STK25 (NM_006374) Human Untagged Clone

CAT#: SC323691

STK25 (untagged)-Kinase deficient mutant (K49M) of Human serine/threonine kinase 25 (STE20 homolog, yeast) (STK25)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK25
• Synonyms: SOK1; YSK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006374, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTCACCTCCGGGGATTTGCCAACCAGCACTCTCGAGTGGACCCTGAGGAGCTCTTCACCAAGCTCG
  ACCGCATTGGCAAGGGCTCGTTTGGGGAGGTCTACAAGGGCATCGATAACCACACAAAGGAGGTGGTGGC
  CATCAAGATCATCGACCTGGAGGAGGCCGAGGATGAGATCGAGGACATCCAGCAGGAGATCACTGTCCTC
  AGTCAGTGCGACAGCCCCTACATCACCCGCTACTTTGGCTCCTACCTAAAGAGCACCAAGCTATGGATCA
  TCATGGAGTACCTGGGCGGCGGCTCAGCACTGGACTTGCTTAAACCAGGTCCCCTGGAGGAGACATACAT
  TGCCACGATCCTGCGGGAGATTCTGAAGGGCCTGGATTATCTGCACTCCGAACGCAAGATCCACCGAGAC
  ATCAAAGCTGCCAACGTGCTACTCTCGGAGCAGGGTGACGTGAAGCTGGCGGACTTTGGGGTAGCAGGGC
  AGCTCACAGACACGCAGATTAAGAGGAACACATTCGTGGGCACCCCCTTCTGGATGGCACCTGAGGTCAT
  CAAGCAGTCGGCCTACGACTTCAAGGCTGACATCTGGTCCCTGGGGATCACAGCCATCGAGCTGGCCAAG
  GGGGAGCCTCCAAACTCTGACCTCCACCCCATGCGCGTCCTGTTCCTGATTCCCAAGAACAGCCCACCCA
  CACTGGAGGGCCAGCACAGCAAGCCCTTCAAGGAGTTCGTGGAGGCCTGCCTCAACAAAGACCCCCGATT
  CCGGCCCACGGCCAAGGAGCTCCTGAAGCACAAGTTCATCACACGCTACACCAAGAAGACCTCCTTCCTC
  ACGGAGCTCATCGACCGCTATAAGCGCTGGAAGTCAGAGGGGCATGGCGAGGAGTCCAGCTCTGAGGACT
  CTGACATTGATGGCGAGGCGGAGGACGGGGAGCAGGGCCCCATCTGGACGTTCCCCCCTACCATCCGGCC
  GAGTCCACACAGCAAGCTTCACAAGGGGACGGCCCTGCACAGTTCACAGAAGCCTGCGGAGCCCGTCAAG
  AGGCAGCCGAGGTCCCAGTGCCTGTCCACGCTGGTCCGGCCCGTCTTCGGAGAGCTCAAAGAGAAGCACA
  AGCAGAGCGGCGGGAGCGTGGGTGCGCTGGAGGAGCTGGAGAACGCCTTCAGCCTGGCCGAGGAGTCCTG
  CCCCGGCATCTCAGACAAGCTGATGGTGCACCTGGTGGAGCGAGTGCAGAGGTTTTCACACAACAGAAAC
  CACCTGACATCCACCCGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_006374 unedited
  ACCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGTCGCTGCCGCC
  ACCACCGTTGCTTCGCGGGCTGGGAGGCCCGGGGTCCCCGGGCGAACAGAGGCTGCGGGTGGGAGCCTTC
  GCGGGCGCTGCAGAGCGGGCCGGGGGAGGCCGGGATGGAGCCCCGCGGAGCACTCTCGAGTGGACCCTGA
  GGAGCTCTTCACCAAGCTCGACCGCATTGGCAAGGGCTCGTTTGGGGAGGTCTACAAAGGGCATCGATAA
  CCACACAAAGGAGTGGTGGCCATCATGATCATCGACCCTGAGGAGGCCGAGGATGAGATCGAGGACATCC
  AGCAGGAGATCACTTTCCTCAGTCAGTGCGACAGCCCCTACATCACCCGCTACTTTGGCTCTACCTAAGG
  AGCACAGCTTTGGATCATCATGGAGTACCTGGGCGCGCTCAGCCACTGACTTGCTTAATCAGTTCCCTGA
  AGAAACCTAACTTGCCCGATCCTGCCGGAAGATTCTGCGCCTGATATCTTGAATCTCGACCCAGAATTCC
  CACCGGAACCTCTCAAGCTGCGCCACCTGGCTCATCTCGGACACAGTGTAGACTGTAGACCTGCGGGACA
  CTTTGTGATCACGGCACTCTCAGACACGCGATATAGAGGGAACTCTCTGTGCACACCTCTTCGATAGTGA
  ACTGAGTGACTCTAGCAGTCTGCCTTCACATCAAGCGTCATATCTGTGCTGATATCAGCTCTAGACTGCT
  GCACAGGAAACTCTACCTTGGATTCTCCATCGGCCTCTGTTCGATTCCGAAGGCCCCCACTTGAGGGCCG
  AAGCCCTCTGGAGATTCCTGGAGAGCGTCCTCAT
  
  Kinase domain sequence

  >SC323691 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYTGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG
  AATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGTCGCTGCCGCCACCACCGT
  TGCTTCGCGGGCTGGGAGGCCCGGGGTCCCCGGGCGAACAGAGGCTGCGGGTGGGAGCCTTCGCGGGCGC
  TGCAGAGCGGGCCGGGGGAGGCCGGGATGGAGCCCCGCGGAGCAC
  
• Restriction Sites: Please inquire
• ACCN: NM_006374
• ORF Size: 1281 bp
• Insert Size: 5130
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_006374.3, NP_006365.2
• RefSeq Size: 2198
• RefSeq ORF: 1281
• Locus ID: 10494
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 4 and 5 encode the same isoform (1).