SGK3 (NM_013257) Human Untagged Clone

CAT#: SC323383

SGK3 (untagged)-Kinase deficient mutant (K191M) of Human serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SGK3
• Synonyms: CISK; SGK2; SGKL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013257, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAAAGAGATCACACCATGGACTACAAGGAAAGCTGCCCAAGTGTAAGCATTCCCAGCTCCGATGAAC
  ACAGAGAGAAAAAGAAGAGGTTTACTGTTTATAAAGTTCTGGTTTCAGTGGGAAGAAGTGAATGGTTTGT
  CTTCAGGAGATATGCAGAGTTTGATAAACTTTATAACACTTTAAAAAAACAGTTTCCTGCTATGGCCCTG
  AAGATTCCTGCCAAGAGAATATTTGGTGATAATTTTGATCCAGATTTTATTAAACAAAGACGAGCAGGAC
  TAAACGAATTCATTCAGAACCTAGTTAGGTATCCAGAACTTTATAACCATCCAGATGTCAGAGCATTCCT
  TCAAATGGACAGTCCAAAACACCAGTCAGATCCATCTGAAGATGAGGATGAAAGAAGTTCTCAGAAGCTA
  CACTCTACCTCACAGAACATCAACCTGGGACCGTCTGGAAATCCTCATGCCAAACCAACTGACTTTGATT
  TCTTAAAAGTTATTGGAAAAGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACTGGATGGAAAATT
  TTATGCTGTCAAAGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATATTATGGCTGAA
  CGTAATGTGCTCTTGAAAAATGTGAAACATCCGTTTTTGGTTGGATTGCATTATTCCTTCCAAACAACTG
  AAAAGCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTTTTTTTCCACTTACAAAGAGAACGGTC
  CTTTCCTGAGCACAGAGCTAGGTTTTACGCTGCTGAAATTGCTAGTGCATTGGGTTACTTACATTCCATC
  AAAATAGTATACAGAGACTTGAAACCAGAAAATATTCTTTTGGATTCAGTAGGACATGTTGTCTTAACAG
  ATTTTGGGCTTTGTAAAGAAGGAATTGCTATTTCTGACACCACTACCACATTTTGTGGGACACCAGAGTA
  TCTTGCACCTGAAGTAATTAGAAAACAGCCCTATGACAATACTGTAGATTGGTGGTGCCTTGGGGCTGTT
  CTGTATGAAATGCTGTATGGATTGCCTCCTTTTTATTGCCGAGATGTTGCTGAAATGTATGACAATATCC
  TTCACAAACCCCTAAGTTTGAGGCCAGGAGTGAGTCTTACAGCCTGGTCCATTCTGGAAGAACTCCTAGA
  AAAAGACAGGCAAAATCGACTTGGTGCCAAGGAAGACTTTCTTGAAATTCAGAATCATCCTTTTTTTGAA
  TCACTCAGCTGGGCTGACCTTGTACAAAAGAAGATTCCACCACCATTTAATCCTAATGTGGCTGGACCAG
  ATGATATCAGAAACTTTGACACAGCATTTACAGAAGAAACAGTTCCATATTCTGTGTGTGTATCTTCTGA
  CTATTCTATAGTGAATGCCAGTGTATTGGAGGCAGATGATGCATTCGTTGGTTTCTCTTATGCACCTCCT
  TCAGAAGACTTATTTTTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_013257 unedited
  ACCGCCCGCTCCAGCAAAGGGCGGTAGGCGCGTACGGCGGGAGGTTCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGGCCGAGT
  GTGGCGCAGCAGTGGCGGCGCTTCCCATTCGCCATGCGCCGGGGGTGGGTGCCCGAAGGTTGCATGATGG
  AATTTGAACATTACTTCAAGAGGTTTTGTATTTTGGATTAGTTAATTGGGTTTGTCCTCTGCTGACTGTT
  TCTTCGGATGCATTTTTTGGTGTGCTCTTGAGGATTAAATGCAAAGAGATCACACCAGGACAGATGAAGC
  GCCAAGTGTAGCTTCCAACCCCATGAAACGAGAAAAAAAAGAAGGGACTGGGAAAGTTTGTGGATTGAAA
  AGTAAAGGTTTGTCCTTCCGGGAAAATCCCAAATTTTAAAAAATTTTAAAACCCTTTTAAAAAAAAATTT
  TTCCGGGCTAGGCCCCGGAAAAATTCCTGCCAAAAAAAAAATTTGGGGGAAAATTTTTTTTCCAAATTTT
  TTTAAAAAAAAAAAAAACGGGATAAACAAATTTTTTTTTAAACCCATTTTGGGTCCCAAAATTTTTTCCC
  CCCCAAGTTTAAAGATTTCTTTCAATGGGGGGGCAAAAACACAATATATACTCCGTGAAGAAAGAGAAAA
  AAAAAATTTTAGAGGACCCCCACCACCCCAAAACAAAAGCGAGCGCCCGGGGA
  
  Kinase domain sequence

  >SC323383 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TWCTAAGTATTGGAAAGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACTGGATGGAAAATTTTAT
  GCTGTCATGGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATATTATGGCTGAACGTA
  ATGTGCTCTTGAAAAATGTGAAACATCCGTTTTTGGTTGGATTGCATTATTCCTTCCAAACAACTGAAAA
  GCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTTTTTTT
  
• Restriction Sites: Please inquire
• ACCN: NM_013257
• ORF Size: 1491 bp
• Insert Size: 2680
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_013257.3, NP_037389.4
• RefSeq Size: 4155
• RefSeq ORF: 1491
• Locus ID: 23678
• Domains: pkinase, S_TK_X, TyrKc, PX, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 3 encode the same isoform.