RSK4 (RPS6KA6) (NM_014496) Human Untagged Clone

CAT#: SC323631

RPS6KA6 (untagged)-Kinase deficient mutant (K455M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS6KA6
• Synonyms: p90RSK6; PP90RSK4; RSK-4; RSK4; S6K-alpha-6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014496, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTACCATTCGCTCCTCAGGACGAGCCCTGGGACCGAGAAATGGAAGTGTTCAGCGGCGGCGGCGCGA
  GCAGCGGCGAGGTAAATGGTCTTAAAATGGTTGATGAGCCAATGGAAGAGGGAGAAGCAGATTCTTGTCA
  TGATGAAGGAGTTGTTAAAGAAATCCCTATTACTCATCATGTTAAGGAAGGCTATGAGAAAGCAGATCCT
  GCACAGTTTGAGTTGCTCAAGGTTCTTGGTCAGGGGTCATTTGGAAAGGTTTTTCTTGTTAGAAAGAAGA
  CCGGTCCTGATGCTGGGCAGCTCTATGCAATGAAGGTGTTAAAAAAAGCCTCTTTAAAAGTTCGAGACAG
  AGTTCGGACAAAGATGGAGAGGGATATACTGGTGGAAGTAAATCATCCATTTATTGTCAAATTGCACTAT
  GCCTTTCAGACTGAAGGGAAACTGTACTTAATACTGGATTTTCTCAGGGGAGGAGATGTTTTCACAAGAT
  TATCCAAAGAGGTTCTGTTTACAGAGGAAGATGTGAAATTCTACCTCGCAGAACTGGCCCTTGCTTTGGA
  TCATCTGCACCAATTAGGAATTGTTTATAGAGACCTGAAGCCAGAAAACATTTTGCTTGATGAAATAGGA
  CATATCAAATTAACAGATTTTGGACTCAGCAAGGAGTCAGTAGATCAAGAAAAGAAGGCTTACTCATTTT
  GTGGTACAGTAGAGTATATGGCTCCTGAAGTAGTAAATAGGAGAGGCCATTCCCAGAGTGCTGATTGGTG
  GTCATATGGTGTTCTTATGTTTGAAATGCTTACTGGTACTCTGCCATTTCAAGGTAAAGACAGAAATGAG
  ACCATGAATATGATATTAAAAGCAAAACTTGGAATGCCTCAATTTCTTAGTGCTGAAGCACAAAGTCTTC
  TAAGGATGTTATTCAAAAGGAATCCAGCAAATAGATTGGGATCAGAAGGAGTTGAAGAAATCAAAAGACA
  TCTGTTTTTTGCAAATATTGACTGGGATAAATTATATAAAAGAGAAGTTCAACCTCCTTTCAAACCTGCT
  TCTGGAAAACCAGATGATACTTTTTGTTTTGATCCTGAATTTACTGCAAAAACACCTAAAGATTCTCCCG
  GTTTGCCAGCCAGTGCAAATGCTCATCAGCTCTTCAAAGGATTCAGCTTTGTTGCAACTTCTATTGCAGA
  AGAATATAAAATCACTCCTATCACAAGTGCAAATGTATTACCAATTGTTCAGATAAATGGAAATGCTGCA
  CAATTTGGTGAAGTATATGAATTGAAGGAGGATATTGGTGTTGGCTCCTACTCTGTTTGCAAGCGATGCA
  TACATGCAACTACCAACATGGAATTTGCAGTGAAGATCATTGACAAAAGTAAGCGAGACCCTTCAGAAGA
  GATTGAAATATTGATGCGCTATGGACAACATCCCAACATTATTACTTTGAAGGATGTCTTTGATGATGGT
  AGATATGTTTACCTTGTTACGGATTTAATGAAAGGAGGAGAGTTACTTGACCGTATTCTCAAACAAAAAT
  GTTTCTCGGAACGGGAGGCTAGTGATATACTATATGTAATAAGTAAGACAGTTGACTATCTTCATTGTCA
  AGGAGTTGTTCATCGTGATCTTAAACCTAGTAATATTTTATACATGGATGAATCAGCCAGTGCAGATTCA
  ATCAGGATATGTGATTTTGGGTTTGCAAAACAACTTCGAGGAGAAAATGGACTTCTCTTAACTCCATGCT
  ACACTGCAAACTTTGTTGCACCTGAGGTTCTTATGCAACAGGGATATGATGCTGCTTGTGATATCTGGAG
  TTTAGGAGTCCTTTTTTACACAATGTTGGCTGGCTACACTCCATTTGCTAATGGCCCCAATGATACTCCT
  GAAGAGATACTGCTGCGTATAGGCAATGGAAAATTCTCTTTGAGTGGTGGAAACTGGGACAATATTTCAG
