CaMKI gamma (CAMK1G) (NM_020439) Human Untagged Clone

CAT#: SC323374

CAMK1G (untagged)-Kinase deficient mutant (K52M) of Human calcium/calmodulin-dependent protein kinase IG (CAMK1G)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAMK1G
• Synonyms: CLICK3; CLICKIII; dJ272L16.1; VWS1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323374 sequence for NM_020439 edited (data generated by NextGen Sequencing)
  ATGGGTCGAAAGGAAGAAGATGACTGCAGTTCCTGGAAGAAACAGACCACCAACATCCGG
  AAAACCTTCATTTTTATGGAAGTGCTGGGATCAGGAGCTTTCTCAGAAGTTTTCCTGGTG
  AAGCAAAGACTGACTGGGAAGCTCTTTGCTCTGATGTGCATCAAGAAGTCACCTGCCTTC
  CGGGACAGCAGCCTGGAGAATGAGATTGCTGTGTTGAAAAAGATCAAGCATGAAAACATT
  GTGACCCTGGAGGACATCTATGAGAGCACCACCCACTACTACCTGGTCATGCAGCTTGTT
  TCTGGTGGGGAGCTCTTTGACCGGATCCTGGAGCGGGGTGTCTACACAGAGAAGGATGCC
  AGTCTGGTGATCCAGCAGGTCTTGTCGGCAGTGAAATACCTACATGAGAATGGCATCGTC
  CACAGAGACTTAAAGCCCGAAAACCTGCTTTACCTTACCCCTGAAGAGAACTCTAAGATC
  ATGATCACTGACTTTGGTCTGTCCAAGATGGAACAGAATGGCATCATGTCCACTGCCTGT
  GGGACCCCAGGCTACGTGGCTCCAGAAGTGCTGGCCCAGAAACCCTACAGCAAGGCTGTG
  GATTGCTGGTCCATCGGCGTCATCACCTACATATTGCTCTGTGGATACCCCCCATTCTAT
  GAAGAAACGGAGTCTAAGCTTTTCGAGAAGATCAAGGAGGGCTACTATGAGTTTGAGTCT
  CCATTCTGGGATGACATTTCTGAGTCAGCCAAGGACTTTATTTGCCACTTGCTTGAGAAG
  GATCCGAACGAGCGGTACACCTGTGAGAAGGCCTTGAGTCATCCCTGGATTGACGGAAAC
  ACAGCCCTCCACCGGGACATCTACCCATCAGTCAGCCTCCAGATCCAGAAGAACTTTGCT
  AAGAGCAAGTGGAGGCAAGCCTTCAACGCAGCAGCTGTGGTGCACCACATGAGGAAGCTA
  CACATGAACCTGCACAGCCCGGGCGTCCGCCCAGAGGTGGAGAACAGGCCGCCTGAAACT
  CAAGCCTCAGAAACCTCTAGACCCAGCTCCCCTGAGATCACCATCACCGAGGCACCTGTC
  CTGGACCACAGTGTAGCACTCCCTGCCCTGACCCAATTACCCTGCCAGCATGGCCGCCGG
  CCCACTGCCCCTGGTGGCAGGTCCCTCAACTGCCTGGTCAATGGCTCCCTCCACATCAGC
  AGCAGCCTGGTGCCCATGCATCAGGGGTCCCTGGCCGCCGGGCCCTGTGGCTGCTGCTCC
  AGCTGCCTGAACATTGGGAGCAAAGGAAAGTCCTCCTACTGCTCTGAGCCCACACTCCTC
  AAAAAGGCCAACAAAAAACAGAACTTCAAGTCGGAGGTCATGGTACCAGTTAAAGCCAGT
  GGCAGCTCCCACTGCCGGGCAGGGCAGACTGGAGTCTGTCTCATTATGTGA
  
  Clone variation with respect to NM_020439.2
  155 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_020439 unedited
  ACCCCCCCGTCCAGCAACGGGCGGTAGGCGCTGTACGGCTGTGGAGGTCTATATAAGCAGAGCTCGTTTA
  GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCTAATC
  CTCACAGACCTGTAGGAGCTGGAGTGGGAGCTCAAGCAGGATTCTTCCCGAGTCCCTGGCATCCTCAGAA
  GCTTCAACTCTGGAGGCAATGGGTCGAAAGGAAAAAAATGACTGCGTTTCCTGGAAAAAACAAACCACCC
  TCATTATTGTTAACCTTGATTTTTATGGCCTGCTAGAATCAGGAAAATACATCAACTGTACTTTAAGAAG
  AGAAGAAAGGGTCCGGAAGGGGTAGGGAGAAACGCACAGAAGAAAAAAAAAAACAAAACCGAAAGATGGA
  AAGCAGCCCAAGGGGGGGGGGGGGGCCCTGAACA
  
  Kinase domain sequence

  >SC323374 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCATGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCTAATCCTCACAGACCT
  GTAGGAGCTGGAGTGGGAGCTCAAGCAGGATTCTTCCCGAGTCCCTGGCATCCTCAGAAGCTTCAACTCT
  GGAGGCAATGGGTCGAAAGGAAGAAGATGACTGCAGTTCCTGGAA
  
• Restriction Sites: Please inquire
• ACCN: NM_020439
• ORF Size: 1431 bp
• Insert Size: 2780
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_020439.2, NP_065172.1
• RefSeq Size: 2507
• RefSeq ORF: 1431
• Locus ID: 57172
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a protein similar to calcium/calmodulin dependent protein kinase, however, its exact function is not known. [provided by RefSeq, Jul 2008]