SGK2 (NM_170693) Human Untagged Clone

CAT#: SC323365

SGK2 (untagged)-Kinase deficient mutant (K64M) of Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SGK2
• Synonyms: dJ138B7.2; H-SGK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323365 sequence for NM_170693 edited (data generated by NextGen Sequencing)
  ATGAACTCTAGCCCAGCTGGGACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATC
  AACCTGGGGCCTTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTC
  ATCGGCAAAGGGAACTACGGGAAGGTCCTACTGGCCAAGCGCAAGTCTGATGGGGCGTTC
  TATGCAGTGATGGTACTACAGAAAAAGTCCATCTTAAAGAAGAAAGAGCAGAGCCACATC
  ATGGCAGAGCGCAGTGTGCTTCTGAAGAACGTGCGGCACCCCTTCCTCGTGGGCCTGCGC
  TACTCCTTCCAGACACCTGAGAAGCTCTACTTCGTGCTCGACTATGTCAACGGGGGAGAG
  CTCTTCTTCCACCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCT
  GCTGAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTACAGGGATCTG
  AAACCAGAGAACATTCTCTTGGACTGCCAGGGACACGTGGTGCTGACGGATTTTGGCCTC
  TGCAAGGAAGGTGTAGAGCCTGAAGACACCACATCCACATTCTGTGGTACCCCTGAGTAC
  TTGGCACCTGAAGTGCTTCGGAAAGAGCCTTATGATCGAGCAGTGGACTGGTGGTGCTTG
  GGGGCAGTCCTCTACGAGATGCTCCATGGCCTGCCGCCCTTCTACAGCCAAGATGTATCC
  CAGATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCGGACAGTGGCC
  GCCTGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAGGCAGCGGCTGGGCTCCAAA
  GCAGACTTTCTTGAGATTAAGAACCATGTATTCTTCAGCCCCATAAACTGGGATGACCTG
  TACCACAAGAGGCTAACTCCACCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAG
  CATTTTGACCCAGAGTTCACCCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGAC
  ACTGTGGCCAGCAGCTCTGGGGCCTCAAGTGCATTCCTGGGATTTTCTTATGCGCCAGAG
  GATGATGACATCTTGGATTGCTAG
  
  Clone variation with respect to NM_170693.2
  191 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_170693 unedited
  ACCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGACAAGTCAGGATT
  GTGCCTCCAGTCAGAAGGACCATGAAGAGAGAGAAGAGGGCAGAGCCGTGCATGGGGCTGCTCCCCAGGA
  CCTGAGCAGGAACCTGGAGTTTTCAGAGCTGCCTGATCATTGCTACAGAATGAACTCTAGCCCAGCTGGG
  ACCCCAAGTCCACAGCCCTCCAGGGGCCAATGGGAACATCAACCTGGGGGCCTTCAGCCAACCCAGATGC
  GCAGCTCCACGGACTTCGACTTTCCTCAAGGTCATCGGCAAAGGGAACTACGGAAAGGTCCACCGGGCGA
  ACCGCAATTCTGAGGGGGCGGTTCTATGCATGGATGGTCCTACAGAAAAAGTTCAATCTAAAAGAAAAAA
  AAACCAAAGCCCATTCTTGCCGAAACCGCAGTTGTGCTTTGGAGAAAGTGGGGGGCCCCCCTTCCCTCGG
  GGCCCGGCCCAATCCTTTCCAAAACCTGGGAAACCCTTACTTTTGGGCGAAAATGTTTACAGGGGGAAAG
  AGTTTTTTTTCATTGGGAGGGGGCGCCCGGTTTCGGGACCCCGGGCCCAGGTTTCACGCTGCAGGTGGGG
  CCCGGCCCTTTGTATCTGTGTTCCCCCATCATTTTTACGGGTGTTGGAACCAGAAAATTTTTTTTTACAC
  GCGAGGCACTGTGGCTGCCGAGTTTTTTTGCCTCTCAGAAGGAGGTTAACCCCCGAACACCCCACCCTCT
  TTGGGGGCACCCGAGATTTGCGCGCGATGAGTGTCTAGAAGCCACTATATATCCATCTATACGGTGTGCT
  GTGTGTGCGGCCTTCTACAGAGACATAGTGAGCGGACTCTCTGCACATAGATATCGACGATATGAAATAT
  ACGCTCTGACGACA
  
  Kinase domain sequence

  >SC323365 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AYARKSRGRYTTCGCGTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGT
  TTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGACAAGT
  CAGGATTGTGCCTCCAGTCAGAAGGACCATGAAGAGAGAGAAGAGGGCAGAGCCGTGCATGGGGCTGCTC
  CCCAGGACCTGAGCAGGAACCTGGAGTTTTCAGAGCTGCCTGATC
  
• Restriction Sites: Please inquire
• ACCN: NM_170693
• ORF Size: 1104 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_170693.1, NP_733794.1
• RefSeq Size: 1862
• RefSeq ORF: 1104
• Locus ID: 10110
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK), this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) contains an alternate in-frame exon in the 5' coding region, compared to variant 2. Variants 1 and 3 encode the same isoform.