RSK3 (RPS6KA2) (NM_021135) Human Untagged Clone

CAT#: SC323446

RPS6KA2 (untagged)-Kinase deficient mutant (K91M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 2 (RPS6KA2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS6KA2
• Synonyms: HU-2; MAPKAPK1C; p90-RSK3; p90RSK2; pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323446 sequence for NM_021135 edited (data generated by NextGen Sequencing)
  ATGGACCTGAGCATGAAGAAGTTCGCCGTGCGCAGGTTCTTCTCTGTGTACCTGCGCAGG
  AAGTCGCGCTCCAAGAGCTCCAGCCTGAGCCGGCTCGAGGAAGAAGGTGTCGTGAAGGAG
  ATAGACATCAGCCATCATGTGAAGGAGGGCTTTGAGAAGGCAGATCCTTCCCAGTTTGAG
  CTGCTGAAGGTTTTAGGACAAGGATCCTATGGAAAGGTGTTCCTGGTGAGGAAGGTGAAG
  GGGTCCGACGCTGGGCAGCTCTACGCCATGATGGTCCTTAAGAAAGCCACCCTAAAAGTT
  CGGGACCGAGTGAGATCGAAGATGGAGAGAGACATCTTGGCAGAAGTGAATCACCCCTTC
  ATTGTGAAGCTTCATTATGCCTTTCAGACGGAAGGAAAGCTCTACCTGATCCTGGACTTC
  CTGCGGGGAGGGGACCTCTTCACCCGGCTCTCCAAAGAGGTCATGTTCACGGAGGAGGAT
  GTCAAGTTCTACCTGGCTGAGCTGGCCTTGGCTTTAGACCATCTCCACAGCCTGGGGATC
  ATCTACAGAGATCTGAAGCCTGAGAACATCCTCCTGGATGAAGAGGGGCACATTAAGATC
  ACAGATTTCGGCCTGAGTAAGGAGGCCATTGACCACGACAAGAGAGCGTACTCCTTCTGC
  GGGACGATCGAGTACATGGCGCCCGAGGTGGTGAACCGGCGAGGACACACGCAGAGTGCC
  GACTGGTGGTCCTTCGGCGTGCTCATGTTTGAGATGCTCACGGGGTCCCTGCCGTTCCAG
  GGGAAGGACAGGAAGGAGACCATGGCTCTCATCCTCAAAGCCAAGCTGGGGATGCCGCAG
  TTCCTCAGTGGGGAGGCACAGAGTTTGCTGCGAGCTCTCTTCAAACGGAACCCCTGCAAC
  CGGCTGGGTGCTGGCATTGACGGAGTGGAGGAAATTAAGCGCCATCCCTTCTTTGTGACC
  ATAGACTGGAACACGCTGTACCGGAAGGAGATCAAGCCACCGTTCAAACCAGCAGTGGGC
  AGGCCTGAGGACACCTTCCACTTTGACCCCGAGTTCACAGCGCGGACGCCCACAGACTCT
  CCTGGCGTCCCCCCGAGTGCAAACGCTCATCACCTGTTTAGAGGATTCAGCTTTGTGGCC
  TCAAGCCTGATCCAGGAGCCCTCACAGCAAGATCTGCACAAAGTCCCAGTTCACCCAATC
  GTGCAGCAGTTACACGGGAACAACATCCACTTCACCGATGGCTACGAGATCAAGGAGGAC
  ATCGGGGTGGGCTCCTACTCAGTGTGCAAGCGATGTGTGCATAAAGCCACAGACACCGAG
  TATGCCGTGAAGATCATTGATAAGAGCAAGAGAGACCCCTCGGAAGAGATTGAGATCCTC
  CTGCGGTACGGCCAGCACCCGAACATCATCACCCTCAAGGATGTCTATGATGATGGCAAG
  TTTGTGTACCTGGTAATGGAGCTGATGCGTGGTGGGGAGCTCCTGGACCGCATCCTCCGG
  CAGAGATACTTCTCGGAGCGCGAAGCCAGTGACGTCCTGTGCACCATCACCAAGACCATG
  GACTACCTCCATTCCCAGGGGGTTGTTCATCGAGACCTGAAGCCGAGTAACATCCTGTAC
  AGGGATGAGTCGGGGAGCCCAGAATCCATCCGAGTCTGCGACTTCGGCTTTGCCAAGCAG
  CTGCGCGCGGGGAACGGGCTGCTCATGACACCCTGCTACACGGCCAATTTCGTGGCCCCG
  GAGGTCCTGAAGCGTCAAGGCTATGATGCGGCGTGTGACATCTGGAGTTTGGGGATCCTG
  TTGTACACCATGCTGGCAGGATTTACCCCTTTTGCAAATGGGCCAGACGATACCCCTGAG
  GAGATTCTGGCGCGGATCGGCAGTGGGAAGTATGCCCTTTCTGGGGGAAACTGGGACTCG
  ATATCTGACGCAGCTAAAGACGTCGTGTCCAAGATGCTCCACGTGGACCCTCATCAGCGC
  CTGACGGCGATGCAAGTGCTCAAACACCCGTGGGTGGTCAACAGAGAGTACCTGTCCCCA
  AACCAGCTCAGCCGACAGGACGTGCACCTGGTGAAGGGCGCGATGGCCGCCACCTACTTT
  GCTCTAAACAGAACACCTCAGGCCCCGCGGCTGGAGCCCGTGCTGTCGTCCAACCTGGCT
  CAGCGCAGAGGCATGAAGAGACTCACGTCCACGCGGTTGTAG
  
