STK32A (NM_145001) Human Untagged Clone

CAT#: SC323391

STK32A (untagged)-Kinase deficient mutant (K52M) of Human serine/threonine kinase 32A (STK32A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK32A
• Synonyms: YANK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145001, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAGCGAACACTTCAAGAAAACCACCAGTGTTTGATGAAAATGAAGATGTCAACTTTGACCACTTTG
  AAATTTTGCGAGCCATTGGGAAAGGCAGTTTTGGGAAGGTCTGCATTGTACAGAAGAATGATACCAAGAA
  GATGTACGCAATGAAGTACATGAATAAACAAAAGTGCGTGGAGCGCAATGAAGTGAGAAATGTCTTCAAG
  GAACTCCAGATCATGCAGGGTCTGGAGCACCCTTTCCTGGTTAATTTGTGGTATTCCTTCCAAGATGAGG
  AAGACATGTTCATGGTGGTGGACCTCCTGCTGGGTGGAGACCTGCGTTATCACCTGCAACAGAACGTCCA
  CTTCAAGGAAGAAACAGTGAAGCTCTTCATCTGTGAGCTGGTCATGGCCCTGGACTACCTGCAGAACCAG
  CGCATCATTCACAGGGATATGAAGCCTGACAATATTTTACTTGACGAACATGATACCTGGCTCTCCTACA
  AGTCCCACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_145001 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCTTGCTCTTGGAGT
  TCTTCTCTTAGTCCCTGTTCCCTGGATGAAAGCATCGCTCCGAGCCTCATGGGAGGAATGAAGGAAGAAT
  CGAGACTAGATATCCAACTAAGGCTTCGGGACATGTTTTGAGCGAAGATGGGTGTTTCTGCCCGGATAGT
  ATAAATCGAGGATCCAGGTCTGGGCAGATTCAACCATGGGAGCGAACACTTTCAAGAAAAACCACCAGTT
  GTTTGATGAAAAATGAAAGATGTCAACTTTTGACCACTTTTGAATTTTTGCCGAAGCATTTGGGGAAAAG
  GCAGTTTTTGGGGAAAGGTCTGCATTTGTCACAGAAAAAATGAATACCCAAGAAGAATGTACCGCCATTG
  AAGTGTCACTTGAATTAAACAAAAGTTGGCGTGGAGCCCAATGGAAGTTAGAAAAATGTCTCCAAGAAAC
  TCTCCGAAATCATGCAGGGTCTGGACCACCCTTTCCCGGGTAAATTTGGGATATCCCTTCCGAAAAGAGG
  GAGGACAGTGTCCATGGGGGGGGCCCCTCGTGTTGGGGGAACCCGGCTTTTACCCCTGACACGAAGCGTC
  CATTCAGAGAAACAGTTGGACTCTCTACTTTTTGGTGTGCTAGGGCCCTTGGCTATTCTCGAAACCAGCC
  ATCATTTCAGGATTTGAAGCCTCACCAATTAATTGACGAAATGGCCGTCCACTTACAGATTACATGTGCG
  AGTGCCAGAACCAGATTACACTGTGGCAGCGTCATGACCTGAGTGACTCCGAAGACGCATCTCTGCCAGG
  TTCTGTAGCCATAATCGTGAGCGCAA
  
  Kinase domain sequence

  >SC323391 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCTTGCTCTTGGAGTTCTTC
  TCTTAGTCCCTGTTCCCTGGATGAAAGCATCGCTCCGAGCCTCATGGGAGGAATGAAGGAAGAATCGAGA
  CTAGATATCCAACTAAGGCTTCGGGACATGTTTTGAGCGAAGATG
  
• Restriction Sites: Please inquire
• ACCN: NM_145001
• ORF Size: 501 bp
• Insert Size: 4000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_145001.1, NP_659438.1
• RefSeq Size: 827
• RefSeq ORF: 501
• Locus ID: 202374
• Protein Families: Druggable Genome, Protein Kinase