PHKG2 (NM_000294) Human Untagged Clone

CAT#: SC323429

PHKG2 (untagged)-Kinase deficient mutant (K53M) of Human phosphorylase kinase, gamma 2 (testis) (PHKG2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHKG2
• Synonyms: GSD9C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323429 sequence for NM_000294 edited (data generated by NextGen Sequencing)
  ATGACGCTGGACGTGGGGCCGGAGGATGAGCTGCCCGACTGGGCCGCCGCCAAAGAGTTT
  TACCAGAAGTACGACCCTAAGGACGTCATCGGCAGAGGAGTGAGCTCTGTGGTCCGCCGT
  TGTGTTCATCGAGCTACTGGCCACGAGTTTGCGGTGAAGATTATGGAAGTGACAGCTGAG
  CGGCTGAGTCCTGAGCAGCTGGAGGAGGTGCGGGAAGCCACACGGCGAGAGACACACATC
  CTTCGCCAGGTCGCCGGCCACCCCCACATCATCACCCTCATCGATTCCTACGAGTCTTCT
  AGCTTCATGTTCCTGGTGTTTGACCTGATGCGGAAGGGAGAGCTGTTTGACTATCTCACA
  GAGAAGGTGGCCCTCTCTGAAAAGGAAACCAGGTCCATCATGCGGTCTCTGCTGGAAGCA
  GTGAGCTTTCTCCATGCCAACAACATTGTGCATCGAGATCTGAAGCCCGAGAATATTCTC
  CTAGATGACAATATGCAGATCCGACTTTCAGATTTCGGGTTCTCCTGCCACTTGGAACCT
  GGCGAGAAGCTTCGAGAGTTGTGTGGGACCCCAGGGTATCTAGCGCCAGAGATCCTTAAA
  TGCTCCATGGATGAAACCCACCCAGGCTATGGCAAGGAGGTCGACCTCTGGGCCTGTGGG
  GTGATCTTGTTCACACTCCTGGCTGGCTCGCCACCCTTCTGGCACCGGCGGCAGATCCTG
  ATGTTACGCATGATCATGGAGGGCCAGTACCAGTTCAGTTCCCCCGAGTGGGATGACCGT
  TCCAGCACTGTCAAAGACCTGATCTCCAGGCTGCTGCAGGTGGATCCTGAGGCACGCCTG
  ACAGCTGAGCAGGCCCTACAGCACCCCTTCTTTGAGCGTTGTGAAGGCAGCCAACCCTGG
  AACCTCACCCCCCGCCAGCGGTTCCGGGTGGCAGTGTGGACAGTGCTGGCTGCTGGACGA
  GTGGCCCTAAGCACCCATCGTGTACGGCCACTGACCAAGAATGCACTGTTGAGGGACCCT
  TATGCGCTGCGGTCAGTGCGGCACCTCATCGACAACTGTGCCTTCCGGCTCTACGGGCAC
  TGGGTAAAGAAAGGGGAGCAGCAGAACCGGGCGGCTCTCTTTCAGCACCGGCCCCCTGGG
  CCTTTTCCCATCATGGGCCCTGAAGAGGAGGGAGACTCTGCTGCTATAACTGAGGATGAG
  GCCGTGCTTGTGCTGGGCTAG
  
  Clone variation with respect to NM_000294.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_000294 unedited
  ACCGCCCGTTTGAGCAAATGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCAGCTCG
  CGTCGACCCTGGCTCCTCTGCCTGCCCCCTCAGGCCCCCGCCTCCTTCAGGATGACGCTGGACGTGGGGC
  CGGAGGATGAGCTGCCCGACTGGGCCGCCGCCAAAGAGTTTTACCAGAAGTACGACCCTAAGGACGTCAT
  CGGCAGAGGAGTGAGCTCTGTGGTCCCGCCGTTGTGTTCATCGAGCTACTGGGCCACGAGTTTTGCGGGT
  GATGATTATGGGAAGTGACAGCTGAGCGGGCTGAGTTCCTGGAGCAGCTGGGAGGAAGGGGCCGGGAGCC
  CACACGGCAAGAGAACACACATCCCTTCGCAAGGTCGGCCGGCCACCCCCCACATAATCCCCCTCATCAA
  TTCTAAGAAGTCTTTCTGGCTCCAGGTTCCGGGGGTTGGACTTGTGGGCGGAAGGAGAACCTGTTGGACT
  ACCTAACGAAAAAAGTGGCCCCCTCTCGAAAAAGGAAAAAAGTCCCCCATGCGCGTCCCTCTTTTGAGAG
  CGTTGAGCTTCTTCTATGCAAACCCACTTTTGTCCTGAAGATCTAGAGCCGGAAAATTCTCTCTGATGAC
  ACAATGCGATACGCATTTTACATTCGGGTTCTGCGCCTGGAACCCGCGAGAGACTCTAAGTGTGTGTGGC
  CCAAGGATCATCGCCAGATACTCAAAGTCCTGTGAGACACCGCGCTGCGAGAGGTCACACCTGGCCGTGG
  GGATCTGTACACTCGTGTCGCGCACCTTTTGCCGCGGATCGAGTGCGACTCAGGACTCAGCTAGTCCAGT
  GTAGAGCATCACCTGAACGATTCACGGTGGAG
  
  Kinase domain sequence

  >SC323429 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSMTKMGCAATGGGCGKAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCAGCTCGCGTCGACCCT
  GGCTCCTCTGCCTGCCCCCTCAGGCCCCCGCCTCCTTCAGGATGACGCTGGACGTGGGGCCGGAGGATGA
  GCTGCCCGACTGGGCCGCCGCCAAAGAGTTTTACCAGAAGTACGA
  
• Restriction Sites: Please inquire
• ACCN: NM_000294
• Insert Size: 1640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000294.1, NP_000285.1
• RefSeq Size: 1571 bp
• RefSeq ORF: 1221 bp
• Locus ID: 5261
• Cytogenetics: 16p11.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• Gene Summary: 'Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.