alpha Adaptin (AP2A2) (NM_012305) Human Untagged Clone

CAT#: SC319157

AP2A2 (untagged)-Human adaptor-related protein complex 2, alpha 2 subunit (AP2A2)


  "NM_012305" in other vectors (4)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol AP2A2
Synonyms ADTAB; CLAPA2; HIP-9; HIP9; HYPJ
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_012305.2 CTGGGACCCTGAGGCGGCCGTGGTTAGGCGGCTCCCCGGCGGCTCCTCCGCGGCGGTGAC
GGCGACCGCACTCCCCGCTTCCCGCTCCCCGCGCTCCTCCGCCCGGGTCCGCCAGCCGAG
GCCGCTCCCGAGCGTCGGAAGATGCCGGCCGTGTCCAAGGGGGACGGGATGCGGGGCCTG
GCGGTCTTCATCTCGGATATCCGCAACTGTAAAAGTAAAGAAGCAGAAATAAAAAGGATA
AACAAGGAACTGGCAAATATCAGATCAAAATTTAAAGGTGACAAGGCTCTTGATGGCTAT
AGTAAAAAAAAGTACGTCTGCAAGTTGCTCTTCATCTTTCTCCTTGGTCATGACATTGAC
TTTGGACACATGGAGGCTGTGAACCTGCTGAGTTCAAACAGATACACGGAAAAGCAGATC
GGCTACCTTTTCATCTCTGTGTTGGTGAACTCAAACAGTGAGCTGATCCGCCTGATCAAC
AACGCCATCAAGAATGACCTGGCCAGCCGCAACCCCACCTTCATGGGCCTGGCCCTGCAC
TGCATCGCCAGCGTGGGCAGCCGGGAGATGGCCGAGGCCTTCGCCGGGGAGATCCCTAAG
GTCCTCGTAGCCGGAGACACTATGGACAGCGTGAAGCAGAGCGCGGCCCTGTGCTTGCTG
CGCCTGTACAGGACGTCCCCCGATCTTGTCCCCATGGGCGACTGGACATCCCGAGTGGTG
CACCTGCTCAATGACCAGCACTTGGGTGTGGTAACTGCAGCCACAAGTCTGATCACCACT
TTAGCACAGAAGAACCCAGAAGAGTTTAAAACCTCCGTGTCTCTGGCTGTCTCTAGGCTA
AGCAGAATCGTGACGTCTGCATCCACAGATCTCCAGGATTACACTTACTATTTTGTCCCG
GCTCCCTGGCTGTCTGTCAAACTGCTGAGACTGCTGCAGTGCTACCCACCCCCAGACCCT
GCAGTGCGAGGCCGCCTGACTGAGTGCCTGGAGACCATCCTGAACAAAGCCCAAGAACCG
CCCAAGTCGAAGAAGGTCCAGCACTCCAACGCGAAGAATGCCGTGCTCTTCGAGGCCATC
AGCTTAATCATTCACCATGACAGTGAGCCGAACCTGCTCGTCCGTGCCTGCAACCAGTTG
GGCCAGTTTCTGCAGCACCGCGAGACCAACCTGCGCTACCTGGCCCTGGAGAGCATGTGC
ACGCTGGCCAGCTCTGAGTTCTCCCATGAGGCTGTCAAGACGCACATCGAGACGGTCATC
AACGCCCTGAAGACTGAGCGGGACGTGAGCGTGCGGCAGCGGGCCGTGGACCTCCTCTAC
GCCATGTGCGACCGCAGCAACGCCCCACAGATCGTGGCCGAGATGCTGAGCTATCTGGAG
ACAGCTGACTACTCCATCCGAGAAGAGATTGTGCTGAAGGTCGCCATCCTGGCTGAGAAG
TACGCGGTGGACTACACCTGGTATGTGGATACCATCTTGAACTTGATCCGAATTGCTGGT
GATTACGTGAGTGAAGAGGTGTGGTACCGAGTCATTCAGATCGTCATCAACCGGGACGAC
GTGCAGGGCTACGCGGCCAAGACTGTGTTCGAGGCTCTTCAGGCTCCCGCGTGCCACGAG
AACCTGGTCAAAGTGGGCGGCTACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGAGAC
