STK38 (NM_007271) Human Untagged Clone

CAT#: SC323502

STK38 (untagged)-Kinase deficient mutant (K118M) of Human serine/threonine kinase 38 (STK38)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK38
• Synonyms: NDR; NDR1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323502 sequence for NM_007271 edited (data generated by NextGen Sequencing)
  ATGGCAATGACAGGCTCAACACCTTGCTCATCCATGAGTAACCACACAAAGGAAAGGGTG
  ACAATGACCAAAGTGACACTGGAGAATTTTTATAGCAACCTTATCGCTCAACATGAAGAA
  CGAGAAATGAGACAAAAGAAGTTAGAAAAGGTGATGGAAGAAGAAGGCCTAAAAGATGAG
  GAGAAACGACTCCGGAGATCAGCACATGCTCGGAAGGAAACAGAGTTTCTTCGTTTGAAG
  AGAACAAGACTTGGATTGGAAGATTTTGAGTCCTTAAAAGTAATAGGCAGAGGAGCATTT
  GGTGAGGTACGGCTTGTTCAGAAGAAAGATACGGGACATGTGTATGCAATGATGATACTC
  CGTAAAGCAGATATGCTTGAAAAAGAGCAGGTTGGCCACATTCGTGCGGAGCGTGACATT
  CTAGTGGAGGCAGACAGTTTGTGGGTTGTGAAAATGTTCTATAGTTTTCAGGATAAGCTA
  AACCTCTACCTAATCATGGAGTTCCTGCCTGGAGGGGACATGATGACCTTGTTGATGAAA
  AAAGACACTCTGACAGAAGAGGAGACTCAGTTTTATATAGCAGAAACAGTATTAGCCATA
  GACTCTATTCACCAACTTGGATTCATCCACAGAGACATCAAACCAGACAACCTTCTTTTG
  GACAGCAAGGGCCATGTGAAACTTTCTGACTTTGGTCTTTGCACAGGACTGAAAAAAGCA
  CATAGGACAGAATTTTATAGGAATCTGAACCACAGCCTCCCCAGTGATTTCACTTTCCAG
  AACATGAATTCCAAAAGGAAAGCAGAAACCTGGAAAAGAAATAGACGTCAGCTAGCCTTC
  TCCACAGTAGGCACTCCTGACTACATTGCTCCTGAGGTGTTCATGCAGACCGGGTACAAC
  AAGCTCTGTGATTGGTGGTCGCTTGGGGTGATCATGTATGAGATGCTCATCGGCTACCCA
  CCTTTCTGTTCTGAGACCCCTCAAGAGACATATAAGAAGGTGATGAACTGGAAAGAAACT
  TTGACTTTTCCTCCAGAAGTTCCCATCTCTGAGAAAGCCAAGGATCTAATTTTGAGGTTC
  TGCTGTGAATGGGAACATAGAATTGGAGCTCCTGGAGTTGAGGAAATAAAAAGTAACTCT
  TTTTTTGAAGGCGTTGACTGGGAACATATCAGAGAGAGACCTGCTGCAATATCTATTGAA
  ATCAAAAGCATTGATGATACCTCAAACTTCGATGAGTTTCCAGAATCTGATATTCTTAAG
  CCAACAGTGGCCACAAGTAATCATCCTGAGACTGACTACAAGAACAAAGACTGGGTCTTC
  ATCAATTACACGTACAAGCGCTTTGAGGGCCTGACTGCAAGGGGGGCAATACCTTCCTAC
  ATGAAAGCAGCAAAATAG
  
  Clone variation with respect to NM_007271.2
  353 a=>t;354 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_007271 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGGCTCTGCCAT
  GGCAATGACAGGCTCAACACCTTGCTCATCCATGAGTAACCACACAAAGGAAAGGGTGACAATGACCAAA
  GTGACACTGGAGAATTTTTATAGCAACCTTATCGCTCAACATGAAGAACGAGAAATGAGACAAAAGAAGT
  TAGAAAAGGTGATGGAAGAAGAAGGCCTAAAAGATGAGGAGAAACGACTCCGGAGATCAGCACATGCTCG
  GAAGGAAAACAGAGTTTCTTCGTTTGAAGAAGAACAAGACTTTGGATGGGAAGATTTTGAGTCCTTAAAA
  GTAATAGGCAGAGGAGCATTTGGTGAGGTACGGTTGTTCAGAGAAAGATCCGGGACAGGTGTATGCAATG
  AATGATACTCGTAAACAAATTGCTTAAAAGAACAGTTGGCCAATCGGCGGACTGCTTCATGGGAGCAAAG
  TGGGTTGGAAAGTCTATTTCGGAAGCTACCTACAACTGGATTCCGCTGGGAAGGAACTGTGTAAAAACCT
  GCAAGAAACGTTTTAGAACGTTCTATCTTCCATGGATCCGGACTAACAACTTTTGCAAGGCGTGACTGCC
  TGGTTCGAGCTGAAGCCTGCACATTAAGTTGCACGCGCAGATCTCAGGTCCCCAGCACTGGAAAGCGTAG
  CTCTGAGGCCTGGC
  
  Kinase domain sequence

  >SC323502 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TMGMTTGGATTGGAGWTTTGAGTCCTTAAAGTAATAGGCAGAGGAGCATTTGGTGAGGTACGGCTTGTTC
  AGAAGAAAGATACGGGACATGTGTATGCAATGATGATACTCCGTAAAGCAGATATGCTTGAAAAAGAGCA
  GGTTGGCCACATTCGTGCGGAGCGTGACATTCTAGTGGAGGCAGACAGTTTGTGGGTTGTGAAAATGTTC
  TATAGTTTTCAGGATAAGCTAAACCTCTACCTAATCATGGAGTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_007271
• ORF Size: 1398 bp
• Insert Size: 3500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_007271.2, NP_009202.1
• RefSeq Size: 3593
• RefSeq ORF: 1398
• Locus ID: 11329
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the AGC serine/threonine kinase family of proteins. The kinase activity of this protein is regulated by autophosphorylation and phosphorylation by other upstream kinases. This protein has been shown to function in the cell cycle and apoptosis. This protein has also been found to regulate the protein stability and transcriptional activity of the MYC oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.