NFIC (NM_005597) Human Untagged Clone

CAT#: SC321627

NFIC (untagged)-Human nuclear factor I/C (CCAAT-binding transcription factor) (NFIC), transcript variant 1


  "NM_005597" in other vectors (6)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NFIC
Synonyms CTF; CTF5; NF-I; NFI
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_005597.2 AATGACTCAGTAAGTTCAGCGCGCCCGCTCCGGCCGGCCCTGCGCCTCCCGCCGCGCCCG
GGATGTATTCGTCCCCGCTCTGCCTCACCCAGGATGAGTTCCACCCGTTCATCGAGGCCC
TGCTGCCTCACGTCCGCGCCTTCGCCTACACCTGGTTCAACCTGCAGGCGCGGAAGCGCA
AGTACTTCAAGAAGCACGAGAAGCGGATGTCGAAGGACGAGGAGCGTGCGGTCAAGGACG
AGCTGCTGGGCGAGAAGCCCGAGGTCAAGCAGAAGTGGGCGTCGCGGCTGCTGGCCAAGC
TGCGCAAGGACATCCGGCCCGAGTGCCGCGAGGACTTCGTGCTGAGCATCACCGGCAAGA
AGGCGCCGGGCTGCGTGCTCTCCAACCCCGACCAGAAGGGCAAGATGCGGCGCATCGACT
GTCTCCGGCAGGCGGACAAGGTGTGGCGGCTGGACCTGGTCATGGTCATCCTGTTCAAGG
GCATCCCGCTGGAGAGCACCGACGGCGAGCGCCTGGTCAAGGCTGCGCAGTGCGGTCACC
CGGTCCTGTGCGTGCAGCCGCACCACATTGGCGTGGCCGTCAAGGAGCTGGACCTCTACC
TGGCCTACTTCGTGCGTGAGCGAGATGCAGAGCAAAGCGGCAGTCCCCGGACAGGGATGG
GCTCTGACCAGGAGGACAGCAAGCCCATCACGCTGGACACGACCGACTTCCAGGAGAGCT
TTGTCACCTCCGGCGTGTTCAGCGTCACTGAGCTCATCCAAGTGTCCCGGACACCCGTGG
TGACTGGAACAGGACCCAACTTCTCCCTGGGGGAGCTGCAGGGGCACCTGGCATACGACC
TGAACCCAGCCAGCACTGGCCTCAGAAGAACGCTGCCCAGCACCTCCTCCAGTGGGAGCA
AGCGGCACAAATCGGGCTCGATGGAGGAAGACGTGGACACGAGCCCTGGCGGCGATTACT
ACACTTCGCCCAGCTCGCCCACGAGTAGCAGCCGCAACTGGACGGAGGACATGGAAGGAG
GCATCTCGTCCCCGGTGAAGAAGACAGAGATGGACAAGTCACCATTCAACAGCCCGTCCC
CCCAGGACTCTCCCCGCCTCTCCAGCTTCACCCAGCACCACCGGCCCGTCATCGCCGTGC
ACAGCGGGATCGCCCGGAGCCCACACCCGTCCTCCGCTCTGCATTTCCCTACGACGTCCA
TCCTACCCCAGACGGCCTCCACCTACTTCCCCCACACGGCCATCCGCTACCCACCTCATC
TCAACCCCCAGGACCCGCTCAAAGATCTTGTCTCGCTGGCCTGCGACCCAGCCAGCCAGC
AACCTGGACCGTCCTGGTATCTGGGATAGCAAAGGTCTTCTTCCCTCGCCCCTTCTCCAT
CGTCCCAGGAATCCCAGGGGGCAGCACAGCCGGCCCCCGGCCCACGTTTTCGGTGGAAAA
TTAGAGTGAACAAGAACACCCCTGCCGACTCCCAGCCCGGCCAAAAAGACAAAACACATA
GACGCACACACTCAGGAGGAAAAGAAAAAACAAAGGCAGAAGAAGAAAAAAAAAAAAAAA
AAA
Restriction Sites Please inquire     
ACCN NM_005597
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005597.2, NP_005588.2
RefSeq Size 2185 bp
RefSeq ORF 1287 bp
Locus ID 4782
Cytogenetics 19p13.3
Protein Families Transcription Factors
Gene Summary 'The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]'
Transcript Variant: This variant (5) lacks two consecutive in-frame coding exons compared to variant 1. This results in a shorter isoform (5) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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