ADCK2 (NM_052853) Human Untagged Clone

CAT#: SC323527

ADCK2 (untagged)-Kinase deficient mutant (K311M) of Human aarF domain containing kinase 2 (ADCK2)


  "NM_052853" in other vectors (7)

Reconstitution Protocol

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    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ADCK2
Synonyms AARF
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323527 sequence for NM_052853 edited (data generated by NextGen Sequencing)
ATGGTGGCGCCCTGGCGCGTCTCCGTCAGGGTTTGCCTGTCGCACCTGAGGTGCTTCGAG
CTCAGACAGGGACTCAGCCTCCTGAGGCCCTCCGAGTGCCCTCGCGATGCCAGGCTCTGC
TGGCTTCTGCTGGGCACTTTGCCCAAGGTCGTCTCCCTGTGCGGGGACGTGGGTGAGGGG
GCCCCTGACGTTCTGAGTCGGCGAAGGGTCCGCTGCAGCGGGGCGGCTGGCGCGGGGCCC
GCGGAGAGCCTCCCCCGAGCGGGACCTCTGGGCGGCGTCTTCCTGCATCTCCGCCTCTGG
CTTCGCGCCGGCGCTCTGTTGGTGAAATTCTTCCCCCTCCTACTCCTCTACCCCCTCACC
TACCTGGCTCCCAGCGTCTCCACCCTCTGGCTCCACCTGCTTCTGAAAGCCACCGAGACC
TCAGGCCCAACCTACATCAAACTGGGCCAGTGGGCCAGCACCCGGCGCGATCTGTTTTCG
GAGGCTTTCTGTGCCCAATTTTCCAAGCTGCATGTCCGAGTGACGCCCCACCCGTGGACT
CACACTGAGCGCTTCCTTCGGCAGGCTTTTGGGGATGACTGGGGGAGCATCCTCTCTTTT
GAGAACCGGGAACCTGTGGGCTCAGGCTGCGTGGCCCAGGTGTACAAAGCATACGCCAAC
ACTGCCTTCCTGGAGACTGACAGCGTCCAGAGACTTGGCAGGGCCTCCTGTCTGCCGCCC
TTCTCACATACTGGGGCAGTCGGTGGGCTGAGAGAGCTCTTTGGATACCTTGGAAATGGC
CGGAAACCTCCAGAAAATCTCGCAGACCAGTCGTTTCTAGAAAGGCTGCTCCTCCCTAAA
GCTGACCTGGTTGGATCAAATGCAGGGGTGTCTCGGGCTCAGGTCCCTGGCCACCAACCT
GAGGCCACCAACCTCATCTCCGTGGCAGTGATGGTGTTGCACCCTGGCCTGCTCGCTCAG
GTGCATATGGACCTGCTGCTGATGAAGATTGGCAGCCGAGTCCTGGGAGTTTTGCCAGGC
ATCAAGTGGCTTAGCTTGCCTGAGATTGTGGAGGAATTTGAGAAGCTGATGGTCCAACAG
ATTGACCTGCGTTACGAAGCTCAGAATCTAGAACACTTCCAGGTCAACTTCCGGAATGTG
AAAGCCGTCAAGTTCCCCACCCCTCTGCGCCCCTTTGTCACCAGAGAAGTCTTGGTGGAA
ACGTATGAAGAGAGTGTGCCTGTGTCCAGTTACCAGCAGGCAGGAATTCCCGTGGACTTG
AAAAGGAAGATTGCACGGCTGGGGATCAACATGCTCCTGAAGATGATATTTGTGGATAAC
TTTGTCCATGCAGACCTTCACCCTGGAAACATCCTGGTTCAGGGTGCCAACGGCCTGTCC
TCGAGTCAGGAGGCGCAGCTGCAGCAGGCGGACATCTGTGACACTCTGGTGGTGGCCGTG
CCATCTTCCCTCTGCCCGCTGCGACTGGTGCTGCTGGATGCTGGCATTGTGGCGGAGCTG
CAGGCCCCTGACCTGAGGAATTTCCGGGCAGTTTTCATGGCTGTGGTGATGGGGCAGGGC
CAGAGAGTGGCTGAGCTGATCCTGCATCATGCCCGGGCCAGCGAGTGCAGGGACGTGGAG
GGGTTCAAAACCGAGATGGCCATGCTGGTGACCCAGGCCAGGAAGAACACCATCACCCTG
GAGAAGCTTCATGTGTCCAGCCTTCTCTCTAGTGTCTTTAAGTTGCTGATGACTCACAAG
GTAAAGCTTGAGAGCAACTTTGCCTCCATTGTGTTTGCCATCATGGTGTTGGAGGGGCTT
GGCCGCTCACTGGACCCCAAACTGGACATCCTGGAGGCAGCGAGGCCCTTCCTCCTCACG
GGCCCAGTGTGCCCCCCGTGA

