SGK2 (NM_170693) Human Untagged Clone

CAT#: SC323599

SGK2 (untagged)-Kinase deficient mutant (K64M) of Human serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SGK2
• Synonyms: dJ138B7.2; H-SGK2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_170693, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACTCTAGCCCAGCTGGGACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATCAACCTGGGGC
  CTTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTCATCGGCAAAGGGAACTACGG
  GAAGGTCCTACTGGCCAAGCGCAAGTCTGATGGGGCGTTCTATGCAGTGAAGGTACTACAGAAAAAGTCC
  ATCTTAAAGAAGAAAGAGCAGAGCCACATCATGGCAGAGCGCAGTGTGCTTCTGAAGAACGTGCGGCACC
  CCTTCCTCGTGGGCCTGCGCTACTCCTTCCAGACACCTGAGAAGCTCTACTTCGTGCTCGACTATGTCAA
  CGGGGGAGAGCTCTTCTTCCACCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCT
  GCTGAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTACAGGGATCTGAAACCAGAGA
  ACATTCTCTTGGACTGCCAGGGACACGTGGTGCTGACGGATTTTGGCCTCTGCAAGGAAGGTGTAGAGCC
  TGAAGACACCACATCCACATTCTGTGGTACCCCTGAGTACTTGGCACCTGAAGTGCTTCGGAAAGAGCCT
  TATGATCGAGCAGTGGACTGGTGGTGCTTGGGGGCAGTCCTCTACGAGATGCTCCATGGCCTGCCGCCCT
  TCTACAGCCAAGATGTATCCCAGATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCG
  GACAGTGGCCGCCTGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAGGCAGCGGCTGGGCTCCAAA
  GCAGACTTTCTTGAGATTAAGAACCATGTATTCTTCAGCCCCATAAACTGGGATGACCTGTACCACAAGA
  GGCTAACTCCACCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAGCATTTTGACCCAGAGTTCAC
  CCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGACACTGTGGCCAGCAGCTCTGGGGCCTCAAGT
  GCATTCCTGGGATTTTCTTATGCGCCAGAGGATGATGACATCTTGGATTGCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_170693 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCTCCAGTCAGAAG
  GACCATGAAGAGAGAGAAGAGGGCAGAGCCGTGCATGGGGCTGCTCCCCAGGACCTGAGCAGGAACCTGG
  AGTTTTCAGAGCTGCCTGATCATTGCTACAGAATGAACTCTAGCCCAGCTGGGACCCCAAGTCCACAGCC
  CTCCAGGGCCAATGGGAACATCAACCTGGGGCCTTCAGCCAACCCAAATGCCCAGCCCACGGACTTTGGA
  CTTTCCTCAAAGTCATCGGGCAAAAGGGAACTACGGGGAAGGTCCTACTGGCCAAGCGCAGGTCTGATGG
  GGGCGGTTCTTATGCCATGGATGGTCCTACAGAAAAAAGTTCCATCTTAAAGAAAAAAAAACCAGAAGCC
  ACTTCAGGGCAAAAGCGCATGTGTGCTTCTGAGAAAACTTCGGGGCACCCCTTCCTCGGGGGCTGCGCGC
  ATCCCCCTTCAGAAAACCTTGGAGAACCCTTTACTTGTGGTGCTGCACATTGTCACAGCGGGGGAAGAGT
  TTTTTTTTAAATGGAGAGGGGAGGCGCCCGGTTCTGGAAAGCCACGAGCCAGGTTTACCCCCTTCTTAAT
  TTCCAACCCCCCAATGGGATTCTTTGCTCTCCCTACAACTTTTTTCAGGGTCTTTTAAAAGCAAAACACA
  TTTTTTTTTGATACCCAGAGACCCCGGGGGTGTTAACGTTTTTGCGCCCTCCTACGAAGAGGGTGAGACC
  GCAGAAAAAGATCATCTTCTTTGGGTGGTCCCCCATAATCGTGGCCCAATAGCTCTGAGAACACATTAAT
  CATACAACAATGACGTGTGCTTGTGGCGCACCTCCTAACGAATCCTAGACGAGCGCGCTCTCTTACGCCA
  GTATGCGCTACGATGTGATACAT
  
  Kinase domain sequence

  >SC323599 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCTCCAGTCAGAAGGACC
  ATGAAGAGAGAGAAGAGGGCAGAGCCGTGCATGGGGCTGCTCCCCAGGACCTGAGCAGGAACCTGGAGTT
  TTCAGAGCTGCCTGATCATTGCTACAGAATGAACTCTAGCCCAGC
  
• Restriction Sites: Please inquire
• ACCN: NM_170693
• ORF Size: 1104 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_170693.1, NP_733794.1
• RefSeq Size: 1862
• RefSeq ORF: 1104
• Locus ID: 10110
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK), this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (1) contains an alternate in-frame exon in the 5' coding region, compared to variant 2. Variants 1 and 3 encode the same isoform.