DDX50 (NM_024045) Human Untagged Clone

CAT#: SC320213

DDX50 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 50 (DDX50)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX50
• Synonyms: GU2; GUB; mcdrh; RH-II/GuB
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024045.1 GCATGGCCAAGGCCGGGCGGCCGCCTTGCCCCCGCTTCCTTTCACGCTGTCGCTGCCCGT
  AGGTGGTTGTGGCCACTGTGCCCGGAGGGAGGCGGCGGTGGCCAGTAATGCCTGGGAAAC
  TCCTCTGGGGGGACATTATGGAGCTGGAAGCACCCTTGGAGGAGTCCGAGAGCCAGAAGA
  AGGAGAGGCAAAAGAGTGACAGAAGGAAGTCAAGGCACCATTATGACTCGGATGAGAAAT
  CAGAAACAAGAGAAAATGGTGTTACAGATGACCTGGATGCTCCCAAGGCCAAAAAATCTA
  AAATGAAAGAGAAGCTAAATGGAGACACTGAAGAAGGATTTAATAGACTTTCAGATGAAT
  TCTCCAAATCTCATAAGTCAAGAAGAAAAGATCTACCAAATGGAGATATAGATGAATATG
  AAAAAAAATCAAAGCGAGTATCATCTTTAGATACTTCTACTCATAAATCAAGTGATAATA
  AACTAGAGGAGACCTTAACACGTGAACAGAAAGAAGGAGCCTTCTCCAATTTTCCTATTT
  CTGAAGAGACTATAAAGCTTCTGAAAGGTCGAGGGGTAACATATCTCTTTCCTATTCAAG
  TTAAGACCTTTGGTCCTGTATATGAAGGAAAAGATTTAATAGCTCAAGCACGGACAGGAA
  CAGGAAAGACATTCTCTTTTGCCATCCCCTTAATTGAAAGACTCCAAAGAAATCAAGAAA
  CAATTAAAAAAAGCCGCTCACCAAAGGTACTTGTTTTGGCTCCAACAAGGGAACTGGCAA
  ACCAAGTAGCCAAAGACTTCAAAGATATAACTAGGAAACTCAGCGTGGCGTGTTTTTATG
  GTGGAACATCATATCAAAGCCAAATTAATCATATTCGAAATGGTATTGACATCTTGGTTG
  GAACACCTGGTCGTATCAAAGACCATCTGCAGAGTGGCCGATTGGATCTTTCTAAACTGC
  GACATGTTGTGCTTGATGAAGTGGATCAGATGTTAGATTTAGGTTTCGCTGAACAAGTTG
  AAGATATTATTCATGAATCCTACAAAACTGATTCTGAAGACAATCCTCAGACTTTACTTT
  TTTCTGCAACTTGCCCACAGTGGGTATACAAAGTTGCAAAAAAATACATGAAATCCAGAT
  ATGAACAGGTTGACCTTGTTGGAAAAATGACTCAAAAGGCTGCAACTACTGTGGAACATT
  TGGCCATCCAGTGTCATTGGTCTCAGAGGCCAGCAGTTATTGGAGATGTCCTTCAAGTCT
  ACAGTGGGTCTGAAGGGAGGGCTATTATTTTCTGTGAGACCAAGAAGAATGTAACTGAAA
  TGGCCATGAATCCACACATAAAACAGAATGCCCAGTGTTTACATGGGGACATTGCACAGT
  CACAAAGAGAAATTACACTAAAAGGCTTCAGAGAAGGTAGTTTTAAAGTTTTGGTGGCAA
  CCAATGTGGCTGCCCGTGGTTTGGACATTCCTGAAGTTGACCTGGTGATTCAAAGTTCTC
  CTCCTCAGGATGTTGAGTCCTATATCCATCGCTCTGGACGCACAGGTAGAGCTGGACGGA
  CAGGGATTTGTATATGTTTTTATCAACCAAGAGAAAGAGGTCAACTAAGATATGTGGAAC
  AAAAAGCAGGAATTACTTTTAAACGTGTAGGTGTTCCTTCTACAATGGATTTAGTTAAAT
  CTAAAAGCATGGATGCCATCAGGTCTCTGGCTTCCGTTTCTTATGCTGCTGTTGATTTTT
  TCCGACCATCAGCTCAGAGACTGATAGAAGAGAAAGGTGCAGTGGATGCATTGGCTGCAG
