REST (NM_005612) Human Untagged Clone

CAT#: SC318496

REST (untagged)-Human RE1-silencing transcription factor (REST), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: REST
• Synonyms: DFNA27; GINGF5; HGF5; NRSF; WT6; XBR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005612 edited
  ACCGAGGAAGGCCGAATACAGTTATGGCCACCCAGGTAATGGGGCAGTCTTCTGGAGGAG
  GAGGGCTGTTTACCAGCAGTGGCAACATTGGAATGGCCCTGCCTAACGACATGTATGACT
  TGCATGACCTTTCCAAAGCTGAACTGGCCGCACCTCAGCTTATTATGCTGGCAAATGTGG
  CCTTAACTGGGGAAGTAAATGGCAGCTGCTGTGATTACCTGGTCGGTGAAGAAAGACAGA
  TGGCAGAACTGATGCCGGTTGGGGATAACAACTTTTCAGATAGTGAAGAAGGAGAAGGAC
  TTGAAGAGTCTGCTGATATAAAAGGTGGACCTCATGGACTGGAAAACATGGAACTGAGAA
  GTTTGGAACTCAGCGTCGTAGAACCTCAGCCTGTATTTGAGGCATCAGGTGCTCCAGATA
  TTTACAGTTCAAATAAAGATCTTCCCCCTGAAACACCTGGAGCGGAGGACAAAGGCAAGA
  GCTCGAAGACCAAACCCTTTCGCTGTAAGCCATGCCAATATGAAGCAGAATCTGAAGAAC
  AGTTTGTGCATCACATCAGAGTTCACAGTGCTAAGAAATTTTTTGTGGAAGAGAGTGCAG
  AGAAGCAGGCAAAAGCCAGGGAATCTGGCTCTTCCACTGCAGAAGAGGGAGATTTCTCCA
  AGGGCCCCATTCGCTGTGACCGCTGCGGCTACAATACTAATCGATATGATCACTATACAG
  CACACCTGAAACACCACACCAGAGCTGGGGATAATGAGCGAGTCTACAAGTGTATCATTT
  GCACATACACAACAGTGAGCGAGTATCACTGGAGGAAACATTTAAGAAACCATTTTCCAA
  GGAAAGTATACACATGTGGAAAATGCAACTATTTTTCAGACAGAAAAAACAATTATGTTC
  AGCATGTTAGAACTCATACAGGAGAACGCCCATATAAATGTGAACTTTGTCCTTACTCAA
  GTTCTCAGAAGACTCATCTAACTAGACATATGCGTACTCATTCAGGTGAGAAGCCATTTA
  AATGTGATCAGTGCAGTTATGTGGCCTCTAATCAACATGAAGTAACCCGCCATGCAAGAC
  AGGTTCACAATGGGCCTAAACCTCTTAATTGCCCACACTGTGATTACAAAACAGCAGATA
  GAAGCAACTTCAAAAAACATGTAGAGCTACATGTGAACCCACGGCAGTTCAATTGCCCTG
  TATGTGACTATGCAGCTTCCAAGAAGTGTAATCTACAGTATCACTTCAAATCTAAGCATC
  CTACTTGTCCTAATAAAACAATGGATGTCTCAAAAGTGAAACTAAAGAAAACCAAAAAAC
  GAGAGGCTGACTTGCCTGATAATATTACCAATGAAAAAACAGAAATAGAACAAACAAAAA
  TAAAAGGGGATGTGGCTGGAAAGAAAAATGAAAAGTCCGTCAAAGCAGAGAAAAGAGATG
  TCTCAAAAGAGAAAAAGCCTTCTAATAATGTGTCAGTGATCCAGGTGACTACCAGAACTC
  GAAAATCAGTAACAGAGGTGAAAGAGATGGATGTGCATACAGGAAGCAATTCAGAAAAAT
  TCAGTAAAACTAAGAAAAGCAAAAGGAAGCTGGAAGTTGACAGCCATTCTTTACATGGTC
  CTGTGAATGATGAGGAATCTTCAACAAAAAAGAAAAAGAAGGTAGAAAGCAAATCCAAAA
  ATAATAGTCAGGAAGTGCCAAAGGGTGACAGCAAAGTGGAGGAGAATAAAAAGCAAAATA
