGCCC
  TGCGCTGGGTCCAGGACAACATTGCCAGCTTTGGAGGGAACCCAGGCTCTGTGACCATCT
  TTGGAGAGTCAGCGGGAGGAGAAAGTGNCTCTGTTCTTGTTTTGNCTCCATTGGCCAAGA
  ACCTCTTCCACCGGGCCATTTCTGAGAGTGGCGTGGCCCTCACTTCTGNTCTGGTGAAGA
  AAGGTGATGTCAAAGCCTTGGGCTGAGCAATGCTAC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001266 unedited
  GTCTGCCTGCGAGAGAGGAAGAGGAGCTCTCTGGAGACGACATTGAAAATGAAATTCTTA
  TCTCTGGACTTACAGGGAGACCCCAGAGAGAGTCAACCCCTTCTGGGCACTGTGATTGAT
  GGGATGCTGCTGCTGAAAACACCTGAAGAGCTTCAAGCTGAAAGGAATTTCCACACTGTC
  CCCTACATGGTCGGAATTAACAAGCAGGAGTTTGGCTGGTTGATTCCAATGCAGTTGATG
  AGCTATCCACTCTCCGAAGGGCAACTGGACCAGAAGACAGCCATGTCACTCCTGTGGAAG
  TCCTATCCCCTTGTTTGCATTGCTAAGGAACTGATTCCAGAAGCCACTGAGAAATACTTA
  GGAGGAACAGACGACACTGTCAAAAAGAAAGACCTGTTCCTGGACTTGATAGCAGATGTG
  ATGTTTGGTGTCCCATCTGTGATTGTGGCCCGGAACCACAGAGATGCTGGAGCACCCACC
  TACATGTATGAGTTTCAGTACCGTCCAAGCTTCTCATCAGACATGAAACCCAAGACGGTG
  ATAGGAGACCACGGGGATGAGCTCTTCTCCGTCTTTGGGGCCCCATTTTTAAAAGAGGGT
  GCCTCAGAAGAGGAGATCAGACTTAGCAAGATGGTGATGAAATTCTGGGCCAACTTTGCT
  CGCAATGGAAACCCCAATGGGGAAGGGCTGCCCCACTGGCCAGAGTACAACCAGAAAGAA
  AGGGTATCTGCAGATTGGTGCCAACACCCAGGCGGCCCAGAAGCTGAAGACAAAGAAGTA
  GCTTTCTGGACCAACCTCTTTGCCAAGAAGCAGTGGAGAAGCCACCCCCAGACAGACACA
  TAGAGCTGTGAATGAGATCCAGCCCGGCCTGGGAGCCTGAGAGCAAAGACTGGGGTCTTT
  GCGAAAGGATTGCAGTCAGAGCATCTACATGCTGGGATTGTCTGTGGTGGGGGGCAGGGA
  AAGAGCCCATGAGGAGCAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001266
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to have a single amino acid difference from the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001266.4, NP_001257.4
• RefSeq Size: 2021 bp
• RefSeq ORF: 1701 bp
• Locus ID: 1066
• Cytogenetics: 16q12.2
• Domains: COesterase
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes
• Gene Summary: 'This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (3) uses a different in-frame splice site at the end of two separate exons compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter by 2 aa compared to isoform a.