  Clone variation with respect to NM_021135.4
  108 c=>t;272 a=>t;2148 a=>g;2197 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_021135 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGGCGCGGCCTGC
  CCTTTGTGACCGCAGCTCGCGCCCCACGCCCCGCGCCCATGGCCGCCGTGCCGGGCTCCCTGGCCACGCG
  TGCCCGCCCGCGGACCTGAGCCCCGCGCCTGGGATGCCGGGGATGCGCGTCCCCCGGCCCTGCGGCTGCT
  CCGGGCTGGGCGCGGGGCGATGGACCTGAGCATGAAGAAGTTCGCCGTGCGCAGGTTCTTCTCTGTGTAC
  CTGCGCAGGAAGTCGCGCTCCCAAGAGCTCCAGCCTGAGCCCGGCTCGAGGAAGAAGGTGTCGTGAAGGA
  GATAGACATCAGCCCATCATGGTGAAGGAGGGCTTTGGAGAAGGCAGATCCTTCCCAGTTGAGCTGCTGA
  AGGTTTTAGGAACAGGATCCCATGGAAAGGTGTTCCTGGTGAGGAAGGTGAAGGGTCCAACCTGGCAACC
  TAACCCATGAGGTCCTAGAAAGCCCCTTAAATTCGGGACCAATAAATCCACATGAAA
  
  Kinase domain sequence

  >SC323446 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TRTTAAGCAGWCTATGGAAGGTGTTCCTGGTGAGGAAGGTGAAGGGGTCCGACGCTGGGCAGCTCTACGC
  CATGATGGTCCTTAAGAAAGCCACCCTAAAAGTTCGGGACCGAGTGAGATCGAAGATGGAGAGAGACATC
  TTGGCAGAAGTGAATCACCCCTTCATTGTGAAGCTTCATTATGCCTTTCAGACGGAAGGAAAGCTCTACC
  TGATCCTGGACTTCCTGCGGGGAGGGGACCTCTTCACCCGGCTCT
  
• Restriction Sites: Please inquire
• ACCN: NM_021135
• Insert Size: 4310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021135.4, NP_066958.2
• RefSeq Size: 5817 bp
• RefSeq ORF: 2202 bp
• Locus ID: 6196
• Cytogenetics: 6q27
• Domains: pkinase, S_TK_X, TyrKc, PDZ, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: 'This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternative splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.