NEK7 (NM_133494) Human Untagged Clone

CAT#: SC323481

NEK7 (untagged)-Kinase deficient mutant (K63M) of Human NIMA (never in mitosis gene a)-related kinase 7 (NEK7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEK7
• Synonyms: NIMA (never in mitosis gene a)-related kinase 7; NIMA-related kinase 7; OTTHUMP00000033680
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323481 sequence for NM_133494 edited (data generated by NextGen Sequencing)
  ATGGATGAGCAATCACAAGGAATGCAAGGGCCACCTGTTCCTCAGTTCCAACCACAGAAG
  GCCTTACGACCGGATATGGGCTATAATACATTAGCCAACTTTCGAATAGAAAAGAAAATT
  GGTCGCGGACAATTTAGTGAAGTTTATAGAGCAGCCTGTCTCTTGGATGGAGTACCAGTA
  GCTTTAATGAAAGTGCAGATATTTGATTTAATGGATGCCAAAGCACGTGCTGATTGCATC
  AAAGAAATAGATCTTCTTAAGCAACTCAACCATCCAAATGTAATAAAATATTATGCATCA
  TTCATTGAAGATAATGAACTAAACATAGTTTTGGAACTAGCAGATGCTGGCGACCTATCC
  AGAATGATCAAGCATTTTAAGAAGCAAAAGAGGCTAATTCCTGAAAGAACTGTTTGGAAG
  TATTTTGTTCAGCTTTGCAGTGCATTGGAACACATGCATTCTCGAAGAGTCATGCATAGA
  GATATAAAACCAGCTAATGTGTTCATTACAGCCACTGGGGTGGTAAAACTTGGAGATCTT
  GGGCTTGGCCGGTTTTTCAGCTCAAAAACCACAGCTGCACATTCTTTAGTTGGTACGCCT
  TATTACATGTCTCCAGAGAGAATACATGAAAATGGATACAACTTCAAATCTGACATCTGG
  TCTCTTGGCTGTCTACTATATGAGATGGCTGCATTACAAAGTCCTTTCTATGGTGACAAA
  ATGAATTTATACTCACTGTGTAAGAAGATAGAACAGTGTGACTACCCACCTCTTCCTTCA
  GATCACTATTCAGAAGAACTCCGACAGTTAGTTAATATGTGCATCAACCCAGATCCAGAG
  AAGCGACCAGACGTCACCTATGTTTATGACGTAGCAAAGAGGATGCATGCATGCACTGCA
  AGCAGCTAA
  
  Clone variation with respect to NM_133494.2
  188 a=>t;189 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_133494 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAAACTCCCAACTTCC
  TGAGTTCTAAAGTTCCTGTTGCTTCAGACAATGGATGAGCAATCACAAGGAATGCAAGGGCCACCTGTTC
  CTCAGTTCCAACCACAGAAGGCCTTACGACCGGATATGGGCTATAATACATTAGCCAACTTTCGAATAGA
  AAAGAAAATTGGTCGCGGACAATTTAGTGAAGTTTATAGAGCAGCCTGTCTCTTGGATGGAGTACCAGTA
  GCTTTAATGAAAGTGCAGATATTTGATTTATGGGATGCAAAGCACGTGCTGATTGCATCAAAAGAAATAG
  ATTCTTCTTAAGCACTCACCCATCCAAATGTATTAAAAAATAATGCATCATTTCATTGAAGAATAATGAC
  TAAACCTAGTTTTGAACCTAGCCGAATGCCTGGCCGACTATCAGAAGGATTCAAGCCATTTTAAGAAGCA
  AAGAAGGCAATTTCTGAAAGAACTGTTGGAAGTATTTGTTAAGCTTGCATGCATTGGAAACAATGCTTTC
  GAAAGCTAGCTAGAATAACCCAGTTAGGGGTCTTACGCACGGGGGGTAAATTGAATTGGCTGCCGGTTTA
  CTCAAACCACGTCACTCTTATGGACCTATACATGTCCGAGAGAACTGAGATCCTCACTGAACTGGCCTGC
  GTCATAGAGGCGCTACAGCTCTTGGCAATGATATCCTGTGAAGAACGTAAACACTCCTGTACATCGAATC
  GCG
  
  Kinase domain sequence

  >SC323481 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYAAKMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAAACTCCCAACTTCCTGAGTT
  CTAAAGTTCCTGTTGCTTCAGACAATGGATGAGCAATCACAAGGAATGCAAGGGCCACCTGTTCCTCAGT
  TCCAACCACAGAAGGCCTTACGACCGGATATGGGCTATAATACAT
  
• Restriction Sites: Please inquire
• ACCN: NM_133494
• ORF Size: 909 bp
• Insert Size: 4500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_133494.1, NP_598001.1
• RefSeq Size: 1072
• RefSeq ORF: 909
• Locus ID: 140609
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis. [supplied by OMIM, Jul 2002]