KPNA2 (NM_002266) Human Untagged Clone

CAT#: SC321886

KPNA2 (untagged)-Human karyopherin alpha 2 (RAG cohort 1, importin alpha 1) (KPNA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KPNA2
• Synonyms: IPOA1; QIP2; RCH1; SRP1-alpha; SRP1alpha
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002266.2 GAGCTGAGTCGAGGTGGACCCTTTGAACGCAGTCGCCCTACAGCCGCTGATTCCCCCCGC
  ATCGCCTCCCGTGGAAGCCCAGGCCCGCTTCGCAGCTTTCTCCCTTTGTCTCATAACCAT
  GTCCACCAACGAGAATGCTAATACACCAGCTGCCCGTCTTCACAGATTCAAGAACAAGGG
  AAAAGACAGTACAGAAATGAGGCGTCGCAGAATAGAGGTCAATGTGGAGCTGAGGAAAGC
  TAAGAAGGATGACCAGATGCTGAAGAGGAGAAATGTAAGCTCATTTCCTGATGATGCTAC
  TTCTCCGCTGCAGGAAAACCGCAACAACCAGGGCACTGTAAATTGGTCTGTTGATGACAT
  TGTCAAAGGCATAAATAGCAGCAATGTGGAAAATCAGCTCCAAGCTACTCAAGCTGCCAG
  GAAACTACTTTCCAGAGAAAAACAGCCCCCCATAGACAACATAATCCGGGCTGGTTTGAT
  TCCGAAATTTGTGTCCTTCTTGGGCAGAACTGATTGTAGTCCCATTCAGTTTGAATCTGC
  TTGGGCACTCACTAACATTGCTTCTGGGACATCAGAACAAACCAAGGCTGTGGTAGATGG
  AGGTGCCATCCCAGCATTCATTTCTCTGTTGGCATCTCCCCATGCTCACATCAGTGAACA
  AGCTGTCTGGGCTCTAGGAAACATTGCAGGTGATGGCTCAGTGTTCCGAGACTTGGTTAT
  TAAGTACGGTGCAGTTGACCCACTGTTGGCTCTCCTTGCAGTTCCTGAGATGTCATCTTT
  AGCATGTGGCTACTTACGTAATCTTACCTGGACACTTTCTAATCTTTGCCGCAACAAGAA
  TCCTGCACCCCCGATAGATGCTGTTGAGCAGATTCTTCCTACCTTAGTTCGGCTCCTGCA
  TCATGATGATCCAGAAGTGTTAGCAGATACCTGCTGGGCTATTTCCTACCTTACTGATGG
  TCCAAATGAACGAATTGGCATGGTGGTGAAAACAGGAGTTGTGCCCCAACTTGTGAAGCT
  TCTAGGAGCTTCTGAATTGCCAATTGTGACTCCTGCCCTAAGAGCCATAGGGAATATTGT
  CACTGGTACAGATGAACAGACTCAGGTTGTGATTGATGCAGGAGCACTCGCCGTCTTTCC
  CAGCCTGCTCACCAACCCCAAAACTAACATTCAGAAGGAAGCTACGTGGACAATGTCAAA
  CATCACAGCCGGCCGCCAGGACCAGATACAGCAAGTTGTGAATCATGGATTAGTCCCATT
  CCTTGTCAGTGTTCTCTCTAAGGCAGATTTTAAGACACAAAAGGAAGCTGTGTGGGCCGT
  GACCAACTATACCAGTGGTGGAACAGTTGAACAGATTGTGTACCTTGTTCACTGTGGCAT
  AATAGAACCGTTGATGAACCTCTTAACTGCAAAAGATACCAAGATTATTCTGGTTATCCT
  GGATGCCATTTCAAATATCTTTCAGGCTGCTGAGAAACTAGGTGAAACTGAGAAACTTAG
  TATAATGATTGAAGAATGTGGAGGCTTAGACAAAATTGAAGCTCTACAAAACCATGAAAA
  TGAGTCTGTGTATAAGGCTTCGTTAAGCTTAATTGAGAAGTATTTCTCTGTAGAGGAAGA
  GGAAGATCAAAACGTTGTACCAGAAACTACCTCTGAAGGCTACACTTTCCAAGTTCAGGA
  TGGGGCTCCTGGGACCTTTAACTTTTAGATCATGTAGCTGAGACATAAATTTGTTGTGTA
  CTACGTTTGGTATTTTGTCTTATTGTTTCTCTACTAAGAACTCTTTCTTAAATGTGGTTT
  GTTACTGTAGCACTTTTTACACTGAAACTATACTTGAACAGTTCCAACTGTACATACATA
  CTGTATGAAGCTTGTCCTCTGACTAGGTTTCTAATTTCTATGTGGAATTTCCTATCTTGC
  AGCATCCTGTAAATAAACATTCAAGTCCACCCTTTTCTTGACTTCAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002266
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002266.2, NP_002257.1
• RefSeq Size: 2011 bp
• RefSeq ORF: 1590 bp
• Locus ID: 3838
• Cytogenetics: 17q24.2
• Domains: Armadillo_seg, IBB
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: 'The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.