TLK2 (NM_006852) Human Untagged Clone

CAT#: SC323426

TLK2 (untagged)-Kinase deficient mutant (K491M) of Human tousled-like kinase 2 (TLK2), transcript variant A


  "NM_006852" in other vectors (8)

Reconstitution Protocol

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    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TLK2
Synonyms HsHPK; MRD57; PKU-ALPHA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_006852, the custom clone sequence may differ by one or more nucleotides


ATGATGGAAGAATTGCATAGCCTGGACCCACGACGGCAGGAATTATTGGAGGCCAGGTTTACTGGAGTAG
GTGTTAGTAAGGGACCACTTAATAGTGAGTCTTCCAACCAGAGCTTGTGCAGCGTCGGATCCTTGAGTGA
TAAAGAAGTAGAGACTCCCGAGAAAAAGCAGAATGACCAGCGAAATCGGAAAAGAAAAGCTGAACCATAT
GAAACTAGCCAAGGGAAAGGCACTCCTAGGGGACATAAAATTAGTGATTACTTTGAGTTTGCTGGGGGAA
GCGCGCCAGGAACCAGCCCTGGCAGAAGTGTTCCACCAGTTGCACGATCCTCACCGCAACATTCCTTATC
CAATCCCTTACCGCGACGAGTAGAACAGCCCCTCTATGGTTTAGATGGCAGTGCTGCAAAGGAGGCAACG
GAGGAGCAGTCTGCTCTGCCAACCCTCATGTCAGTGATGCTAGCAAAACCTCGGCTTGACACAGAGCAGC
TGGCGCAAAGGGGAGCTGGCCTCTGCTTCACTTTTGTTTCAGCTCAGCAAAACAGTCCCTCATCTACGGG
ATCTGGCAACACAGAGCATTCCTGCAGCTCCCAAAAACAGATCTCCATCCAGCACAGACAGACCCAGTCC
GACCTCACAATAGAAAAAATATCTGCACTAGAAAACAGTAAGAATTCTGACTTAGAGAAGAAGGAGGGAA
GAATAGATGATTTATTAAGAGCCAACTGTGATTTGAGACGGCAGATTGATGAACAGCAAAAGATGCTAGA
GAAATACAAGGAACGATTAAATAGATGTGTGACAATGAGCAAGAAACTCCTTATAGAAAAGTCAAAACAA
GAGAAGATGGCGTGTAGAGATAAGAGCATGCAAGACCGCTTGAGACTGGGCCACTTTACTACTGTCCGAC
ACGGAGCCTCATTTACTGAACAGTGGACAGATGGTTATGCTTTTCAGAATCTTATCAAGCAACAGGAAAG
GATAAATTCACAGAGGGAAGAGATAGAAAGACAACGGAAAATGTTAGCAAAGCGGAAACCTCCTGCCATG
GGTCAGGCCCCTCCTGCAACCAATGAGCAGAAACAGCGGAAAAGCAAGACCAATGGAGCTGAAAATGAAA
CGTTAACGTTAGCAGAATACCATGAACAAGAAGAAATCTTCAAACTCAGATTAGGTCATCTTAAAAAGGA
GGAAGCAGAGATCCAGGCAGAGCTGGAGAGACTAGAAAGGGTTAGAAATCTACATATCAGGGAACTAAAA
AGGATACATAATGAAGATAATTCACAATTTAAAGATCATCCAACGCTAAATGACAGATATTTGTTGTTAC
ATCTTTTGGGTAGAGGAGGTTTCAGTGAAGTTTACAAGGCATTTGATCTAACAGAGCAAAGATACGTAGC
TGTGAAAATTCACCAGTTAAATAAAAACTGGAGAGATGAGAAAAAGGAGAATTACCACAAGCATGCATGT
AGGGAATACCGGATTCATAAAGAGCTGGATCATCCCAGAATAGTTAAGCTGTATGATTACTTTTCACTGG
ATACTGACTCGTTTTGTACAGTATTAGAATACTGTGAGGGAAATGATCTGGACTTCTACCTGAAACAGCA
CAAATTAATGTCGGAGAAAGAGGCCCGGTCCATTATCATGCAGATTGTGAATGCTTTAAAGTACTTAAAT
GAAATAAAACCTCCCATCATACACTATGACCTCAAACCAGGTAATATTCTTTTAGTAAATGGTACAGCGT
GTGGAGAGATAAAAATTACAGATTTTGGTCTTTCGAAGATCATGGATGATGATAGCTACAATTCAGTGGA
TGGCATGGAGCTAACATCACAAGGTGCTGGTACTTATTGGTATTTACCACCAGAGTGTTTTGTGGTTGGG
AAAGAACCACCAAAGATCTCAAATAAAGTTGATGTGTGGTCGGTGGGTGTGATCTTCTATCAGTGTCTTT
ATGGAAGGAAGCCTTTTGGCCATAACCAGTCTCAGCAAGACATCCTACAAGAGAATACGATTCTTAAAGC
TACTGAAGTGCAGTTCCCGCCAAAGCCAGTAGTAACACCTGAAGCAAAGGCGTTTATTCGACGATGCTTG
GCCTACCGAAAGGAGGACCGCATTGATGTCCAGCAGCTGGCCTGTGATCCCTACTTGTTGCCTCACATCC
GAAAGTCAGTCTCTACAAGTAGCCCTGCTGGAGCTGCTATTGCATCAACCTCTGGGGCGTCCAATAACAG
TTCTTCTAATTGA


