Meprin beta (MEP1B) (NM_005925) Human Untagged Clone

CAT#: SC318181

MEP1B (untagged)-Human meprin A, beta (MEP1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEP1B
• Synonyms: endopeptidase-2; meprin A, beta; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit; PABA peptide hydrolase; PPH beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005925, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATTTATGGAATCTGTCTTGGTTTCTGTTCTTGGATGCTCTTCTCGTGATTTCTGGCTTGGCAACTC
  CAGAAAACTTTGATGTAGATGGCGGAATGGACCAGGACATATTTGATATCAATGAAGGTTTGGGACTGGA
  TCTTTTTGAGGGTGACATCAGACTTGATAGGGCACAAATTAGAAATTCCATCATTGGAGAAAAGTATAGA
  TGGCCTCATACCATTCCATATGTTCTAGAAGATAGCTTGGAAATGAATGCTAAGGGAGTTATCCTCAATG
  CATTTGAACGTTATCGCCTTAAAACATGTATTGACTTTAAGCCTTGGGCTGGAGAAACAAACTATATATC
  AGTGTTCAAGGGCAGTGGCTGCTGGTCTTCAGTAGGAAATAGGCGGGTTGGGAAGCAAGAACTTTCCATC
  GGGGCAAACTGTGACCGAATAGCAACAGTTCAACACGAGTTCCTCCACGCTCTGGGATTCTGGCATGAGC
  AGTCGCGTTCTGACCGGGATGACTATGTCAGGATAATGTGGGACAGAATTCTGTCAGGCAGAGAGCACAA
  TTTTAACACCTATAGTGACGATATATCAGATTCCCTGAATGTTCCCTATGATTACACTTCAGTAATGCAC
  TACAGTAAAACTGCATTCCAAAATGGAACAGAGCCGACAATTGTCACAAGAATCTCAGACTTTGAGGATG
  TGATCGGCCAACGAATGGATTTCAGTGACTCTGATCTCCTAAAGTTGAATCAACTGTATAACTGCTCCTC
  TTCCTTGAGTTTTATGGACTCGTGCAGTTTTGAACTGGAAAATGTGTGTGGCATGATCCAAAGTTCAGGA
  GATAATGCTGACTGGCAACGGGTTTCACAGGTTCCCAGGGGGCCAGAGAGTGATCACTCCAACATGGGCC
  AGTGCCAAGGTTCTGGTTTCTTCATGCATTTCGATAGCAGCTCTGTAAATGTGGGGGCCACAGCAGTGCT
  GGAAAGTAGAACGCTGTACCCTAAAAGAGGATTTCAGTGCCTGCAATTTTACTTATATAACAGTGGCAGT
  GAAAGTGATCAACTGAACATCTATATCAGGGAGTATTCTGCAGACAATGTGGATGGCAATTTAACCCTTG
  TGGAAGAAATAAAAGAAATACCCACTGGGAGCTGGCAACTTTATCATGTAACATTGAAAGTGACCAAGAA
  GTTTAGAGTGGTGTTTGAAGGACGCAAAGGCTCTGGTGCATCACTGGGTGGTCTGTCTATTGATGACATC
  AATCTTTCGGAAACACGGTGCCCTCATCATATCTGGCATATAAGGAATTTCACACAGTTCATTGGCAGCC
  CAAATGGAACTCTGTATAGCCCTCCATTTTACTCTTCTAAAGGTTATGCCTTTCAGATTTACTTAAATCT
  AGCCCATGTGACTAATGCAGGGATATATTTCCACTTGATCTCTGGAGCCAATGATGATCAATTACAGTGG
  CCATGTCCTTGGCAACAAGCCACAATGACACTTTTGGATCAAAATCCTGACATTCGACAGCGTATGTCCA
  ATCAGCGGAGTATAACTACAGACCCATTTATGACCACCGATAATGGAAACTATTTCTGGGACAGGCCTTC
  TAAAGTGGGAACAGTGGCTTTGTTCTCTAATGGAACTCAGTTTAGAAGAGGTGGGGGCTATGGAACCAGT
  GCCTTTATAACCCACGAAAGGCTGAAAAGCAGAGATTTTATAAAAGGAGATGATGTTTATATCCTACTGA
  CAGTGGAAGACATATCTCACCTCAACTCTACACAAATCCAGCTAACACCAGCCCCTAGTGTTCAAGACCT
  CTGCTCAAAAACCACCTGTAAAAATGACGGTGTCTGCACTGTTCGAGATGGCAAAGCTGAGTGCAGGTGC
  CAGTCAGGGGAAGACTGGTGGTACATGGGAGAAAGGTGTGAAAAGAGAGGCTCCACCCGAGACACCATAG
  TCATTGCTGTTTCATCTACTGTTGCTGTGTTTGCCTTGATGCTGATCATCACCCTTGTCAGTGTCTATTG
  CACCAGGAAGAAATATCGTGAAAGGATGAGCTCAAATCGACCAAATTTGACTCCGCAAAATCAGCATGCT
  TTTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005925
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005925.2, NP_005916.2
• RefSeq Size: 2327 bp
• RefSeq ORF: 2106 bp
• Locus ID: 4225
• Cytogenetics: 18q12.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).