Clone variation with respect to NM_052853.3
932 a=>t;933 a=>g
>OriGene 5' read for mutant NM_052853 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGCTCTGAAGTGGAGGGCGGGCCGCCTGGGC
CGCGGGCCTCGGGAGGATGGTGGCGCCCTGGCGCGTCTCCGTCAGGGTTTGCCTGTCGCACCTGAGGTGC
TTCGAGCTCAGACAGGGACTCAGCCTCCTGAGGCCCTCCGAGTGCCCTCGCGATGCCAGGCTCTGCTGGC
TTCTGCTGGGCACTTTGCCCAAGGTCGTCTCCCTGTGCGGGGACGTGGGTGAGGGGGCCCCTGACGTTCT
GAGTCGGCGAAGGGTCCGCTGCAGCGGGGCGGCTGGCGCGGGGCCGCGGAGAGCCTCCCCCGAGCGGGAC
CTCTGGGCGGCGTCTTCCTGCATCTCCGCCTCTGCTTCGCGCGCGCTCTGTTGTGAAATCTCCCCTCTAC
TCTCTACCCCCTCACCTACTGCTCAACGTTCTCACCTTCTGCTTCACTGCTTCTGAAAGCACGAAACCTC
AGCCAACTAACTCAACTGGCAATGGACCAGCCACCCGGCGCGATATCGGTTTCCGAGAGCTTCTGGCCAA
TTACAAGACGTCGACAGTGTCCCAATGTAGACGCCACACCTGACACTCCACTTAGAGCGTTCTCTCTGCA
GAGCGCTTTAGGATGAGATCTACGTGGGGAGACTCATCTCTCTTTAGACCGGAACCTGAGCTCTAGCGTC
CTGTGCCAAGTCCAAGCTAGCCAACTGCGTCTCGAATCATGAAGTCTCGACTGTAGAGGCCCATGCTGTA
CGCCTCTCACTACTGGGAACATGCGTGAGACCTGTGACACTGATAGCGCAACTCTAATCGGCAGCGTCTA
GAGGCCTCCCTCACATGAACAGCTATGGATTGA
>SC323527 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GYYCGGGCTAGTCCTGGCMCCACCTGAGGCCACCAACCTCATCTCCGTGGCAGTGATGGTGTTGCACCCT
GGCCTGCTCGCTCAGGTGCATATGGACCTGCTGCTGATGAAGATTGGCAGCCGAGTCCTGGGAGTTTTGC
CAGGCATCAAGTGGCTTAGCTTGCCTGAGATTGTGGAGGAATTTGAGAAGCTGATGGTCCAACAGATTGA
CCTGCGTTACGAAGCTCAGAATCTAGAACACTTCCAGGTCAACTT
Restriction Sites Please inquire     
ACCN NM_052853
ORF Size 1881 bp
Insert Size 2250
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Reference Data
RefSeq NM_052853.3, NP_443085.2
RefSeq Size 2376
RefSeq ORF 1881
Locus ID 90956
Domains ABC1
Protein Families Druggable Genome, Protein Kinase
Gene Summary The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr). [UniProtKB/Swiss-Prot Function]

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