>OriGene 5' read for mutant NM_006852 unedited
CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAATTGCATAGCCT
GGACCCACGACGGCAGGAATTATTGGAGGCCAGGTTTACTGGAGTAGGTGTTAGTAAGGGACCACTTAAT
AGTGAGTCTTCCAACCAGAGCTTGTGCAGCGTCGGATCCTTGAGTGATAAAGAAGTAGAGACTCCCGAGA
AAAAGCAGAATGACCAGCGAAATCGGAAAAGAAAAGCTGAACCATATGAAACTAGCCAAGGGAAAGGCAC
TCCTAGGGGACATAAAATTAGTGATTACTTTTGAGCGACGAGTAGAACAGCCCCTCTATGGTTTAGATGG
CAGTGCTGCAAAGGAGGCAACGGAGGAGCAGTCTGCTCTGCCAACCCTCATGTCAGTGATGCTAGCAAAC
CTCGCTTGACACAGAGCAGCTGCGCAAAGGGGAGCTGCCTCTGCTTCACTTTTGTTTCAGCTCAGCAAAA
CAGTCCCTCATCTACGGATTCTGCAACACAGGAGCATCTGCAGCTCCAAAAAACAGAATTCCTCATCAGC
ACAGACGGACCCAGTCGGACTTCAATGGAAAAAAATACTCTGCACCATAGAAACAGTAAGAATCCGGACT
AGAGAAAGGAGGGAAGAATACTGTAGATTTTATAGAGCCCACTGATTGAACCGCGCAATGTTGTAGACCC
GCAAATGTCCTAGAAATTCACTAGACACGTATTATATATGTGTGACATGGACAGAATCTCTTAAGAAGTC
CCACCAGAGATGGCGTGGAATAGACTGGAAGTCGATGGACTTGCATTTACTTGGCACGATCCTATCGCAC
ATGGCCAAGTAGAGC
>SC323426 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CMSGCTAATGACAGATATTTGTTGTTACATCTTTTGGGTAGAGGAGGTTTCAGTGAAGTTTACAAGGCAT
TTGATCTAACAGAGCAAAGATACGTAGCTGTGATGATTCACCAGTTAAATAAAAACTGGAGAGATGAGAA
AAAGGAGAATTACCACAAGCATGCATGTAGGGAATACCGGATTCATAAAGAGCTGGATCATCCCAGAATA
GTTAAGCTGTATGATTACTTTTCACTGGATACTGACTCGTTTTGT
Restriction Sites Please inquire     
ACCN NM_006852
ORF Size 2253 bp
Insert Size 3270
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Reference Data
RefSeq NM_006852.2, NP_006843.2
RefSeq Size 3616
RefSeq ORF 2253
Locus ID 11011
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Transcript Variant: This variant (A) lacks an alternate in-frame exon in the 3' coding region compared to variant C. The encoded protein (isoform A) is shorter than isoform C.

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