MRF (MYRF) (NM_001127392) Human Untagged Clone

CAT#: SC323261

MYRF (untagged)-Human chromosome 11 open reading frame 9 (C11orf9), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYRF
• Synonyms: 11orf9; C11orf9; CUGS; MMERV; MRF; Ndt80; pqn-47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001127392, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGTGGTGGACGAGACGGAGGCGCTGCAGCGCTTCTTCGAAGGCCACGACATCAACGGTGCCCTGG
  AGCCCTCCAACATAGACACCAGCATCCTGGAGGAGTACATCAGCAAGGAGGATGCCTCCGACCTCTGCTT
  CCCTGACATCTCTGCTCCAGCCAGCTCGGCCTCCTACTCCCACGGGCAGCCTGCGATGCCTGGCTCCAGC
  GGGGTCCACCACCTGAGCCCCCCTGGGGGTGGACCCTCCCCGGGGCGCCATGGTCCCCTCCCACCCCCGG
  GCTACGGCACCCCGCTGAACTGCAACAACAACAACGGCATGGGCGCTGCCCCCAAGCCCTTCCCGGGGGG
  CACCGGGCCCCCCATCAAGGCTGAGCCCAAGGCTCCCTATGCCCCAGGCACACTGCCGGACTCTCCCCCA
  GACTCGGGCTCCGAGGCCTACTCCCCCCAGCAGGTGAATGAGCCCCACCTCCTGCGCACGATAACCCCTG
  AGACACTGTGCCACGTGGGAGTGCCCTCCCGCCTGGAGCATCCGCCCCCACCTCCAGCCCACTTGCCAGG
  CCCCCCGCCACCCCCACCACCCCCACCTCACTACCCTGTCCTGCAGCGGGATCTGTACATGAAGGCCGAG
  CCCCCGATCCCCCACTACGCTGCCATGGGGCAGGGGCTGGTGCCCACTGATCTTCACCACACCCAGCAGT
  CCCAGATGCTGCACCAGCTCCTGCAGCAGCACGGAGCTGAGCTCCCTACACACCCCTCCAAGAAGAGGAA
  GCACTCTGAATCCCCCCCCAGCACCCTCAATGCCCAGATGCTGAATGGAATGATCAAACAGGAGCCTGGG
  ACCGTGACAGCCCTGCCTCTGCACCCCACTCGAGCCCCATCGCCACCCTGGCCTCCCCAGGGTCCGCTCT
  CCCCGGGCCCTGGTTCCTTGCCTCTCAGCATTGCCCGTGTCCAGACACCGCCTTGGCACCCGCCAGGTGC
  CCCCTCCCCAGGCCTCCTGCAGGACAGTGACAGCCTCAGTGGCTCCTACCTGGACCCCAACTACCAGTCC
  ATCAAGTGGCAGCCTCATCAGCAGAACAAGTGGGCGACCCTGTACGATGCTAACTACAAGGAGCTGCCCA
  TGCTCACCTACCGCGTGGATGCGGACAAGGGCTTCAACTTTTCGGTGGGCGACGACGCCTTTGTGTGCCA
  GAAGAAGAACCACTTCCAGGTGACAGTGTACATCGGCATGCTGGGCGAGCCCAAGTACGTCAAGACGCCC
  GAGGGCCTCAAGCCCCTCGACTGCTTCTATCTGAAGCTGCACGGAGTGAAGCTGGAGGCCCTGAACCAGT
  CCATTAACATCGAGCAGTCCCAGTCAGACCGGAGCAAGCGGCCCTTCAACCCGGTCACGGTCAATCTGCC
  CCCTGAGCAGGTCACGAAGGTGACTGTGGGGCGGCTGCACTTCAGCGAGACCACCGCTAACAACATGCGT
  AAGAAGGGCAAGCCCAACCCGGACCAGAGGTACTTCATGCTGGTGGTGGCCCTCCAGGCTCATGCACAGA
  ACCAGAACTACACGCTGGCCGCCCAGATCTCAGAGCGCATCATTGTGCGGGCCTCCAACCCAGGCCAGTT
  CGAGAGCGACAGCGATGTGTTGTGGCAGCGGGCACAGGTGCCCGACACCGTCTTCCACCACGGCCGCGTG
  GGCATCAACACAGACCGGCCGGATGAGGCGCTGGTTGTGCACGGGAATGTCAAGGTCATGGGCTCGCTTA
  TGCACCCCTCCGACCTGCGCGCCAAGGAACACGTGCAGGAGGTGGACACCACCGAGCAATTGAAGAGGAT
  CTCGCGCATGCGGCTGGTGCACTACAGATACAAGCCCGAGTTCGCCGCCAGCGCGGGCATCGAGGCCACC
  GCGCCAGAGACAGGTGTCATCGCTCAGGAGGTGAAGGAGATCTTGCCTGAGGCTGTGAAAGACACCGGAG
