PDIK1L (NM_152835) Human Untagged Clone

CAT#: SC323346

PDIK1L (untagged)-Kinase deficient mutant (K37M) of Human PDLIM1 interacting kinase 1 like (PDIK1L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDIK1L
• Synonyms: CLIK1L; STK35L2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323346 sequence for NM_152835 edited (data generated by NextGen Sequencing)
  ATGGTGAGTAGCCAGCCAAAGTACGATCTAATACGGGAGGTAGGCCGAGGTAGTTACGGT
  GTTGTGTATGAAGCAGTCATCAGAAAGACCTCTGCACGGGTGGCAGTGAWGAAAATTCGA
  TGTCACGCACCTGAAAATGTTGAACTAGCCCTTCGTGAGTTCTGGGCACTAAGCAGTATC
  AAGAGCCAACATCCAAATGTGATTCACTTGGAGGAATGCATCCTACAAAAGGATGGGATG
  GTGCAAAAGATGTCCCACGGCTCTAATTCTTCCCTTTATTTACAGCTTGTAGAAACTTCA
  TTAAAAGGAGAAATTGCCTTTGATCCCAGAAGCGCCTATTATTTGTGGTTTGTGATGGAT
  TTTTGTGACGGAGGAGATATGAATGAGTATCTGTTGTCCAGGAAACCCAATCGTAAAACT
  AACACCAGCTTCATGCTTCAGCTGAGCAGTGCCCTGGCTTTCTTGCATAAAAACCAGATC
  ATCCACCGAGATCTTAAGCCTGATAACATCCTGATTTCTCAAACCAGGTTGGATACCAGT
  GACTTGGAACCTACCCTCAAAGTGGCTGATTTTGGTCTAAGTAAAGTTTGTTCAGCCTCT
  GGGCAGAACCCAGAAGAACCTGTCAGTGTAAACAAGTGTTTCCTTTCCACAGCATGTGGA
  ACAGATTTTTACATGGCTCCTGAAGTTTGGGAAGGACATTACACAGCAAAAGCTGACATC
  TTTGCTCTGGGGATTATCATCTGGGCAATGCTGGAAAGGATCACATTCATAGACACAGAG
  ACAAAGAAGGAACTCTTGGGGAGTTATGTAAAACAAGGAACTGAGATTGTGCCTGTTGGG
  GAGGCACTTCTGGAAAATCCCAAAATGGAACTTCTCATTCCTGTGAAGAAAAAATCTATG
  AATGGGCGAATGAAACAACTGATTAAGGAAATGCTGGCTGCAAACCCTCAGGATCGTCCA
  GATGCTTTTGAACTAGAACTCAGATTAGTACAAATTGCATTTAAAGATAGCAGCTGGGAA
  ACGTGA
  
  Clone variation with respect to NM_152835.4
  110 a=>w
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_152835 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCAGCGGAGGCGC
  GAGCTGCCCGATAATGGCGGCCTGCAGAGCCCATGAGAGGGAGAAGCGGCAGCGTCTACCCTGAGGAAGG
  TGCTCTTCCCCATGGGGACTGTACCAGGCTGGAGAGGACATGGCACTGCTTCAGAGACACGAACCACCTT
  GAGTGGATGTGATTGGCGATCCCCGTAGAGCAGGAAAGCTCGTCTGCTGTGCAGCCGGCGCAGAGGATTC
  TGTGGCAGGAAATTAGACACTAGAAATTCAGTAGCGATTTGAAGGAAACCTCGACCTTGAGATGGTGAGT
  AGCCAGCCAAAGTACGATCTATACGGAGGTAGGCCGAGGTAGTTACGTTGTTGGTGTATGAGCAGTCATC
  AGAAGACCTCTGCCACGGTTGCCAGGTGATGAATTCGATTGTCCACCGCCACTTGAAATTGTTGACCTAG
  TCATCAGAAGGACCTCTCGGCACGGTGGCCATGTGAATGAAATCGATGTTCACGCCACCTTGAAATGTGT
  GACTTAGCCTTCCTAATCTCTGGACCTAGCGATATTCACGAGAGCCCACACCTCCAGTGTATTCATTGGA
  GGAATGTCCATCCTCTCACAGGATGGATGTGTCCAGAGTTCCACGCCTTATTCTTCGTTATTCAGCTGTG
  AAACTTCTTTAAAGGACTGCCTGATCCACGGCTATTTGTTTGTAGATTTTGCGGGGATATGAGATTCTTG
  TCCGAACATCTGAACATACGCTAGCTACTTGACATGCTGCGTCTGTCAACAGATATCCGGATTTATCCGG
  TAATCGATTTTACACTG
  
  Kinase domain sequence

  >SC323346 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ACGMTKMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCAGCGGAGGCGCGAGC
  TGCCCGATAATGGCGGCCTGCAGAGCCCATGAGAGGGAGAAGCGGCAGCGTCTACCCTGAGGAAGGTGCT
  CTTCCCCATGGGGACTGTACCAGGCTGGAGAGGACATGGCACTGC
  
• Restriction Sites: Please inquire
• ACCN: NM_152835
• ORF Size: 1026 bp
• Insert Size: 4700
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_152835.1, NP_690048.1
• RefSeq Size: 4299
• RefSeq ORF: 1026
• Locus ID: 149420
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 2 and 3 encode the same protein.