  CTTGCATGAAAAAAAGTACAAAGAAGAAAACTCTGAAAAATAAATCAAGTAAGAAAAGCA
  GTAAGCCTCCTCAGAAGGAACCTGTTGAGAAGGGATCTGCTCAGATGGACCCTCCTCAGA
  TGGGGCCTGCTCCCACAGAGGCGGTTCAGAAGGGGCCCATTCAGGTGGAGCCGCCACCTC
  CCATGGAGCATGCTCAGATGGAGGGTGCCCAGATACGGCCTGCTCCTGACGAGCCTGTTC
  AGATGGAGGTGGTTCAGGAGGGGCCTGCTCAGAAGGAGCTGCTGCCTCCCGTGGAGCCTG
  CTCAGATGGTGGGTGCCCAAATTGTACTTGCTCACATGGAGCTGCCTCCTCCCATGGAGA
  CTGCTCAGACGGAGGTTGCCCAAATGGGGCCTGCTCCCATGGAACCTGCTCAGATGGAGG
  TTGCCCAGGTAGAATCTGCTCCCATGCAGGTGGTCCAGAAGGAGCCTGTTCAGATGGAGC
  TGTCTCCTCCCATGGAGGTGGTCCAGAAGGAGCCTGTTCAGATAGAGCTGTCTCCTCCCA
  TGGAGGTGGTCCAGAAGGAACCTGTTAAGATAGAGCTGTCTCCTCCCATAGAGGTGGTCC
  AGAAGGAGCCTGTTCAGATGGAGTTGTCTCCTCCCATGGGGGTGGTTCAGAAGGAGCCTG
  CTCAGAGGGAGCCACCTCCTCCCAGAGAGCCTCCCCTTCACATGGAGCCAATTTCCAAAA
  AGCCTCCTCTCCGAAAAGATAAAAAGGAAAAGTCTAACATGCAGAGTGAAAGGGCACGGA
  AGGAGCAAGTCCTTATTGAAGTTGGCTTAGTGCCTGTTAAAGATAGCTGGCTTCTAAAGG
  AAAGTGTAAGCACAGAGGATCTCTCACCACCATCACCACCACTGCCAAAGGAAAATTTAA
  GAGAAGAGGCATCAGGAGACCAAAAATTACTCAACACAGGTGAAGGAAATAAAGAAGCCC
  CTCTTCAGAAAGTAGGAGCAGAAGAGGCAGATGAGAGCCTACCTGGTCTTGCTGCTAATA
  TCAACGAATCTACCCATATTTCATCCTCTGGACAAAACTTGAATACGCCAGAGGGTGAAA
  CTTTAAATGGTAAACATCAGACTGACAGTATAGTTTGTGAAATGAAAATGGACACTGATC
  AGAACACAAGAGAGAATCTCACTGGTATAAATTCAACAGTTGAAGAACCAGTTTCACCAA
  TGCTTCCCCCTTCAGCAGTAGAAGAACGTGAAGCAGTGTCCAAAACTGCACTGGCATCAC
  CTCCTGCTACAATGGCAGCAAATGAGTCTCAGGAAATTGATGAAGATGAAGGCATCCACA
  GCCATGAAGGAAGTGACCTAAGTGACAACATGTCAGAGGGTAGTGATGATTCTGGATTGC
  ATGGGGCTCGGCCAGTTCCACAAGAATCTAGCAGAAAAAATGCAAAGGAAGCGTTGGCAG
  TCAAAGCGGCTAAGGGAGATTTTGTTTGTATCTTCTGTGATCGTTCTTTCAGAAAGGGAA
  AAGATTACAGCAAACACCTCAATCGCCATTTGGTTAATGTGTACTATCTTGAAGAAGCAG
  CTCAAGGGCAGGAGTAATGAAACTTTGAACAAGGTTTCAGTTCTTAGTTTGTAAGGTATA
  TTACATTTTATATTCATTTATGATAGCAGACAACCTTTTAAGATTGC
  
• Restriction Sites: Please inquire
• ACCN: NM_005612
• Insert Size: 3400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005612.3, NP_005603.2
• RefSeq Size: 3663 bp
• RefSeq ORF: 3294 bp
• Locus ID: 5978
• Cytogenetics: 4q12
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease
• Gene Summary: 'This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.