CYB5R3 (NM_000398) Human Untagged Clone

CAT#: SC321426

CYB5R3 (untagged)-Human cytochrome b5 reductase 3 (CYB5R3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYB5R3
• Synonyms: B5R; DIA1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000398.4 GACAGAGCGAGCGCGGCGCGGGGCCACCATGGGGGCCCAGCTCAGCACGTTGGGCCATAT
  GGTGCTCTTCCCAGTCTGGTTCCTGTACAGTCTGCTCATGAAGCTGTTCCAGCGCTCCAC
  GCCAGCCATCACCCTCGAGAGCCCGGACATCAAGTACCCGCTGCGGCTCATCGACCGGGA
  GATCATCAGCCATGACACCCGGCGCTTCCGCTTTGCCCTGCCGTCACCCCAGCACATCCT
  GGGCCTCCCTGTCGGCCAGCACATCTACCTCTCGGCTCGAATTGATGGAAACCTGGTCGT
  CCGGCCCTATACACCCATCTCCAGCGATGATGACAAGGGCTTCGTGGACCTGGTCATCAA
  GGTTTACTTCAAGGACACCCATCCCAAGTTTCCCGCTGGAGGGAAGATGTCTCAGTACCT
  GGAGAGCATGCAGATTGGAGACACCATTGAGTTCCGGGGCCCCAGTGGGCTGCTGGTCTA
  CCAGGGCAAAGGGAAGTTCGCCATCCGACCTGACAAAAAGTCCAACCCTATCATCAGGAC
  AGTGAAGTCTGTGGGCATGATCGCGGGAGGGACAGGCATCACCCCGATGCTGCAGGTGAT
  CCGCGCCATCATGAAGGACCCTGATGACCACACTGTGTGCCACCTGCTCTTTGCCAACCA
  GACCGAGAAGGACATCCTGCTGCGACCTGAGCTGGAGGAACTCAGGAACAAACATTCTGC
  ACGCTTCAAGCTCTGGTACACGCTGGACAGAGCCCCTGAAGCCTGGGACTACGGCCAGGG
  CTTCGTGAATGAGGAGATGATCCGGGACCACCTTCCACCCCCAGAGGAGGAGCCGCTGGT
  GCTGATGTGTGGCCCCCCACCCATGATCCAGTACGCCTGCCTTCCCAACCTGGACCACGT
  GGGCCACCCCACGGAGCGCTGCTTCGTCTTCTGAGGGCCGGGCACGGTCACACGGCCACC
  CGCCCCGCGCACCCCACGCCCTGTTCACGCTCACCCAGTCACCTCCCCACATCGCACACT
  GGGGCCCCGGGTTCAGCCTGGCCTGCCCGTGCCCTGGTGAATCACCTGGCTGAGCAGTTC
  CCCTGGAGCCCCTTCGGGAGCAGGGCTGTGTCCCAGATGGGCCACGGCTGAGCCTTCAGA
  GTACGTCCTGCCTGGCACTTACTGGTCCTTACCAGAGACGCCCAGCCCCATCCCTGTCCT
  CATGACCCCTCGTCCACCCCCCACACACACTATAAGGCTGAGGGCTGCCAGCAGCCCCGT
  CTGCCCACCATTCCCGGCCGTGGACCATAGTCGGGATGTCAGCAGACACACATGGGCAGC
  CCAAAGCTGCAGGTGCCAGGGCCCACCCCAGCCTCGCCTGTCACCCCCACTCCCGCCTCA
  GGGCCAGGCCCAGGCCTCACCACCTGACGCTGCATGAGACATTGACACCAGAAAGCCCTC
  TTGGGGGCACTGCTCCCTACCCCAGGGCCCTGGCCAGCCGGGAGCTTGGCTCTCCTCTGG
  CTAGAGTGGGAAGAGGGGGCTGGCCATGGGGCCCTCCCAGAACCTCAGCATTTCCTTCCA
  GCCCATCCAAACACTGAGGCAGCCTTGGGGAACCCCGAGCTGGGGGGTTGGCAGCCCACT
  GCACCGCCTCAGGGTTTTGGGGTCCTGGGCTGGGGCCACCATCCCTGATGGCAGAACTCC
  CACAACCACATGTATTTATTCCTCTGTCCTAAACCGTCCCCTCCTTCCCTCACCCCCAGC
  ACAGGGGGATTCTGAGCAGTGCCTCTTGTCTGAGGGACATATCAGTGACCTCGACGTTGC
  CTTTAGACTACAGTTGTGTTAGCCTCTTGCGTATTGGCTTTTTCAGAGTCATTTATGAGC
  AGAAAAAAAAAAAGTAAAACTTTGCTAATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AA
• Restriction Sites: Please inquire
• ACCN: NM_000398
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000398.4, NP_000389.1
• RefSeq Size: 1978 bp
• RefSeq ORF: 906 bp
• Locus ID: 1727
• Cytogenetics: 22q13.2
• Domains: NAD_binding_1, FAD_binding_6
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism
• Gene Summary: 'This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (1) encodes the membrane-bound form (isoform 1) of the enzyme. This isoform (1) is referred to in the literature as isoform m. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.