ARF6 (NM_001663) Human Untagged Clone

CAT#: SC319190

ARF6 (untagged)-Human ADP-ribosylation factor 6 (ARF6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARF6
• Synonyms: DKFZp564M0264
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001663.2 CTGGGAGGAGGAGTTGGAGGCCGGAGGGAGCCCGCGCTCGGGGCGGCGGCTGGAGGCAGC
  GCACCGAGTTCCCGCGAGGATCCATGACCTGACGGGGCCCCGGAGCCGCGCTGCCTCTCG
  GGTGTCCTGGGTCGGTGGGGAGCCCAGTGCTCGCAGGCCGGCGGGCGGGCCGGAGGGCTG
  CAGTCTCCCTCGCGGTGAGAGGAAGGCGGAGGAGCGGGAACCGCGGCGGCGCTCGCGCGG
  CGCCTGCGGGGGGAAGGGCAGTTCCGGGCCGGGCCGCGCCTCAGCAGGGCGGCGGCTCCC
  AGCGCAGTCTCAGGGCCCGGGTGGCGGCGGCGACTGGAGAAATCAAGTTGTGCGGTCGGT
  GATGCCCGAGTGAGCGGGGGGCCTGGGCCTCTGCCCTTAGGAGGCAACTCCCACGCAGGC
  CGCAAAGGCGCTCTCGCGGCCGAGAGGCTTCGTTTCGGTTTCGCGGCGGCGGCGGCGTTG
  TTGGCTGAGGGGACCCGGGACACCTGAATGCCCCCGGCCCCGGCTCCTCCGACGCGATGG
  GGAAGGTGCTATCCAAAATCTTCGGGAACAAGGAAATGCGGATCCTCATGTTGGGCCTGG
  ACGCGGCCGGCAAGACAACAATCCTGTACAAGTTGAAGCTGGGCCAGTCGGTGACCACCA
  TTCCCACTGTGGGTTTCAACGTGGAGACGGTGACTTACAAAAATGTCAAGTTCAACGTAT
  GGGATGTGGGCGGCCAGGACAAGATCCGGCCGCTCTGGCGGCATTACTACACTGGGACCC
  AAGGTCTCATCTTCGTAGTGGACTGCGCCGACCGCGACCGCATCGATGAGGCTCGCCAGG
  AGCTGCACCGCATTATCAATGACCGGGAGATGAGGGACGCCATAATCCTCATCTTCGCCA
  ACAAGCAGGACCTGCCCGATGCCATGAAACCCCACGAGATCCAGGAGAAACTGGGCCTGA
  CCCGGATTCGGGACAGGAACTGGTATGTGCAGCCCTCCTGTGCCACCTCAGGGGACGGAC
  TCTATGAGGGGCTCACATGGTTAACCTCTAACTACAAATCTTAATGAGCATTCTCCACCC
  ATCCCCTGGAAGGAGAGAAATCAAAAACCCATTCATAGGATTATCGCCACCATCACCTCT
  TTCAATTGCCACTTTCTCTTCTTTTGAATTTGAACTCTGGAGTTACTGTTCTACAGTTTG
  GCGGGGACGGGGCTTGGGGGTTTTCTCTTTTGTTTGTTTCCCTTTCTTTTTCCTTTTTTT
  TTTTTTTTTTTTTTGTTGGCTTTGCGTTAGGATGCTCTGATCTGACATTTGACATGAACA
  CAAAGTTGCTAGATGCTCTTGTTGACTTCCAGCAGATGGGATGGGGGAAACACAGCAGTT
  CTTGGTAAAGTCCTTTGTAATAATAGTTTGATTTTTTTATTTCGAGAGAATCTTTCATTT
  TCCTATGTATGCTTTTTTCCTTTTTTGCCCAGTTTCCTTATCACTTGCTGTAGATGGCTT
  ATTTTGCATTCATGCAGACTATGTTGCAAGTCTGTTTCATCTAGTAAACTGAAAATTATT
  GCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001663
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001663.2, NP_001654.1
• RefSeq Size: 1806 bp
• RefSeq ORF: 528 bp
• Locus ID: 382
• Cytogenetics: 14q21.3
• Domains: RAB, SAR, ARF, arf
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: 'This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]'