RSK4 (RPS6KA6) (NM_014496) Human Untagged Clone

CAT#: SC323492

RPS6KA6 (untagged)-Kinase deficient mutant (K105M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS6KA6
• Synonyms: p90RSK6; PP90RSK4; RSK-4; RSK4; S6K-alpha-6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014496 edited
  ATGCTACCATTCGCTCCTCAGGACGAGCCCTGGGACCGAGAAATGGAAGTGTTCAGCGGC
  GGCGGCGCGAGCAGCGGCGAGGTAAATGGTCTTAAAATGGTTGATGAGCCAATGGAAGAG
  GGAGAAGCAGATTCTTGTCATGATGAAGGAGTTGTTAAAGAAATCCCTATTACTCATCAT
  GTTAAGGAAGGCTATGAGAAAGCAGATCCTGCACAGTTTGAGTTGCTCAAGGTTCTTGGT
  CAGGGGTCATTTGGAAAGGTTTTTCTTGTTAGAAAGAAGACCGGTCCTGATGCTGGGCAG
  CTCTATGCAATGATGGTGTTAAAAAAAGCCTCTTTAAAAGTTCGAGACAGAGTTCGGACA
  AAGATGGAGAGGGATATACTGGTGGAAGTAAATCATCCATTTATTGTCAAATTGCACTAT
  GCCTTTCAGACTGAAGGGAAACTGTACTTAATACTGGATTTTCTCAGGGGAGGAGATGTT
  TTCACAAGATTATCCAAAGAGGTTCTGTTTACAGAGGAAGATGTGAAATTCTACCTCGCA
  GAACTGGCCCTTGCTTTGGATCATCTGCACCAATTAGGAATTGTTTATAGAGACCTGAAG
  CCAGAAAACATTTTGCTTGATGAAATAGGACATATCAAATTAACAGATTTTGGACTCAGC
  AAGGAGTCAGTAGATCAAGAAAAGAAGGCTTACTCATTTTGTGGTACAGTAGAGTATATG
  GCTCCTGAAGTAGTAAATAGGAGAGGCCATTCCCAGAGTGCTGATTGGTGGTCATATGGT
  GTTCTTATGTTTGAAATGCTTACTGGTACTCTGCCATTTCAAGGTAAAGACAGAAATGAG
  ACCATGAATATGATATTAAAAGCAAAACTTGGAATGCCTCAATTTCTTAGTGCTGAAGCA
  CAAAGTCTTCTAAGGATGTTATTCAAAAGGAATCCAGCAAATAGATTGGGATCAGAAGGA
  GTTGAAGAAATCAAAAGACATCTGTTTTTTGCAAATATTGACTGGGATAAATTATATAAA
  AGAGAAGTTCAACCTCCTTTCAAACCTGCTTCTGGAAAACCAGATGATACTTTTTGTTTT
  GATCCTGAATTTACTGCAAAAACACCTAAAGATTCTCCCGGTTTGCCAGCCAGTGCAAAT
  GCTCATCAGCTCTTCAAAGGATTCAGCTTTGTTGCAACTTCTATTGCAGAAGAATATAAA
  ATCACTCCTATCACAAGTGCAAATGTATTACCAATTGTTCAGATAAATGGAAATGCTGCA
  CAATTTGGTGAAGTATATGAATTGAAGGAGGATATTGGTGTTGGCTCCTACTCTGTTTGC
  AAGCGATGCATACATGCAACTACCAACATGGAATTTGCAGTGAAGATCATTGACAAAAGT
  AAGCGAGACCCTTCAGAAGAGATTGAAATATTGATGCGCTATGGACAACATCCCAACATT
  ATTACTTTGAAGGATGTCTTTGATGATGGTAGATATGTTTACCTTGTTACGGATTTAATG
  AAAGGAGGAGAGTTACTTGACCGTATTCTCAAACAAAAATGTTTCTCGGAACGGGAGGCT
  AGTGATATACTATATGTAATAAGTAAGACAGTTGACTATCTTCATTGTCAAGGAGTTGTT
  CATCGTGATCTTAAACCTAGTAATATTTTATACATGGATGAATCAGCCAGTGCAGATTCA
  ATCAGGATATGTGATTTTGGGTTTGCAAAACAACTTCGAGGAGAAAATGGACTTCTCTTA
  ACTCCATGCTACACTGCAAACTTTGTTGCACCTGAGGTTCTTATGCAACAGGGATATGAT
  GCTGCTTGTGATATCTGGAGTTTAGGAGTCCTTTTTTACACAATGTTGGCTGGCTACACT
  CCATTTGCTAATGGCCCCAATGATACTCCTGAAGAGATACTGCTGCGTATAGGCAATGGA
  AAATTCTCTTTGAGTGGTGGAAACTGGGACAATATTTCAGACGGAGCAAAGGATTTGCTT
  TCCCATATGCTTCATATGGACCCACATCAGCGGTATACTGCTGAACAAATATTAAAGCAC
