p15 INK4b (CDKN2B) (NM_004936) Human Untagged Clone

CAT#: SC319536

CDKN2B (untagged)-Human cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDKN2B
• Synonyms: CDK4I; INK4B; MTS2; P15; p15INK4b; TP15
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004936.3 GTGAGGACTCCGCGACGCGTCCGCACCCTGCGGCCAGAGCGGCTTTGAGCTCGGCTGCGT
  CCGCGCTAGGCGCTTTTTCCCAGAAGCAATCCAGGCGCGCCCGCTGGTTCTTGAGCGCCA
  GGAAAAGCCCGGAGCTAACGACCGGCCGCTCGGCCACTGCACGGGGCCCCAAGCCGCAGA
  AGGACGACGGGAGGGTAATGAAGCTGAGCCCAGGTCTCCTAGGAAGGAGAGAGTGCGCCG
  GAGCAGCGTGGGAAAGAAGGGAAGAGTGTCGTTAAGTTTACGGCCAACGGTGGATTATCC
  GGGCCGCTGCGCGTCTGGGGGCTGCGGAATGCGCGAGGAGAACAAGGGCATGCCCAGTGG
  GGGCGGCAGCGATGAGGGTCTGGCCAGCGCCGCGGCGCGGGGACTAGTGGAGAAGGTGCG
  ACAGCTCCTGGAAGCCGGCGCGGATCCCAACGGAGTCAACCGTTTCGGGAGGCGCGCGAT
  CCAGGTCATGATGATGGGCAGCGCCCGCGTGGCGGAGCTGCTGCTGCTCCACGGCGCGGA
  GCCCAACTGCGCAGACCCTGCCACTCTCACCCGACCGGTGCATGATGCTGCCCGGGAGGG
  CTTCCTGGACACGCTGGTGGTGCTGCACCGGGCCGGGGCGCGGCTGGACGTGCGCGATGC
  CTGGGGTCGTCTGCCCGTGGACTTGGCCGAGGAGCGGGGCCACCGCGACGTTGCAGGGTA
  CCTGCGCACAGCCACGGGGGACTGACGCCAGGTTCCCCAGCCGCCCACAACGACTTTATT
  TTCTTACCCAATTTCCCACCCCCACCCACCTAATTCGATGAAGGCTGCCAACGGGGAGCG
  GCGGAAAGCCTGTAAGCCTGCAAGCCTGTCTGAGACTCACAGGAAGGAGGAGCCGACCGG
  GAATAACCTTCCATACATTTTTTTCTTTGTCTTATCTGGCCCTCGACACTCACCATGAAG
  CGAAACACAGAGAAGCGGATTTCCAGGGATATTTAGGAGTGTGTGACATTCCAGGGGTCG
  TTTGCTTTTCAGGGTTTTCTGAGGGAAAGTGCATATGAAATCCTTGACTGGACCTGGTGG
  CTACGAATCTTCCGATGGATGAATCTCCCACTCCAGCGCTGAGTGGGAGAAGGCAGTGAT
  TAGCACTTGGGTGACGGCAGTCGATGCGTTCACTCCAATGTCTGCTGAGGAGTTATGGTG
  AACCCACAACTTAGGCCCTAGCGGCAGAAAGGAAAACCTGAAGACTGAGGACAAAGTGGA
  GGAGGGCCGAGGTGGGCTTCAGTAAGTCCCCGGCGGCGCTTTAGTTTGAGCGCATGGCAA
  GTCACATGCGTAAACGACACTCTCTGGAAGCCCTGGAGACCCTCGCCCAACTCCACCAGA
  TAGCAGAGGGGTAAGAGAGGATGTGCAAGCGACGACAGATGCTAAAATCCCTGGATCACG
  ACGCTGCAGAGCACCTTTGCACAGGATGCTGGCCTTTGCTCTTACTACACTGAGGAGAGA
  TTCCCGCGGGTTCCGCAGGCAGACTACACAGGATGAGGTGGTGGAGTGGAGTGAGAGCAA
  TTGTAACGGTTAACTGTAACGTTTTCTTTCACACACACACACACACACACACACACACAC
  ATGCTAGGATGCGGAAATCCCCTTATGACTTGCTACTTTTTGATTTTGTGATATTTTGTA
  CTTTTTAGTTGTTCAGCAACTGTCTTATTTAATGGGGAGATTTTAAGTAACATAACTAGT
  GGCTCTCAGTTAAAATGTGAGGAAGAACTACAGCTCTTAAATGTAGCAATGGCACTGTTG
  CAAACTCAGTGCAAACGCCTAGATTGCTTTCTTCTTAACCTATTTATTTCTTTGTTAAAT
  TTTTCTGATTGTTTCCTTTATAGAGTGTCTCAGGGTGCAGAGGTCAGACTAAGAAATATT
  CCAAATGTCTTTTAGAAGATAGATGCACTTATGCAGTAAATTATCTTGGGATAGTTCCCA
  AAAGATTGCTGAAAAAGTAGATTGAGTATAAAAACTTGAAAATATATGATGGCTCGTGGG
  ATGTCCTACTATCACTGAACAAACTAAAGGTGCACTGCTTTGGGATTTAATTTCCAGGGT
  TGCTTGATCATTATATCATTGGAACAACTGATACTTCACTACTTTAATAAAGAATTAACA
  GAGATTGAACTCCAAGAGGTGGGTAATTTGGTTTAAAAATACATGTTCATGGGTTTACCA
  CTAACTCCTGAGAAATGTTAAAGGTTCACAGGGGTTCCCTTCTCTCAATGTTTGTAATAA
  TTGCTCATAAGCAATACCAGCAATTCATAAAAACTGCTTACTTATGCCATAGAAAATTAA
  ACACAAAGTGTATACATGTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004936
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004936.3, NP_004927.2
• RefSeq Size: 3878 bp
• RefSeq ORF: 417 bp
• Locus ID: 1030
• Cytogenetics: 9p21.3
• Domains: ANK
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway
• Gene Summary: 'This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).