SNRK (NM_017719) Human Untagged Clone

CAT#: SC323405

SNRK (untagged)-Kinase deficient mutant (K45M) of Human SNF related kinase (SNRK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNRK
• Synonyms: HSNFRK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323405 sequence for NM_017719 edited (data generated by NextGen Sequencing)
  ATGGCAGGATTTAAGCGAGGGTATGATGGAAAGATTGCTGGATTATATGATCTGGATAAA
  ACCTTGGGTCGAGGCCATTTTGCCGTGGTTAAACTTGCCAGGCATGTCTTTACGGGTGAA
  AAGGTGGCAGTAATGGTTATTGACAAGACAAAACTGGACACTCTAGCTACTGGTCATCTT
  TTCCAGGAAGTGAGATGCATGAAACTAGTGCAGCATCCTAACATCGTCCGCCTTTATGAA
  GTTATTGACACCCAGACCAAACTATATCTTATTCTAGAACTTGGGGATGGAGGAGATATG
  TTTGATTATATAATGAAACATGAGGAGGGTCTTAATGAAGACTTGGCCAAGAAGTATTTT
  GCTCAGATAGTTCATGCTATATCTTATTGCCATAAACTCCATGTGGTTCACAGAGACTTA
  AAACCAGAGAATGTAGTCTTCTTTGAAAAACAAGGTCTTGTAAAGTTGACAGACTTTGGG
  TTCAGCAACAAATTTCAACCAGGGAAGAAGCTCACTACAAGCTGTGGATCTCTTGCATAT
  TCCGCTCCAGAAATTCTGCTTGGTGATGAGTATGATGCACCTGCAGTAGATATTTGGAGT
  CTGGGAGTGATCCTTTTCATGTTGGTGTGTGGGCAGCCGCCCTTTCAAGAAGCCAATGAC
  AGTGAAACACTGACAATGATCATGGATTGCAAATATACAGTACCATCCCATGTGTCTAAA
  GAGTGTAAAGACCTAATCACACGGATGCTACAGAGAGATCCCAAGAGAAGGGCTTCTTTA
  GAAGAGATTGAAAATCATCCTTGGCTTCAGGGAGTGGACCCTTCACCAGCTACAAAGTAT
  AACATTCCCCTTGTGTCATACAAAAATCTCTCGGAAGAGGAGCACAACAGCATCATTCAG
  CGCATAGTGCTTGGGGACATAGCGGATCGAGACGCCATTGTAGAAGCCCTGGAAACCAAC
  AGGTATAACCATATCACAGCCACATACTTCCTGCTGGCTGAAAGGATCCTGAGAGAAAAG
  CAAGAGAAAGAAATACAGACCAGATCTGCAAGCCCGAGCAATATCAAGGCCCAGTTTAGG
  CAGTCATGGCCAACCAAAATTGATGTACCCCAGGACCTTGAGGATGACCTCACGGCCACT
  CCTTTGTCCCACGCGACTGTCCCTCAGTCTCCTGCTCGGGCTGCTGACAGTGTCCTCAAT
  GGCCACAGGAGCAAAGGCCTGTGTGACTCAGCTAAGAAAGATGACCTCCCTGAGTTGGCT
  GGACCAGCACTCTCTACGGTGCCACCCGCAAGCTTAAAACCCACAGCCAGTGGGCGGAAG
  TGTCTGTTCAGGGTGGAAGAAGATGAAGAGGAAGATGAGGAGGACAAGAAACCCATGTCC
  CTCTCAACACAAGTGGTTTTGCGCCGGAAGCCATCTGTAACCAACCGCCTGACATCCAGG
  AAGAGTGCGCCCGTCCTCAACCAGATCTTTGAGGAAGGGGAATCTGACGATGAGTTTGAC
  ATGGATGAGAATCTGCCTCCCAAGTTGAGCAGGTTAAAGATGAATATAGCTTCTCCAGGT
  ACAGTTCACAAACGCTACCACCGGAGGAAAAGTCAGGGCCGGGGCTCCAGCTGCAGTAGT
  TCGGAGACCAGTGATGATGATTCTGAAAGCCGGCGGCGGCTCGATAAAGATAGCGGGTTC
  ACCTACTCCTGGCACCGACGGGATAGCAGCGAGGGGCCCCCTGGCAGTGAGGGGGATGGC
  GGGGGCCAGAGCAAGCCAAGCAATGCCAGTGGAGGGGTGGACAAGGCCAGCCCCAGTGAG
  AACAATGCTGGTGGGGGCAGTCCCTCCAGCGGCTCGGGTGGCAACCCCACCAATACATCG
  GGTACCACACGCCGCTGTGCCGGCCCCAGCAACTCCATGCAGCTGGCCTCTCGCAGTGCT
  GGGGAGCTCGTTGAGAGCCTCAAACTCATGAGCCTCTGCCTCGGCTCCCAGCTTCATGGG
  AGCACCAAGTACATTATTGATCCACAGAATGGCTTGTCATTTTCCAGTGTGAAAGTCCAA
  GAGAAATCTACGTGGAAAATGTGCATTAGCTCCACAGGGAATGCAGGGCAGGTCCCTGCA
  GTGGGCGGCATAAAGTTTTTCTCTGACCACATGGCAGATACCACCACTGAATTGGAACGG
  ATAAAGAGCAAGAACCTGAAAAATAACGTGCTGCAGCTACCTCTGTGCGAAAAGACCATC
  TCTGTGAACATCCAGCGGAACCCTAAGGAGGGGCTGCTGTGCGCATCCAGCCCAGCCAGC
  TGTTGCCATGTCATCTGA
  
