RSK3 (RPS6KA2) (NM_021135) Human Untagged Clone

CAT#: SC323643

RPS6KA2 (untagged)-Kinase deficient mutant (K444M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 2 (RPS6KA2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS6KA2
• Synonyms: HU-2; MAPKAPK1C; p90-RSK3; p90RSK2; pp90RSK3; RSK; RSK3; S6K-alpha; S6K-alpha2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021135, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACCTGAGCATGAAGAAGTTCGCCGTGCGCAGGTTCTTCTCTGTGTACCTGCGCAGGAAGTCGCGCT
  CCAAGAGCTCCAGCCTGAGCCGGCTCGAGGAAGAAGGCGTCGTGAAGGAGATAGACATCAGCCATCATGT
  GAAGGAGGGCTTTGAGAAGGCAGATCCTTCCCAGTTTGAGCTGCTGAAGGTTTTAGGACAAGGATCCTAT
  GGAAAGGTGTTCCTGGTGAGGAAGGTGAAGGGGTCCGACGCTGGGCAGCTCTACGCCATGAAGGTCCTTA
  AGAAAGCCACCCTAAAAGTTCGGGACCGAGTGAGATCGAAGATGGAGAGAGACATCTTGGCAGAAGTGAA
  TCACCCCTTCATTGTGAAGCTTCATTATGCCTTTCAGACGGAAGGAAAGCTCTACCTGATCCTGGACTTC
  CTGCGGGGAGGGGACCTCTTCACCCGGCTCTCCAAAGAGGTCATGTTCACGGAGGAGGATGTCAAGTTCT
  ACCTGGCTGAGCTGGCCTTGGCTTTAGACCATCTCCACAGCCTGGGGATCATCTACAGAGATCTGAAGCC
  TGAGAACATCCTCCTGGATGAAGAGGGGCACATTAAGATCACAGATTTCGGCCTGAGTAAGGAGGCCATT
  GACCACGACAAGAGAGCGTACTCCTTCTGCGGGACGATCGAGTACATGGCGCCCGAGGTGGTGAACCGGC
  GAGGACACACGCAGAGTGCCGACTGGTGGTCCTTCGGCGTGCTCATGTTTGAGATGCTCACGGGGTCCCT
  GCCGTTCCAGGGGAAGGACAGGAAGGAGACCATGGCTCTCATCCTCAAAGCCAAGCTGGGGATGCCGCAG
  TTCCTCAGTGGGGAGGCACAGAGTTTGCTGCGAGCTCTCTTCAAACGGAACCCCTGCAACCGGCTGGGTG
  CTGGCATTGACGGAGTGGAGGAAATTAAGCGCCATCCCTTCTTTGTGACCATAGACTGGAACACGCTGTA
  CCGGAAGGAGATCAAGCCACCGTTCAAACCAGCAGTGGGCAGGCCTGAGGACACCTTCCACTTTGACCCC
  GAGTTCACAGCGCGGACGCCCACAGACTCTCCTGGCGTCCCCCCGAGTGCAAACGCTCATCACCTGTTTA
  GAGGATTCAGCTTTGTGGCCTCAAGCCTGATCCAGGAGCCCTCACAGCAAGATCTGCACAAAGTCCCAGT
  TCACCCAATCGTGCAGCAGTTACACGGGAACAACATCCACTTCACCGATGGCTACGAGATCAAGGAGGAC
  ATCGGGGTGGGCTCCTACTCAGTGTGCAAGCGATGTGTGCATAAAGCCACAGACACCGAGTATGCCGTGA
  AGATCATTGATAAGAGCAAGAGAGACCCCTCGGAAGAGATTGAGATCCTCCTGCGGTACGGCCAGCACCC
  GAACATCATCACCCTCAAGGATGTCTATGATGATGGCAAGTTTGTGTACCTGGTAATGGAGCTGATGCGT
  GGTGGGGAGCTCCTGGACCGCATCCTCCGGCAGAGATACTTCTCGGAGCGCGAAGCCAGTGACGTCCTGT
  GCACCATCACCAAGACCATGGACTACCTCCATTCCCAGGGGGTTGTTCATCGAGACCTGAAGCCGAGTAA
  CATCCTGTACAGGGATGAGTCGGGGAGCCCAGAATCCATCCGAGTCTGCGACTTCGGCTTTGCCAAGCAG
  CTGCGCGCGGGGAACGGGCTGCTCATGACACCCTGCTACACGGCCAATTTCGTGGCCCCGGAGGTCCTGA
