RBPJK (RBPJ) (NM_203283) Human Untagged Clone

CAT#: SC319844

RBPJ (untagged)-Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RBPJ
• Synonyms: AOS3; CBF1; csl; IGKJRB; IGKJRB1; KBF2; RBP-J; RBPJK; RBPSUH; SUH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_203283.1 ATTGGGTACATGCGCAGTGCCGCTCGCGCGGGCCGCGCCAATCCTGCAGCGCCTTCAACA
  GGTTTCGGGCGGCGAATTCCAGTTCTCCGGGTTTTGGGGCTCCCTGCGGGAGGCAGTGCT
  GGATCTGGGAATCTCTAGGAAAGGAAAGAGCAAAGGAGGGGAAAGATGGGGGGCTGCAGG
  AAATTTGGTGAGCGGCCTCCACCTAAACGACTTACTAGGGAAGCTATGCGAAATTATTTA
  AAAGAGCGAGGGGATCAAACAGTACTTATTCTTCATGCAAAAGTTGCACAGAAGTCATAT
  GGAAATGAAAAAAGGTTTTTTTGCCCACCTCCTTGTGTATATCTTATGGGCAGCGGATGG
  AAGAAAAAAAAAGAACAAATGGAACGCGATGGTTGTTCTGAACAAGAGTCTCAACCGTGT
  GCATTTATTGGGATAGGAAATAGTGACCAAGAAATGCAGCAGCTAAACTTGGAAGGAAAG
  AACTATTGCACAGCCAAAACATTGTATATATCTGACTCAGACAAGCGAAAGCACTTCATG
  TTGTCTGTAAAGATGTTCTATGGCAACAGTGATGACATTGGTGTGTTCCTCAGCAAGCGG
  ATAAAAGTCATCTCCAAACCTTCCAAAAAGAAGCAGTCATTGAAAAATGCTGACTTATGC
  ATTGCCTCAGGAACAAAGGTGGCTCTGTTTAATCGACTACGATCCCAGACAGTTAGTACC
  AGATACTTGCATGTAGAAGGAGGTAATTTTCATGCCAGTTCACAGCAGTGGGGAGCCTTT
  TTTATTCATCTCTTGGATGATGATGAATCAGAAGGAGAAGAATTCACAGTCCGAGATGGC
  TACATCCATTATGGACAAACAGTCAAACTTGTGTGCTCAGTTACTGGCATGGCACTCCCA
  AGATTGATAATTAGGAAAGTTGATAAGCAGACCGCATTATTGGATGCAGATGATCCTGTG
  TCACAACTCCATAAATGTGCATTTTACCTTAAGGATACAGAAAGAATGTATTTGTGCCTT
  TCTCAAGAAAGAATAATTCAATTTCAGGCCACTCCATGTCCAAAAGAACCAAATAAAGAG
  ATGATAAATGATGGCGCTTCCTGGACAATCATTAGCACAGATAAGGCAGAGTATACATTT
  TATGAGGGAATGGGCCCTGTCCTTGCCCCAGTCACTCCTGTGCCTGTGGTAGAGAGCCTT
  CAGTTGAATGGCGGTGGGGACGTAGCAATGCTTGAACTTACAGGACAGAATTTCACTCCA
  AATTTACGAGTGTGGTTTGGGGATGTAGAAGCTGAAACTATGTACAGGTGTGGAGAGAGT
  ATGCTCTGTGTCGTCCCAGACATTTCTGCATTCCGAGAAGGTTGGAGATGGGTCCGGCAA
  CCAGTCCAGGTTCCAGTAACTTTGGTCCGAAATGATGGAATCATTTATTCCACCAGCCTT
  ACCTTTACCTACACACCAGAACCAGGGCCGCGGCCACATTGCAGTGCAGCAGGAGCAATC
  CTTCGAGCCAATTCAAGCCAGGTGCCCCCTAACGAATCAAACACAAACAGCGAGGGAAGT
  TACACAAACGCCAGCACAAATTCAACCAGTGTCACATCATCTACAGCCACAGTGGTATCC
  TAACTACCGTCTTTTTGCTAGGACTTAAACTGACTTGAGTGTGGCAAAAAGTTAACAAAA
  AAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_203283
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203283.1, NP_976028.1
• RefSeq Size: 2272 bp
• RefSeq ORF: 1458 bp
• Locus ID: 3516
• Cytogenetics: 4p15.2
• Protein Families: Transcription Factors
• Protein Pathways: Notch signaling pathway
• Gene Summary: 'The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]'
  Transcript Variant: This variant (3) lacks an alternate 5' exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.