  ACATGGTCTTTGCCAATGGGAAAACCATAGAGAACTTCCTGGTGGTGAACAAGGAGCGCATCTTCATGGA
  GAACGTAGGGGCCGTGAAGGAGCTGTGCAAGCTGACAGACAACCTGGAGACGCGCATTGATGAGCTGGAG
  CGCTGGAGCCACAAGCTGGCCAAGCTGCGGCGGCTCGACAGCCTCAAGTCCACCGGCAGCTCGGGCGCCT
  TCAGCCATGCAGGGAGCCAGTTCAGTCGGGCGGGCAGCGTCCCCCACAAGAAGAGGCCCCCCAAGGTGGC
  CAGCAAGTCATCGTCCGTGGTTCCGGACCAGGCCTGCATCAGCCAGCGCTTCCTGCAGGGAACCATCATT
  GCCCTGGTGGTGGTCATGGCCTTCAGCGTGGTGTCCATGTCCACACTGTACGTGCTGAGCCTGCGCACAG
  AGGAGGACCTGGTAGACACTGATGGCTCTTTTGCCGTGTCCACTTCCTGTCTCCTGGCCCTGCTCCGGCC
  CCAGCCCCCTGGGGGGAGTGAGGCCTTGTGCCCATGGTCCAGCCAGAGCTTTGGGACCACGCAGCTCCGA
  CAGTCCCCCTTGACCACGGGGCTACCAGGCATACAGCCCTCTTTGCTGCTGGTGACCACCAGCCTCACCA
  GCTCGGCCCCAGGTTCTGCTGTCCGCACCTTGGACATGTGTTCCAGCCACCCCTGCCCTGTCATCTGCTG
  TTCCTCACCCACTACCAACCCTACCACTGGTCCTAGTCTTGGCCCCAGCTTTAACCCTGGCCATGTTCTC
  AGCCCAAGTCCCAGCCCCAGCACCAACCGCTCAGGCCCCAGCCAGATGGCCCTTCTGCCAGTCACCAACA
  TCAGAGCCAAGTCCTGGGGTCTTTCAGTCAATGGCATTGGCCACTCCAAGCATCACAAGAGTCTGGAGCC
  TCTGGCCAGCCCTGCAGTCCCCTTCCCTGGGGGGCAGGGCAAAGCCAAGAACAGTCCCAGCCTTGGTTTC
  CATGGCCGGGCCCGCCGAGGGGCCCTCCAGTCCAGCGTGGGCCCTGCTGAGCCCACCTGGGCCCAGGGCC
  AGTCAGCCTCTCTCCTTGCAGAGCCAGTGCCCTCCCTGACCTCCATCCAGGTGCTGGAGAATTCGATGTC
  CATCACCTCCCAGTACTGTGCTCCAGGGGATGCCTGCAGGCCTGGGAACTTCACCTACCACATCCCTGTC
  AGTAGTGGCACCCCACTGCACCTCAGCCTGACTCTGCAGATGAACTCCTCCTCCCCCGTGTCTGTGGTGC
  TGTGCAGCCTGAGGTCAAAGGAGGAACCATGTGAGGAGGGGAGCCTTCCACAGAGTCTCCACACCCACCA
  GGACACCCAGGGCACCTCTCACCGGTGGCCAATAACCATCCTGTCCTTCCGTGAATTCACCTACCACTTC
  CGGGTGGCACTGCTGGGTCAGGCCAACTGCAGTTCAGAGGCTCTCGCCCAGCCAGCCACAGACTACCACT
  TCCACTTCTACCGCCTGTGTGACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001127392
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127392.2, NP_001120864.1
• RefSeq Size: 5949 bp
• RefSeq ORF: 3456 bp
• Locus ID: 745
• Cytogenetics: 11q12.2
• Gene Summary: 'This gene encodes a transcription factor that is required for central nervous system myelination and may regulate oligodendrocyte differentiation. It is thought to act by increasing the expression of genes that effect myelin production but may also directly promote myelin gene expression. Loss of a similar gene in mouse models results in severe demyelination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]'
  Transcript Variant: This variant (2) lacks a 5' exon and uses an alternate 5' terminal exon, compared to variant 1. It encodes isoform 2 which is longer and has a distinct N-terminus compared to isoform 1.