PHKG2 (NM_000294) Human Untagged Clone

CAT#: SC321556

PHKG2 (untagged)-Human phosphorylase kinase, gamma 2 (testis) (PHKG2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHKG2
• Synonyms: GSD9C
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000294.1 CCGGCGACAGCGCAGCTCGCGTCGACCCTGGCTCCTCTGCCTGCCCCCTCAGGCCCCCGC
  CTCCTTCAGGATGACGCTGGACGTGGGGCCGGAGGATGAGCTGCCCGACTGGGCCGCCGC
  CAAAGAGTTTTACCAGAAGTACGACCCTAAGGACGTCATCGGCAGAGGAGTGAGCTCTGT
  GGTCCGCCGTTGTGTTCATCGAGCTACTGGCCACGAGTTTGCGGTGAAGATTATGGAAGT
  GACAGCTGAGCGGCTGAGTCCTGAGCAGCTGGAGGAGGTGCGGGAAGCCACACGGCGAGA
  GACACACATCCTTCGCCAGGTCGCCGGCCACCCCCACATCATCACCCTCATCGATTCCTA
  CGAGTCTTCTAGCTTCATGTTCCTGGTGTTTGACCTGATGCGGAAGGGAGAGCTGTTTGA
  CTATCTCACAGAGAAGGTGGCCCTCTCTGAAAAGGAAACCAGGTCCATCATGCGGTCTCT
  GCTGGAAGCAGTGAGCTTTCTCCATGCCAACAACATTGTGCATCGAGATCTGAAGCCCGA
  GAATATTCTCCTAGATGACAATATGCAGATCCGACTTTCAGATTTCGGGTTCTCCTGCCA
  CTTGGAACCTGGCGAGAAGCTTCGAGAGTTGTGTGGGACCCCAGGGTATCTAGCGCCAGA
  GATCCTTAAATGCTCCATGGATGAAACCCACCCAGGCTATGGCAAGGAGGTCGACCTCTG
  GGCCTGTGGGGTGATCTTGTTCACACTCCTGGCTGGCTCGCCACCCTTCTGGCACCGGCG
  GCAGATCCTGATGTTACGCATGATCATGGAGGGCCAGTACCAGTTCAGTTCCCCCGAGTG
  GGATGACCGTTCCAGCACTGTCAAAGACCTGATCTCCAGGCTGCTGCAGGTGGATCCTGA
  GGCACGCCTGACAGCTGAGCAGGCCCTACAGCACCCCTTCTTTGAGCGTTGTGAAGGCAG
  CCAACCCTGGAACCTCACCCCCCGCCAGCGGTTCCGGGTGGCAGTGTGGACAGTGCTGGC
  TGCTGGACGAGTGGCCCTAAGCACCCATCGTGTACGGCCACTGACCAAGAATGCACTGTT
  GAGGGACCCTTATGCGCTGCGGTCAGTGCGGCACCTCATCGACAACTGTGCCTTCCGGCT
  CTACGGGCACTGGGTAAAGAAAGGGGAGCAGCAGAACCGGGCGGCTCTCTTTCAGCACCG
  GCCCCCTGGGCCTTTTCCCATCATGGGCCCTGAAGAGGAGGGAGACTCTGCTGCTATAAC
  TGAGGATGAGGCCGTGCTTGTGCTGGGCTAGGACCTCAACCCCAGGGATTCCCAGGAAGC
  AGAACTCTCCAGAAGAAGGGTTTTGATCATTCCAGCTCCTCTGGGCTCTGGCCTCAGGCC
  CACTAATGATCCTGCTACCCTCTTGAAGACCAGCCCGGTACCTCTCTCCCCACTGGCCAG
  GACTCTGAGATCAGAGCTGGGGTGGAAGGGAGCCATTCTGAACGCCACGCCTGGCCCGGT
  CAGTGCTGCATGCACTGCATATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000294
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000294.1, NP_000285.1
• RefSeq Size: 1571 bp
• RefSeq ORF: 1221 bp
• Locus ID: 5261
• Cytogenetics: 16p11.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• Gene Summary: 'Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.