Oct-1 (POU2F1) (NM_002697) Human Untagged Clone

CAT#: SC321473

POU2F1 (untagged)-Human POU class 2 homeobox 1 (POU2F1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POU2F1
• Synonyms: oct-1B; OCT1; OTF1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002697 edited
  CGGAGGAGCAGCGAGTCAAGATGAGAGTTCAGCCGCGGCGGCAGCAGCAGCAGACTGGAA
  AAGTAAGAAGAGCTTTCCTGCCTTTTTAATTACCAAACTACTCTCAGTTTTCAATGAATC
  AGTTCAAAGAAAGAATGCAGTCTTTCTATACCTGACTCAAGAATGAACAATCCGTCAGAA
  ACCAGTAAACCATCTATGGAGAGTGGAGATGGCAACACAGGCACACAAACCAATGGTCTG
  GACTTTCAGAAGCAGCCTGTGCCTGTAGGAGGAGCAATCTCAACAGCCCAGGCGCAGGCT
  TTCCTTGGACATCTCCATCAGGTCCAACTCGCTGGAACAAGTTTACAGGCTGCTGCTCAG
  TCTTTAAATGTACAGTCTAAATCTAATGAAGAATCGGGGGATTCGCAGCAGCCAAGCCAG
  CCTTCCCAGCAGCCTTCAGTGCAGGCAGCCATTCCCCAGACCCAGCTTATGCTAGCTGGA
  GGACAGATAACTGGGCTTACTTTGACGCCTGCCCAGCAACAGTTACTACTCCAGCAGGCA
  CAGGCACAGGCACAGCTGCTGGCTGCTGCAGTGCAGCAGCACTCCGCCAGCCAGCAGCAC
  AGTGCTGCTGGAGCCACCATCTCCGCCTCTGCTGCCACGCCCATGACGCAGATCCCCCTG
  TCTCAGCCCATACAGATCGCACAGGATCTTCAACAACTGCAACAGCTTCAACAGCAGAAT
  CTCAACCTGCAACAGTTTGTGTTGGTGCATCCAACCACCAATTTGCAGCCAGCGCAGTTT
  ATCATCTCACAGACGCCCCAGGGCCAGCAGGGTCTCCTGCAAGCGCAAAATCTTCTAACG
  CAACTACCTCAGCAAAGCCAAGCCAACCTCCTACAGTCGCAGCCAAGCATCACCCTCACC
  TCCCAGCCAGCAACCCCAACACGCACAATAGCAGCAACCCCAATTCAGACACTTCCACAG
  AGCCAGTCAACACCAAAGCGAATTGATACTCCCAGCTTGGAGGAGCCCAGTGACCTTGAG
  GAGCTTGAGCAGTTTGCCAAGACCTTCAAACAAAGACGAATCAAACTTGGATTCACTCAG
  GGTGATGTTGGGCTCGCTATGGGGAAACTATATGGAAATGACTTCAGCCAAACTACCATC
  TCTCGATTTGAAGCCTTGAACCTCAGCTTTAAGAACATGTGCAAGTTGAAGCCACTTTTA
  GAGAAGTGGCTAAATGATGCAGAGAACCTCTCATCTGATTCGTCCCTCTCCAGCCCAAGT
  GCCCTGAATTCTCCAGGAATTGAGGGCTTGAGCCGTAGGAGGAAGAAACGCACCAGCATA
  GAGACCAACATCCGTGTGGCCTTAGAGAAGAGTTTCTTGGAGAATCAAAAGCCTACCTCG
  GAAGAGATCACTATGATTGCTGATCAGCTCAATATGGAAAAAGAGGTGATTCGTGTTTGG
  TTCTGTAACCGCCGCCAGAAAGAAAAAAGAATCAACCCACCAAGCAGTGGTGGGACCAGC
  AGCTCACCTATTAAAGCAATTTTCCCCAGCCCAACTTCACTGGTGGCGACCACACCAAGC
  CTTGTGACTAGCAGTGCAGCAACTACCCTCACAGTCAGCCCTGTCCTCCCTCTGACCAGT
  GCTGCTGTGACGAATCTTTCAGTTACAGGCACTTCAGACACCACCTCCAACAACACAGCA
  ACCGTGATTTCCACAGCGCCTCCAGCTTCCTCAGCAGTCACGTCCCCCTCTCTGAGTCCC
  TCCCCTTCTGCCTCAGCCTCCACCTCCGAGGCATCCAGTGCCAGTGAGACCAGCACAACA
  CAGACCACCTCCACTCCTTTGTCCTCCCCTCTTGGGACCAGCCAGGTGATGGTGACAGCA
  TCAGGTTTGCAAACAGCAGCAGCTGCTGCCCTTCAAGGAGCTGCACAGTTGCCAGCAAAT
  GCCAGTCTTGCTGCCATGGCAGCTGCTGCAGGACTAAACCCAAGCCTGATGGCACCCTCA
  CAGTTTGCGGCTGGAGGTGCCTTACTCAGTCTGAATCCAGGGACCCTGAGCGGTGCTCTC
  AGCCCAGCTCTAATGAGCAACAGTACACTGGCAACTATTCAAGCTCTTGCTTCTGGTGGC
  TCTCTTCCAATAACATCACTTGATGCAACTGGGAACCTGGTATTTGCCAATGCGGGAGGA
  GCCCCCAACATCGTGACTGCCCCTCTGTTCCTGAACCCTCAGAACCTCTCTCTGCTCACC
  AGCAACCCTGTTAGCTTGGTCTCTGCCGCCGCAGCATCTGCAGGGAACTCTGCACCTGTA
  GCCAGCCTTCACGCCACCTCCACCTCTGCTGAGTCCATCCAGAACTCTCTCTTCACAGTG
  GCCTCTGCCAGCGGGGCTGCGTCCACCACCACCACCGCCTCCAAGGCACAGTGAGCTGGG
  CAGAGCTGGGCTGCCAGAAGCCTTTTTCACTCTGCAGTGTGATTGGACTGCCAGCCAGGT
  TAATAAACTGAAAAATGTGATTGGCTTCCTCTCGCCGTGTTGTGAGGGCAAAGGAGAGAA
  GGGAGAAAAAAAAAAAAAAACCACACACACCCATACACACATACCAGAAAAAGAAAGAAA
  GGATGGAGACGGAACATTTGCCTAATTTTGTAATAAAACACTGTCTTTTCAGGATTGCTT
  CATGGATTGGAGAACTTTCTAACCAAAAATTAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_002697
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_002697.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002697.2, NP_002688.2
• RefSeq Size: 2689 bp
• RefSeq ORF: 2232 bp
• Locus ID: 5451
• Cytogenetics: 1q24.2
• Domains: POU, homeobox
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.