  ACGGAGCAAAGGATTTGCTTTCCCATATGCTTCATATGGACCCACATCAGCGGTATACTGCTGAACAAAT
  ATTAAAGCACTCATGGATAACTCACAGAGACCAGTTGCCAAATGATCAGCCAAAGAGAAATGATGTGTCA
  CATGTTGTTAAGGGAGCAATGGTTGCAACATACTCTGCCCTGACTCACAAGACCTTTCAACCAGTCCTAG
  AGCCTGTAGCTGCTTCAAGCTTAGCCCAGCGACGGAGCATGAAAAAGCGAACATCAACTGGCCTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014496 unedited
  ACGTACGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTCCGTGGAGTGG
  GCAGGTTTACCGAGGCGCTAGCAGGGGCTTTTACTACTCTTCAGTCGCCTCCCCAGCCCAGCTCCCAGCC
  TCTGAGAGACCCGGCGGCGGCGGCGGCGGGCCAGTTCAATAGACAGGATGCGCGGCCGCGCCAGCGGTAG
  GCGGCAGCTCCTGTTTGAGTGCTCCTGAAGGGGAGATGCTACCATTTCGCTCCTCAGGACGAGCCCCTGG
  GGACCGAGAAAATGGAGGTGGTTCAGCGGGCGGGCGGCGCCGAGCAAGCGGGCAAGGTAAATGTTCTAAA
  AATGGGTTGAATGGAGCCCATGGGAAAAAGGGAAAAGCCGGATTCTTGGTAATGAGAAGGGAGTTGTTAA
  GGAATCCCCAATTACCATATCAGTGTAAAGGAAGGGTAATGGAAAAGCGGTTTCCTGCCCGGTTTGATTT
  GCTCAGGGTTCTGGCACGGGGCAATTTGAAAGGGTTTTTTCTTGTTAAAGAAAACCGGGCCGAGTGCTGG
  GGATCTATGCCCTGGGGGGGTTTTAAAAAACCCTCTAAAATGTGCGACACAGATTTGAGACAGATGTGGA
  GAGGATATCGTGGGAGAATATATCCCCATTATTGCAATTGCCTTCCCTTTCAACTGAGGGAACTGCCTTA
  CCGGGTTTTCCAGGAGAGAATTTTTACACGAATTCCAAAGGTTCGTTCCAGAGATGGAATTTCCTCGAAA
  CTGCCTGTTGATCTCTGCCCATAGATGTTATGACTGAGCCGACACTCGTCTGATGGACTTCATACATGAC
  CACAAGAGTGTATCATAAAG
  
  Kinase domain sequence

  >SC323631 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AGAAGAGWTTGGTGTTGGCTCCTACTCTGTTTGCAAGCGATGCATACATGCAACTACCAACATGGAATTT
  GCAGTGATGATCATTGACAAAAGTAAGCGAGACCCTTCAGAAGAGATTGAAATATTGATGCGCTATGGAC
  AACATCCCAACATTATTACTTTGAAGGATGTCTTTGATGATGGTAGATATGTTTACCTTGTTACGGATTT
  AATGAAAGGAGGAGAGTTACTTGACCGTATTCTCAAACAAAAATG
  
• Restriction Sites: Please inquire
• ACCN: NM_014496
• ORF Size: 2238 bp
• Insert Size: 5000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_014496.1, NP_055311.1
• RefSeq Size: 2640
• RefSeq ORF: 2238
• Locus ID: 27330
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: This gene encodes a member of ribosomal S6 kinase family, serine-threonine protein kinases which are regulated by growth factors. The encoded protein may be distinct from other members of this family, however, as studies suggest it is not growth factor dependent and may not participate in the same signaling pathways. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (2) is the same length as isoform 1, but has a distinct N-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.