Angiotensinogen (AGT) (NM_000029) Human Untagged Clone

CAT#: SC322276

AGT (untagged)-Human angiotensinogen (serpin peptidase inhibitor, clade A, member 8) (AGT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AGT
• Synonyms: ANHU; hFLT1; SERPINA8
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322276 GTTGTTCTGGGTACTACAGCAGAAGGGTATGCGGAAGCGAGCACCCCAGTCTGAGATGGC
  TCCTGCCGGTGTGAGCCTGAGGGCCACCATCCTCTGCCTCCTGGCCTGGGCTGGCCTGGC
  TGCAGGTGACCGGGTGTACATACACCCCTTCCACCTCGTCATCCACAATGAGAGTACCTG
  TGAGCAGCTGGCAAAGGCCAATGCCGGGAAGCCCAAAGACCCCACCTTCATACCTGCTCC
  AATTCAGGCCAAGACATCCCCTGTGGATGAAAAGGCCCTACAGGACCAGCTGGTGCTAGT
  CGCTGCAAAACTTGACACCGAAGACAAGTTGAGGGCCGCAATGGTCGGGATGCTGGCCAA
  CTTCTTGGGCTTCCGTATATATGGCATGCACAGTGAGCTATGGGGCGTGGTCCATGGGGC
  CACCGTCCTCTCCCCAACGGCTGTCTTTGGCACCCTGGCCTCTCTCTATCTGGGAGCCTT
  GGACCACACAGCTGACAGGCTACAGGCAATCCTGGGTGTTCCTTGGAAGGACAAGAACTG
  CACCTCCCGGCTGGATGCGCACAAGGTCCTGTCTGCCCTGCAGGCTGTACAGGGCCTGCT
  AGTGGCCCAGGGCAGGGCTGATAGCCAGGCCCAGCTGCTGCTGTCCACGGTGGTGGGCGT
  GTTCACAGCCCCAGGCCTGCACCTGAAGCAGCCGTTTGTGCAGGGCCTGGCTCTCTATAC
  CCCTGTGGTCCTCCCACGCTCTCTGGACTTCACAGAACTGGATGTTGCTGCTGAGAAGAT
  TGACAGGTTCATGCAGGCTGTGACAGGATGGAAGACTGGCTGCTCCCTGACGGGAGCCAG
  TGTGGACAGCACCCTGGCTTTCAACACCTACGTCCACTTCCAAGGGAAGATGAAGGGCTT
  CTCCCTGCTGGCCGAGCCCCAGGAGTTCTGGGTGGACAACAGCACCTCAGTGTCTGTTCC
  CATGCTCTCTGGCATGGGCACCTTCCAGCACTGGAGTGACATCCAGGACAACTTCTCGGT
  GACTCAAGTGTCCTTCACTGAGAGCGCCTGCCTGCTGCTGATCCAGCCTCACTATGCCTC
  TGACCTGGACAAGGTGGAGGGTCTCACTTTCCAGCAAAACTCCCTCAACTGGATGAAGAA
  ACTGTCTCCCCGGACCATCCACCTGACCATGCCCCAACTGGTGCTGCAAGGATCTTATGA
  CCTGCAGGACCTGCTCGCCCAGGCTGAGCTGCCCGCCATTCTGCACACCGAGCTGAACCT
  GCAAAAATTGAGCAATGACCGCATCAGGGTGGGGGAGGTGCTGAACAGCATTTTTTTTGA
  GCTTGAAGCGGATGAGAGAGAGCCCACAGAGTCTACCCAACAGCTTAACAAGCCTGAGGT
  CTTGGAGGTGACCCTGAACCGCCCATTCCTGTTTGCTGTGTATGATCAAAGCGCCACTGC
  CCTGCACTTCCTGGGCCGCGTGGCCAACCCGCTGAGCACAGCATGAGGCCAGGGCCCCAG
  AACACAGTGCCTGGCAAGGCCTCTGCCCCTGGCCTTTGAGGCAAAGGCCAGCAGCAGATA
  ACAACCCCGGACAAATCAGCGATGTGTCACCCCCAGTCTCCCACCTTTTCTTCTAATGAG
  TCGACTTTGAGCTGGAAAGCAGCCGTTTCTCCTTGGTCTAAGTGTGCTGCATGGAGTGAG
  CAGTAGAAGCCTGCAGCGGCACAAATGCACCTCCCAGTTTGCTGGGTTTATTTTAGAGAA
  TGGGGGTGGGGAGGCAAGAACCAGTGTTTAGCGCGGGACTACTGTTCCAAAAAGAATTCC
  AACCGACCAGCTTGTTTGTGAAACAAAAAAGTGTTCCCTTTTCAAGTTGAGAACAAAAAT
  TGGGTTTTAAAATTAAAGTATACATTTTTGCATTGCAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000029
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000029.2, NP_000020.1
• RefSeq Size: 2192 bp
• RefSeq ORF: 1458 bp
• Locus ID: 183
• Cytogenetics: 1q42.2
• Domains: SERPIN
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Renin-angiotensin system
• Gene Summary: 'The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure, body fluid and electrolyte homeostasis, and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Nov 2019]'