TLE4 (NM_007005) Human Untagged Clone

CAT#: SC318250

TLE4 (untagged)-Human transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila) (TLE4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLE4
• Synonyms: BCE-1; BCE1; E(spI); E(spl); ESG; ESG4; Grg-4; GRG4
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007005 edited
  ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACCAGACACCCGGCACCGCATCAGCCT
  GCTCAACCCTTTAAATTTACAATTTCCGAATCCTGTGATCGGATTAAGGAAGAGTTTCAG
  TTTTTACAGGCTCAATACCACAGTCTGAAGCTGGAATGTGAGAAACTCGCCAGTGAGAAG
  ACAGAGATGCAGCGGCATTATGTCATGTATTATGAAATGTCCTATGGGTTGAATATAGAA
  ATGCACAAGCAGGCAGAGATTGTCAAGAGGCTGAATGCTATCTGTGCACAAGTCATTCCT
  TTCCTGTCCCAAGAGCACCAGCAACAAGTGGTGCAGGCTGTGGAACGGGCCAAGCAGGTG
  ACCATGGCAGAACTGAACGCCATCATTGGGCAACAACTCCAGGCCCAGCATTTATCACAT
  GGACATGGTCTCCCCGTACCTCTGACTCCACACCCTTCAGGGCTCCAGCCCCCTGCCATT
  CCACCCATCGGTAGCAGTGCCGGGCTTCTGGCCCTCTCCAGTGCTCTAGGAGGTCAGTCC
  CATCTTCCAATTAAAGATGAGAAGAAGCACCATGACAATGATCACCAAAGAGACAGAGAC
  TCCATCAAGAGCTCTTCAGTATCCCCATCAGCCAGTTTCCGAGGTGCTGAGAAGCACAGA
  AACTCCGCAGACTACTCCTCAGAGAGCAAAAAGCAGAAAACTGAAGAAAAGGAAATTGCA
  GCTCGTTATGACAGCGATGGTGAGAAAAGTGATGACAACTTGGTGGTTGACGTTTCCAAT
  GAGGATCCATCTTCCCCTCGAGGGAGCCCAGCACATTCCCCCAGAGAGAATGGCCTAGAC
  AAGACACGCCTGCTCAAGAAAGATGCCCCGATTAGTCCAGCCTCTATTGCATCTTCCAGC
  AGTACTCCCTCCTCCAAATCCAAAGAACTTAGCCTTAATGAAAAATCTACTACTCCCGTC
  TCAAAGTCCAATACCCCTACTCCACGAACTGATGCGCCCACCCCAGGCAGTAACTCTACT
  CCCGGATTGAGGCCTGTACCTGGAAAACCACCAGGAGTTGACCCTTTGGCCTCAAGCCTA
  AGGACCCCAATGGCAGTACCTTGTCCATATCCAACTCCATTTGGGATTGTGCCCCATGCT
  GGAATGAACGGAGAGCTGACCAGCCCCGGAGCGGCCTACGCTGGGCTCCACAACATCTCC
  CCTCAGATGAGCGCAGCTGCTGCCGCCGCCGCTGCTGCTGCTGCCTATGGGAGATCACCA
  GTGGTGGGATTTGATCCACACCATCACATGCGTGTGCCAGCAATACCTCCAAACCTGACA
  GGCATTCCAGGAGGAAAACCAGCATACTCCTTCCATGTTAGCGCAGATGGTCAGATGCAG
  CCTGTCCCTTTTCCACCCGACGCCCTCATCGGACCTGGAATCCCCCGGCATGCTCGCCAG
  ATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCGGTGACCATCAGCAACCCCACGAGA
  CACGTGTACACGGGTGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGCCACCCAGGCAAT
  AAGAGTCCTGTCTCCCAGCTCGACTGTCTGAACAGGGATAACTACATCCGTTCCTGCAGA
  TTGCTCCCTGATGGTCGCACCCTAATTGTTGGAGGGGAAGCCAGTACTTTGTCCATTTGG
  GACCTGGCGGCTCCAACCCCACGCATCAAGGCAGAGCTGACATCCTCGGCCCCCGCCTGC
  TATGCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAAC
  ATCGCTGTGTGGGATCTGCACAACCAGACCTTGGTGAGGCAATTCCAGGGCCACACAGAT
  GGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACAGGTGGTTTGGAC
  AACACGGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAGCACGACTTCACC
  TCCCAGATCTTTTCTCTGGGCTACTGCCCAACTGGAGAGTGGCTTGCAGTGGGGATGGAG
  AACAGCAATGTGGAAGTTTTGCATGTCACCAAGCCAGACAAATACCAACTACATCTTCAT
  GAGAGCTGTGTGCTGTCGCTCAAGTTTGCCCATTGTGGCAAATGGTTTGTAAGCACTGGA
  AAGGACAACCTTCTGAATGCCTGGAGAACACCTTATGGGGCCAGTATATTCCAGTCCAAA
  GAATCCTCATCGGTGCTTAGCTGTGACATCTCCGTGGACGACAAATACATTGTCACTGGC
  TCTGGGGATAAGAAGGCCACAGTTTATGAAGTTATTTATTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_007005
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007005.3, NP_008936.2
• RefSeq Size: 4749 bp
• RefSeq ORF: 2322 bp
• Locus ID: 7091
• Cytogenetics: 9q21.31
• Protein Families: Transcription Factors
• Gene Summary: ''
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.