  CTTTAGCCCACATTTCTGGTGCATCAAGCTTTGAACCACGATCTTTGATCACCTCTGATA
  AGGGGTTTGTGACCATGACTCTGGAAAGCCTAGAGGAAATACAGGATGTCAGCTGTGCTT
  GGAAAGAACTTAACAGAAAGCTGAGTAGTAATGCAGTGTCTCAGATTACCAGAATGTGCC
  TCCTGAAAGGAAATATGGGTGTTTGCTTTGATGTTCCTACAACTGAGTCAGAAAGGTTAC
  AGGCAGAGTGGCATGATTCCGACTGGATACTCTCAGTGCCAGCCAAATTACCTGAAATTG
  AAGAATATTATGATGGAAACACATCTTCTAATTCCAGACAGAGGAGTGGCTGGTCAAGTG
  GTCGATCAGGCCGGTCAGGCCGGTCAGGTGGTCGATCTGGCGGCCGGTCAGGTAGACAGA
  GTCGACAAGGAAGTCGCTCAGGAAGTCGACAAGATGGTAGAAGACGAAGTGGGAATAGAA
  ATCGATCAAGAAGTGGGGGCCACAAACGGAGTTTTGACTGAGTATTTGATAGTTAATCTA
  CCAGTGTGAGCTTGCCTATTTCTGCCTAATCATGTACATTATCCACCAAAAATTAGGTCA
  TCATAGTTGAGGTATGTGTCTGCTATTTGCAAAGAAGTTGGTCGTATTTTTTTAAAAAGT
  ATTTCACAAGAATGGAACAAATCTACTTATCCAGTTATACCTTTGAATAAAAAAACCTCC
  TTTTATTGTCTCTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024045.1 unedited
  CTAGTGTGAGCTATCTGATGCTTCCTTATGGACCGTAATTTTTAACTCGACTGGATCCGG
  TACCGAGATATCCGCCGCCGCGGACCGACGCGTTAAGCGGCCGCGAATTCAGATCCACAA
  GTTTGTACAAAAAAGCAGGCTTGTAAAACGACGGCCAGTAACTATAACGGTCCTAAGGTA
  GCGAGGCCTGGGTGGCGAATTCGGCACGAGGGCATGGCCAAGGCCGGGCGGCCGCCTTGC
  CCCCGCTTCCTTTCACGCTGTCGCTGCCCGTAGGTGGTTGTGGCCACTGTGCCCGGAGGG
  AGGCGGCGGTGGCCAGTAATGCCTGGGAAACTCCTCTGGGGGGACATTATGTAGCTGGAA
  GCACCCTTGGAGGAGTCCGAGAGCCAGAATAAGGAGAGGCAAAAGAGTGACAGAAGGAAG
  TCAAGGCACCATTATGACTCGGATGAGAAATCAGAACCAAGAGAAAAATGGTGTTACAGA
  TGACCTGGATGCTCCCCAAGGCCCAAAAAAATCTAAAAATGAAAGAACAGCTAAATGGAG
  ACACTGAAAAAAGGATTTTAATAGACTTTTCAGATGAAATTCTCCCAAATCCCATAAGTC
  AAGAAGAAAAGAATCTACCAATGGGAGATATAGATGAAATATGAAAAAAAAATCAAAGCG
  AGGTATCATCTTTAAAATACTTCTACTCATAAATTCAGGTGAATAATTAGACCTAAAGGA
  GACCTTTACACCGTGAACCGAAAAGAAGGAACCCTTCTCCAAATTTTCTAATTTCTAAAA
  AAACTTATAAGCTTCTGAAAGGCGAGGGGGTACACTAATCCCTTTTCCTATTCCAGTTTA
  AACCCTTCGGTCCCTGGTTTTGAAGGAAAAGTATTTATATGCTCTAGCCCCGGGCCCGGG
  AACCACGGAAGAACACTTTCTCTTTTGGCCATCCCCCCTTAATTATGAAAAGAAAT
• Restriction Sites: Please inquire
• ACCN: NM_024045
• ORF Size: 2214 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_024045.1, NP_076950.1
• RefSeq Size: 2575
• RefSeq ORF: 2214
• Locus ID: 79009
• Domains: DEAD, helicase_C
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. [provided by RefSeq, Jul 2008]