  Clone variation with respect to NM_017719.4
  134 a=>t;135 a=>g;906 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_017719 unedited
  CCCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGAGTGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGACATTGAAAAT
  GAATTTTTTGTATTCACCAGATATTCTTATATGAGAAGATCTATTTTAAACAGTCTAAATATTTTTTCTT
  CTGTTGGACCAGCATGGCAGGATTTAAGCGAGGGTATGATGGAAAGATTGCTGGATTATATGATCTGGAT
  AAAACCTTGGGTCGAGGCCATTTTTGCCGTGGTTAAACTTGCCAGGCATGTCTTTACGGGGTGAAAGGTG
  TGTCGTGTAATGGTTATTGACAAGACACAAAACTGGACACTTCTACCTACTTGTTCATCCTTTTCCAGGA
  GTGGAGAAGGCTGGGAAACTATTGCCACCATTCCTAACTCCTTCCCGCCTTTTTAAATTTATTGACCCCC
  GAACCAACCTATTTCCTAATTCCAAAAACCTGGGGGAGGGAGGAAATTTTTTTTGTTTTTAAATGGAACC
  ATGGGAAAGGGTCTAATAAAAAATTGGGCAAAAAAGTTTTTTGCCCAAAAAGCCAGGCCTAACCCAAAGC
  GCCTAAACCCCGTGGGTTTCACAAAACCTAAACCCAGAAAGTGTTCTCTTTTTTAAAAAAGGGTTTTGAA
  ATTTCACAAATTTGGGTTCCCCCCATTTTCCCCCGGGGAAAACCCCAAAACCTGGGACTCTTTGATTTCC
  CCCCAAAATTTGCTGGGGAGAAATGAATGCCCCCCCGAAATTTGAGTCTGGAGTGTGCCCTTCTTGTGGT
  GGGAGCACCTTTTGAGACCGAGCGGAAACGCGAGATATTGTGTGGCATCAGGCCCCCGCGTGTGTAGTGT
  GAGACCTCCCCCGGCCTCCCCAAT
  
  Kinase domain sequence

  >SC323405 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CKATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGACATTGAAAATGAATTTT
  TTGTATTCACCAGATATTCTTATATGAGAAGATCTATTTTAAACAGTCTAAATATTTTTTCTTCTGTTGG
  ACCAGCATGGCAGGATTTAAGCGAGGGTATGATGGAAAGATTGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_017719
• ORF Size: 2298 bp
• Insert Size: 6220
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_017719.3, NP_060189.2
• RefSeq Size: 5118
• RefSeq ORF: 2298
• Locus ID: 54861
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: SNRK is a member of the sucrose nonfermenting (SNF)-related kinase family of serine/threonine kinases (Kertesz et al., 2002 [PubMed 12234663]). [supplied by OMIM, Apr 2009]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same isoform (1).