MAFG (NM_032711) Human Untagged Clone

CAT#: SC319644

MAFG (untagged)-Human v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) (MAFG), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAFG
• Synonyms: hMAF
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032711.2 CCGCGTCTTATTCCTTCTAATCTCTCCCCCGCCCCTTGTCTTGCGCCTGCCTTCCTAACA
  GCCTTCAGTTCCCCTTCTCCCGTCAGACCTTCCTGACCTCCCACCTGGTTCTCTAGGGGC
  AGGGTGGACAGGAAGCAGCTCAGCCCAGCCTGGGAGAGGCCAAGGGCTGCCTCCTATCAG
  AGAGCGCCTGCTCGCTGTGCCCCCGGGTTATGACGACCCCCAATAAAGGAAACAAGGCCT
  TGAAGGTGAAGCGGGAGCCGGGTGAGAATGGCACCAGCCTGACGGATGAGGAGCTGGTGA
  CCATGTCGGTGCGGGAGCTGAACCAGCACCTGCGGGGCCTGTCCAAGGAGGAGATCGTCC
  AGCTGAAGCAGCGCCGGCGCACGCTCAAGAACCGCGGCTACGCTGCCAGCTGCCGCGTGA
  AGCGGGTGACGCAGAAGGAGGAGCTGGAGAAGCAGAAGGCGGAGCTGCAGCAGGAGGTGG
  AGAAGCTGGCCTCAGAGAACGCCAGCATGAAGCTGGAGCTCGACGCGCTGCGCTCCAAGT
  ACGAGGCGCTGCAGACCTTCGCCCGGACGGTGGCCCGCAGCCCCGTGGCGCCAGCCCGGG
  GCCCCCTTGCCGCCGGCCTGGGGCCCCTCGTCCCAGGCAAGGTGGCCGCCACCAGCGTCA
  TCACAATAGTAAAGTCCAAGACGGATGCCCGATCGTAGGGACGCGCGTCTGCCCAGGCGG
  GTCTTTGCGGGGCCACTAGGCACATGGCGAATTTGGCTGCCCTGTCCCTCTGTTTCCTTC
  TCTTCTCTTTCCTCCCTCTCTTCCCCACCCTTCTCTCTTCCCTGCAAAGCACAACCTGTA
  CCCCAGGGGCGCCGGGCTGAGCCCCTTTGATCTCGTCATTGTCGTCGTGTGTTTTGTATG
  TTGGGATTGGTCAGTTCGGCGGTGACGTGGGGTCGCCCCAACCCCTTTTGTACCAGGGCC
  ATGCAGGCTTGGAGTCCAGAGTTGGTGCTGTGGGAACGGACTAGAGAGAGTTGCGGGAGA
  GAGAAGGAGCAGGCACGCTGGGCCTCGCGTGTCCCCGAGCAGTGAGGGTCCCAGTGTTCC
  CTCCACTCCCGAGTGGCCACAGGCTCGCGGGCTGGGAAGGATTCACTCTCTTTAGCCCCA
  GGGGAGCAGCTCAGCTTAGCCCAGCATGAAGAGATGGGCTCTGCTCTGAGAGTAGGGCGG
  GCTTGAAGGCCCTGATGGGTGGACCACCAGCCTGGGCGCAGTGGTGCTGGGGGCGTGCAG
  CTGGGCCCAGGGGCTGTGCACTCAGGCCTGACCCGTTGCACTGAACAAGACCAAATCGCT
  GGTTGTGCGCTTAACGTGAGGGTGGGTCCAGTGTGCCCTGCGATGGGTCCCGTGTCACTG
  TTTACATGACCTATTTGTGTGGTTATATAGCCCTTTATTTAAAAGAGAGAAGTTCCTTTT
  ACAAAGTTATTAAATTAATTATATGTTTAAAAGTTAAAGAAAAAAGAGCTGCAGAGTATT
  TATAAAACTGTCTTTTAGAAAAAAAACAAGCAAGAAGACCATTTGACCATATGAATGGAA
  AAGGGAAGAAAGTATAATAGAAACTTTGCTAGTTAAAAAAAAAAGAAAAAAAAAAAAAAA
  AAAA
• Restriction Sites: Please inquire
• ACCN: NM_032711
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032711.2, NP_116100.2
• RefSeq Size: 5038 bp
• RefSeq ORF: 489 bp
• Locus ID: 4097
• Cytogenetics: 17q25.3
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.