GLRB (NM_000824) Human Untagged Clone

CAT#: SC321743

GLRB (untagged)-Human glycine receptor, beta (GLRB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLRB
• Synonyms: HKPX2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000824.2 CTGGGACGCAGCTGCCCCCGCTCGGCGCCCGCTGCACCCTTAGCAGCCACTGCCACCTGG
  GCCCGGAGCCTCCACGATCTCGCCCGGCGATTGTGGGCAGGGGCGCCTCCGGATCGATCT
  TCTGAAATTCAAGTTTTCAAGATGAAGTTTTTATTGACAACTGCCTTTTTAATTTTAATT
  TCCTTGTGGGTGGAAGAAGCCTATTCTAAGGAAAAGTCTTCAAAGAAAGGGAAGGGGAAA
  AAGAAGCAGTATCTATGCCCATCTCAGCAGTCAGCAGAGGACCTTGCCCGAGTACCTGCC
  AACTCCACTAGCAATATCTTGAACAGGTTATTGGTCAGTTATGATCCCAGGATAAGACCA
  AACTTCAAAGGCATTCCTGTTGATGTAGTAGTCAACATTTTTATTAACAGTTTTGGATCC
  ATTCAAGAAACAACAATGGACTATAGAGTTAACATCTTCCTGAGACAAAAATGGAATGAC
  CCCAGGCTGAAGCTCCCCAGTGATTTTAGGGGTTCAGATGCACTGACAGTGGATCCAACA
  ATGTACAAGTGTTTATGGAAACCTGATTTATTTTTTGCAAATGAAAAAAGTGCCAATTTT
  CATGATGTGACCCAGGAAAACATCCTCCTCTTTATTTTTCGTGATGGAGATGTCCTTGTC
  AGCATGAGGTTATCTATTACTCTTTCATGCCCTTTGGACTTGACATTGTTTCCCATGGAT
  ACACAACGTTGCAAGATGCAACTGGAGAGCTTTGGTTACACAACTGATGATTTACGATTT
  ATCTGGCAGTCAGGAGATCCTGTGCAATTAGAAAAAATTGCCTTGCCTCAATTTGATATC
  AAAAAGGAAGATATTGAATATGGTAACTGTACAAAATACTATAAAGGCACGGGCTACTAC
  ACATGCGTGGAAGTCATCTTCACCCTGAGGAGGCAGGTCGGCTTTTACATGATGGGGGTC
  TACGCCCCAACCCTGCTCATTGTTGTTCTCTCCTGGCTTTCCTTCTGGATCAACCCGGAC
  GCGAGTGCTGCCAGAGTGCCCCTGGGTATCTTCTCAGTCCTCAGCTTGGCCTCTGAGTGC
  ACAACCCTTGCCGCTGAGCTTCCCAAAGTTTCCTATGTGAAGGCTCTTGATGTTTGGCTT
  ATTGCTTGCCTTCTCTTTGGGTTTGCTTCCCTGGTGGAGTATGCAGTTGTCCAGGTGATG
  CTGAACAACCCCAAAAGGGTTGAAGCTGAAAAAGCCAGAATTGCTAAGGCTGAGCAAGCA
  GATGGAAAAGGTGGAAATGTGGCTAAAAAGAATACTGTGAATGGAACAGGGACTCCTGTT
  CATATTAGCACTTTGCAGGTTGGTGAGACCAGATGCAAAAAAGTTTGTACTTCTAAGTCT
  GATCTGAGATCTAATGACTTCAGCATTGTTGGAAGCTTACCAAGAGATTTTGAACTATCC
  AATTATGACTGCTATGGAAAACCCATTGAAGTTAACAACGGACTTGGGAAATCTCAGGCT
  AAGAACAACAAGAAGCCTCCCCCTGCGAAACCTGTTATTCCAACAGCAGCAAAGCGAATT
  GATCTTTATGCAAGAGCATTGTTTCCTTTCTGCTTCTTGTTCTTCAATGTTATATATTGG
  TCTATATATTTATGATAAATCTTTTCCATTTGTACAAAATAAAATTCCATTTCATTGTGA
  CCTACTCCTTTCATAAATGCCAATCTGTGAGAACTTTTGAATTTTCATAGCAACATTGCA
  TTTTGGATGCCATTTGATTGTAATAAAACTGTGGCACCTTAATTTTGAATGGCAGCATGA
  TCATGTAATATCTGTGCTCTAATAACGATGTATATATGTATAGTGAACATATTGCTTAGT
  AACAAATGAAGGACAAGCATACTACATAATATAATCCATACAATTCTCTTCAGTTAGTGT
  AAACTGCAAATACTACAGATAATTCTGATAATAAAATGATATGCACGCTGAATCCTGCTA
  TGGTCACCATTCTAATGTATGTAGTATTTCAAATTTCCTTCCTTGTAACTTTCAAAGAAA
  GCCATCTTATTCTTGTAATTTTAGATGGTATTATCACAGATTTAAAAAGGTTGTATTACA
  TATTGTTTAAACTTTGTAAGTAGAAATATATCTGTTATAATTATACAGGCTCTGTGGAGA
  AATAAAGTTCAAAATATATTAATTTGTAAAATCAGCTCGTTTTAAAGTGTGCTTGTGTTG
  TCAAAAATATCAGATAGTAATACACAGTGAGCATTTTTAAACAAAGGGAAACCTATATTT
  ATGTAACTGTATACTGAATTCTGACAAAATAAAAAAAGATACCTTATTGACGAAATATTT
  AGGATAAACAAAATTCTATTTAATCCACCTTAAAACCTAAATGTATTTTCATGGATTTCA
  TTTGTTGGTACATATTACACAAAACATTGTGCCTTAAAATGAGTCATACATCTTTTAAAT
  TGGAATGCAGTAATAGATATGTGATTTTACATCATTTTTAAGAAACCAAGGGGAAGTAAT
  AAGTTGAAAAAGAAATCCATAACTATTAAAAGATTTTAACTTTTTTATTTTATTAAAATG
  CTTGCATATTTTAAGTAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000824
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000824.2, NP_000815.1
• RefSeq Size: 2649 bp
• RefSeq ORF: 1494 bp
• Locus ID: 2743
• Cytogenetics: 4q32.1
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (1, also known as variant A) represents the longest transcript and encodes the longer isoform (A). Both variants 1 and 2 encode the same isoform.