CCGAGATCCAGCCCGCTGATCCAGTTCCACCTGCTGCACTCCAAGTTCCACCTGTGCAGC
GTCCCCACCCGCGCGCTGCTCCTGTCCACCTACATCAAGTTCGTGAACCTCTTCCCGGAG
GTGAAGCCCACCATCCAGGACGTGCTGCGCAGCGACAGCCAGCTCAGGAACGCAGACGTG
GAGCTGCAGCAGCGTGCTGTGGAGTACCTGCGGCTCAGCACCGTGGCCAGCACCGACATT
CTGGCGACCGTGCTGGAGGAGATGCCCCCATTCCCGGAGCGGGAGTCCTCCATCTTGGCA
AAGCTCAAGAAGAAGAAGGGCCCCAGCACGGTGACAGACCTGGAGGACACCAAGCGGGAC
AGGAGTGTGGACGTGAACGGGGGTCCTGAGCCTGCCCCAGCCAGTACCAGCGCCGTGTCT
ACGCCTTCTCCGTCGGCAGACCTGCTGGGTCTCGGGGCTGCCCCCCCTGCCCCCGCGGGC
CCCCCACCCTCCTCCGGCGGCAGCGGGCTGCTCGTGGACGTGTTCTCAGACTCGGCCTCT
GTGGTCGCGCCTCTCGCTCCTGGCTCCGAAGACAACTTTGCCAGGTTTGTTTGTAAAAAC
AATGGTGTGTTGTTTGAAAACCAGCTGCTTCAAATTGGACTTAAGTCTGAATTTCGGCAG
AATTTAGGTCGGATGTTTATCTTTTATGGTAATAAGACCTCCACGCAGTTCCTAAACTTT
ACCCCAACACTAATCTGTTCAGACGACCTTCAGCCTAACCTGAACCTGCAGACCAAGCCC
GTGGACCCGACCGTGGAGGGGGGCGCGCAGGTGCAGCAGGTGGTCAACATAGAGTGCGTG
TCCGACTTCACGGAGGCGCCAGTCCTCAACATTCAGTTCAGGTATGGGGGCACCTTCCAG
AACGTGTCTGTGCAGCTGCCCATCACTCTCAACAAATTCTTCCAGCCGACAGAAATGGCT
TCTCAGGATTTCTTTCAACGTTGGAAGCAGTTGAGCAATCCACAGCAGGAAGTGCAGAAC
ATCTTCAAAGCAAAGCACCCAATGGACACAGAAGTCACCAAAGCCAAGATCATTGGATTT
GGTTCTGCACTTCTTGAAGAAGTTGATCCTAATCCTGCGAATTTCGTGGGAGCTGGAATC
ATCCACACGAAAACCACCCAGATTGGATGCCTGCTGCGCTTGGAGCCGAACCTGCAAGCC
CAGATGTACCGGCTCACGCTGCGCACAAGTAAGGAAGCCGTTTCTCAGAGATTATGTGAA
TTGCTCTCAGCGCAGTTTTAGTCCTGAGGATGGAAGACCAGGCTTGTGTGTCTTGTGTTG
TCTTCGTCTGTGCCGTTTGTCTTCGTGGCCATCCTGCAGATGAGCACCGTGTCCAGTGCC
ACAGCACAAGGCGCCTCCCCGCCCCGCCGCCCCACACCTCTCCCCTTTGGGCTGGACGGG
AACACACGTGTGTGGCTCAGGAGGAAAAGCTCAGCCTGGACTGTGGCAGCCACGGCAGAA
GGTGGATCTTGGGATCAATTTTTATAAAAATCGAGACAGTTCTGTGGTTAAATCTACAAA
TTAAAGGGAAATTAGAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_012305
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012305.2, NP_036437.1
RefSeq Size 4616 bp
RefSeq ORF 2820 bp
Locus ID 161
Cytogenetics 11p15.5
Protein Pathways Endocytosis, Huntington's disease
Gene Summary 'The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016]'
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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