  TCATGGATAACTCACAGAGACCAGTTGCCAAATGATCAGCCAAAGAGAAATGATGTGTCA
  CATGTTGTTAAGGGAGCAATGGTTGCAACATACTCTGCCCTGACTCACAAGACCTTTCAA
  CCAGTCCTAGAGCCTGTAGCTGCTTCAAGCTTAGCCCAGCGACGGAGCATGAAAAAGCGA
  ACATCAACTGGCCTGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014496 unedited
  ACTGCATCGAAACCGCAACGGGCGGAGGCGCGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG
  AACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTCCGTGGAGT
  GGGCAGGTTTACCGAGGCGCTAGCAGGGGCTTTTACTACTCTTCAGTCGCCTCCCCAGCCCAGCTCCCAG
  CCTCTGAGAGACCCGGCGGCGGCGGCGGCGGGCCAGTTCAATAGACAGGATGCGCGGCCGCGCCAGCGGT
  AGGCGGCAGCTCCTGTTTGAGTGCTCCTGAAGGGGAGATGCTACCATTCGCTCCTCAGGGACGAGCCCTG
  GGACCGAGAAAATGGAAGTGTTCAGCGGCGGGCGGCGGCGGAGCAAGCGGCGGAAGGTAAATGTGTGTCT
  AAAAAATGTGTGAGATGGAGCCCATGTGGAAAAGGGAAAACCAGAATCCCTGTGTCATGGATGAAAGAGA
  TTGGGTTAAAGAAAATCCCTAATTACTATTCATGTGTTAAGGGAAGGGCTAGGAAAAGCCCGATTCCCGG
  CACATTTTGATTTTGCTAAGGGTTTTTGGTTCGGGGGCACTTTTGAAAGGTTTTTTTTTTGTTAAAAAAA
  AAACCGGCCCCGGACCCGGGGCCCCCTTTTGCCTGGGGGGGGTTTAAAAAAAGCCCTTTTTAAATTTTCG
  AACAAAATTCGCCACAGTTGGGAGGGGGATTCCGGGGGGGGATTAAAATTCTCCTTTTTTTTCCAATTGG
  CCCTTCCCCTCTCACAGAGAGGGAGACAGTGCTTTTACCGGGGTTTTTCCAGGGGGGGGGAAATTTTTTC
  CACATTTTTCCAGAGGGTGTTGTTTTATAGAGGAGAGATATGTGATTTTTCTCCGAACAGGGACCTTGCT
  TTGTTACTCCCCTCAACTCACGACTATTCTCTAGTGACCACGCAGCAGACGACGATTGTTGTGTGTGAAT
  GAGCCCTTACGTCTTCTCCTTTGTGCTTCTACGAG
  
  Kinase domain sequence

  >SC323492 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AKWYYKKMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCG
  TCAGAATTTTGTAATACGACTCACTATAGGGSGGCCGCGAATTCGGCACGAGGGCTCCGTGGAGTGGGCA
  GGTTTACCGAGGCGCTAGCAGGGGCTTTTACTACTCTTCAGTCGCCTCCCCAGCCCAGCTCCCAGCCTCT
  GAGAGACCCGGCGGCGGCGGCGGCGGGCCAGTTCAATAGACAGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_014496
• ORF Size: 2238 bp
• Insert Size: 5300
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_014496.1, NP_055311.1
• RefSeq Size: 2640
• RefSeq ORF: 2238
• Locus ID: 27330
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: This gene encodes a member of ribosomal S6 kinase family, serine-threonine protein kinases which are regulated by growth factors. The encoded protein may be distinct from other members of this family, however, as studies suggest it is not growth factor dependent and may not participate in the same signaling pathways. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (2) is the same length as isoform 1, but has a distinct N-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.