  AGCGTCAAGGCTATGATGCGGCGTGTGACATCTGGAGTTTGGGGATCCTGTTGTACACCATGCTGGCAGG
  ATTTACCCCTTTTGCAAATGGGCCAGACGATACCCCTGAGGAGATTCTGGCGCGGATCGGCAGTGGGAAG
  TATGCCCTTTCTGGGGGAAACTGGGACTCGATATCTGACGCAGCTAAAGACGTCGTGTCCAAGATGCTCC
  ACGTGGACCCTCATCAGCGCCTGACGGCGATGCAAGTGCTCAAACACCCGTGGGTGGTCAACAGAGAGTA
  CCTGTCCCCAAACCAGCTCAGCCGACAGGACGTGCACCTGGTGAAGGGCGCGATGGCCGCCACCTACTTT
  GCTCTAAACAGAACACCTCAGGCCCCGCGGCTGGAGCCCGTGCTGTCATCCAACCTGGCTCAGCGCAGAG
  GCATGAAGAGACTCACGTCCACGCGGCTGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_021135 unedited
  CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGGCGCGGCCT
  GCCCTTTGTGACCGCAGCTCGCGCCCCACGCCCCGCGCCCATGGCCGCCGTGCCGGGCTGGGCGCGGGGC
  GATGGACCTGAGCATGAAGAAGTTCGCCGTGCGCAGGTTCTTCTCTGTGTACCTGCGCAGGAAGTCGCGC
  TCCAAGAGCTCCAGCCTGAGCCGGCTCGAGGAAGAAGGTGTCGTGAAGGAGATAGACATCAGCCATCATG
  TGAAGGAGGGCTTTGAGAAGGCAGATCCTTCCCAGTTTGAGCTGCTGAAAGTTTTAGACAAGGATCCTAT
  GGAAAGGTGTTCCTGGTGAGGAAGTGAAGGGTCCGACGCTGGGCAGCTCTACGCCATGATGGTCCTTAGA
  AGCCACCCTAAAGTTCGGGACCGAGTGAGATCGAGATGAGAAGAGACATCTGCAGAAGTGATCACCCTCA
  TTGTGAGCTCATTATGCTTTCAGACGAGAAGCTCTTACTGATCTGACTCTGGCGGGGAGGGAACCTTTAC
  CGCTCTTCAGAAGGTCTATGTTCACGAGAGAATGTGTCAAGTTAACTGGCTTGACTGCTGCCTTAGACAC
  ATCTCCAGCCTGGGATTCATCTACACAGATTCGAGACCTTGGAAATCCTCTCTGTGTAGAAAGGTACATA
  AGATATACAGATTCGGCCTAGTAAAGAGGGCATTGGCCGACAAGACGCTATCTCTTCGGACATTCAGATA
  CATTGGTCCAGTGTGAACGAGAACCCGAATGCCCACTGGTCTCTCCGCGTTCTATGTTGAAGATGTCTAC
  AGTTCTC
  
  Kinase domain sequence

  >SC323643 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GAMAGGAGGMTCGGGGTGGGCTCCTACTCAGTGTGCAAGCGATGTGTGCATAAAGCCACAGACACCGAGT
  ATGCCGTGATGATCATTGATAAGAGCAAGAGAGACCCCTCGGAAGAGATTGAGATCCTCCTGCGGTACGG
  CCAGCACCCGAACATCATCACCCTCAAGGATGTCTATGATGATGGCAAGTTTGTGTACCTGGTAATGGAG
  CTGATGCGTGGTGGGGAGCTCCTGGACCGCATCCTCCGGCAGAGA
  
• Restriction Sites: Please inquire
• ACCN: NM_021135
• Insert Size: 4310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021135.4, NP_066958.2
• RefSeq Size: 5817 bp
• RefSeq ORF: 2202 bp
• Locus ID: 6196
• Cytogenetics: 6q27
• Domains: pkinase, S_TK_X, TyrKc, PDZ, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: 'This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternative splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.