STK25 (NM_006374) Human Untagged Clone

CAT#: SC323511

STK25 (untagged)-Kinase deficient mutant (K49M) of Human serine/threonine kinase 25 (STE20 homolog, yeast) (STK25)


  "NM_006374" in other vectors (8)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol STK25
Synonyms SOK1; YSK1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323511 sequence for NM_006374 edited (data generated by NextGen Sequencing)
ATGGCTCACCTCCGGGGATTTGCCAACCAGCACTCTCGAGTGGACCCTGAGGAGCTCTTC
ACCAAGCTCGACCGCATTGGCAAGGGCTCGTTTGGGGAGGTCTACAAGGGCATCGATAAC
CACACAAAGGAGGTGGTGGCCATCATGATCATCGACCTGGAGGAGGCCGAGGATGAGATC
GAGGACATCCAGCAGGAGATCACTGTCCTCAGTCAGTGCGACAGCCCCTACATCACCCGC
TACTTTGGCTCCTACCTAAAGAGCACCAAGCTATGGATCATCATGGAGTACCTGGGCGGC
GGCTCAGCACTGGACTTGCTTAAACCAGGTCCCCTGGAGGAGACATACATTGCCACGATC
CTGCGGGAGATTCTGAAGGGCCTGGATTATCTGCACTCCGAACGCAAGATCCACCGAGAC
ATCAAAGCTGCCAACGTGCTACTCTCGGAGCAGGGTGACGTGAAGCTGGCGGACTTTGGG
GTAGCAGGGCAGCTCACAGACACGCAGATTAAGAGGAACACATTCGTGGGCACCCCCTTC
TGGATGGCACCTGAGGTCATCAAGCAGTCGGCCTACGACTTCAAGGCTGACATCTGGTCC
CTGGGGATCACAGCCATCGAGCTGGCCAAGGGGGAGCCTCCAAACTCTGACCTCCACCCC
ATGCGCGTCCTGTTCCTGATTCCCAAGAACAGCCCACCCACACTGGAGGGCCAGCACAGC
AAGCCCTTCAAGGAGTTCGTGGAGGCCTGCCTCAACAAAGACCCCCGATTCCGGCCCACG
GCCAAGGAGCTCCTGAAGCACAAGTTCATCACACGCTACACCAAGAAGACCTCCTTCCTC
ACGGAGCTCATCGACCGCTATAAGCGCTGGAAGTCAGAGGGGCATGGCGAGGAGTCCAGC
TCTGAGGACTCTGACATTGATGGCGAGGCGGAGGACGGGGAGCAGGGCCCCATCTGGACG
TTCCCCCCTACCATCCGGCCGAGTCCACACAGCAAGCTTCACAAGGGGACGGCCCTGCAC
AGTTCACAGAAGCCTGCGGAGCCCGTCAAGAGGCAGCCGAGGTCCCAGTGCCTGTCCACG
CTGGTCCGGCCCGTCTTCGGAGAGCTCAAAGAGAAGCACAAGCAGAGCGGCGGGAGCGTG
GGTGCGCTGGAGGAGCTGGAGAACGCCTTCAGCCTGGCCGAGGAGTCCTGCCCCGGCATC
TCAGACAAGCTGATGGTGCACCTGGTGGAGCGAGTGCAGAGGTTTTCACACAACAGAAAC
CACCTGACATCCACCCGCTGA

Clone variation with respect to NM_006374.3
146 a=>t
>OriGene 5' read for mutant NM_006374 unedited
ACCGCCCGTTGAGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCCATGGCTCA
CCTCCGGGGATTTGCCAACCAGCACTCTCGAGTGGACCCTGAGGAGCTCTTCACCAAGCTCGACCGCATT
GGCAAGGGCTCGTTTGGGGAGGTCTACAAGGGCATCGATAACCACACAAAGGAGGTGGTGGCCATCATGA
TCATCGACCTGCAGGAGGCCGAGGATGAGATCAAGGACATCCAGCAGGAGATCACTGTCCTCAGTCAGTG
CGACAGCCCCTACATCACCCGCTACTTTGGCTCCTACCTAAAGAGCACCAAGCTATGGATCATCATGAAT
TACCTGGGCAGCGGCTCAGTCTGGACTGGCTAAATCCAGGCCCCGGAGGAAACATACATGGCCCATTCCT
GCGGAAATTCTGAAGGCCGGAATACCTGCCCTCCGACCCCAAATCACCGAAAATAAACCTCCCACCTTGC
ATCTCAGACACAGGGTATTTGACCTGCGGACTTTGGGATCCAGGACTTCAGGACCCCATATAAGAAAACA
TTCTGGGACCCTTTGATGGACTGAGGAATAACATCGGCTAGAATTAAGCGAATTGGTCTGGGTACAGCAT
AGCTGCCAAGGGAGCTCAACTGACTCACCAGCGGCTGTCCGATCCAGAACGCCACACTGAGGCACACAGC
CTAGATCTGAGCTGCTAAAACGATCGGCACGAAGCTGACAGTTCACTCAGGACCTTTCAGACTACGGTAG
CTGATCAG
>SC323511 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCCATGGCTCACCTCCGG
GGATTTGCCAACCAGCACTCTCGAGTGGACCCTGAGGAGCTCTTCACCAAGCTCGACCGCATTGGCAAGG
GCTCGTTTGGGGAGGTCTACAAGGGCATCGATAACCACACAAAGG
Restriction Sites Please inquire     
ACCN NM_006374
ORF Size 1281 bp
Insert Size 5130
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Reference Data
RefSeq NM_006374.3, NP_006365.2
RefSeq Size 2198
RefSeq ORF 1281
Locus ID 10494
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 4 and 5 encode the same isoform (1).

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