MAP4K1 (NM_007181) Human Untagged Clone

CAT#: SC323528

MAP4K1 (untagged)-Kinase deficient mutant (K46M) of Human mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP4K1
• Synonyms: HPK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007181, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGG
  GTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAGAT
  GGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTGCCGG
  CACGCCAACATCGTGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAATTCT
  GTGGGGCTGGTTCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGATTAGCTATGT
  CTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCACAGAAGAAGATACACAGGGACATCAAGGGA
  GCTAACATCCTCATCAATGATGCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAGATTGGGG
  CTACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTCCGGAAGTGGCAGCTGTGGC
  CCTGAAGGGAGGATACAATGAGCTGTGTGACATCTGGTCCCTGGGCATCACGGCCATCGAACTGGCCGAG
  CTACAGCCACCGCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGTGGCTACCAGC
  CTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTCCACAACTTCATCAAAGTCACTCTGACTAA
  GAGTCCCAAGAAACGACCCAGCGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAGCCTGGGCTGAAT
  CGAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGACCCTCCATTGGGGACATTG
  AGGATGAGGAGCCCGAGCTACCCCCTGCTATCCCTCGGCGGATCAGATCCACCCACCGCTCCAGCTCTCT
  GGGGATCCCAGATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGAGGAATGGAGACCAGA
  CCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCTCAGGAGCAGCAGCCCCAGGAAGCAACTGT
  CAGAGTCGTCTGACGATGACTATGACGACGTGGACATCCCCACCCCTGCAGAGGACACACCTCCTCCACT
  TCCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCATGGGGGATGATGGGCAGCTG
  AGCCCGGGGGTGCTGGTCCGGTGTGCCAGTGGGCCCCCACCAAACAGCCCCCGTCCTGGGCCTCCCCCAT
  CCACCAGCAGCCCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAACCCACCCTCCCGGGAGCTTGA
  CAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGAGAAAGGGATGTGCCCTTCTCGTAAAGTTG
  TTCAATGGCTGCCCCCTCCGGATCCACAGCACGGCCGCCTGGACACATCCCTCCACCAAGGACCAGCACC
  TGCTCCTGGGGGCAGAGGAAGGCATCTTCATCCTGAACCGGAATGACCAGGAGGCCACGCTGGAAATGCT
  CTTTCCTAGCCGGACTACGTGGGTGTACTCCATCAACAACGTTCTCATGTCTCTCTCAGGAAAGACCCCC
  CACCTGTATTCTCATAGCATCCTTGGCCTGCTGGAACGGAAAGAGACCAGAGCAGGAAACCCCATCGCTC
  ACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTTTCCACCAAGATCCAGGACACCAAAGGCTG
  CCGGGCGTGCTGTGTGGCGGAGGGTGCGAGCTCTGGGGGCCCGTTCCTGTGCGGTGCATTGGAGACGTCC
  GTTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTGCTTGTCCGGCAGGTGCTGTTCCCACTGC
  CGACGCCTCTGTCCGTGTTCGCGCTGCTGACCGGGCCAGGCTCTGAGCTGCCCGCTGTGTGCATCGGCGT
  GAGCCCCGGGCGGCCGGGGAAGTCGGTGCTCTTCCACACGGTGCGCTTTGGCGCGCTCTCTTGCTGGCTG
  GGCGAGATGAGCACCGAGCACAGGGGACCCGTGCAGGTGACCCAGGTAGAGGAAGATATGGTGATGGTGT
  TGATGGATGGCTCTGTGAAGCTGGTGACCCCGGAGGGGTCCCCAGTCCGGGGACTTCGCACACCTGAGAT
  CCCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTTGGAGGTCAGCTTCAGGCCTTCTGGAAGCATGGA
  GTGCAGGTGTGGGCTCTAGGCTCGGATCAGCTGCTACAGGAGCTGAGAGACCCTACCCTCACTTTCCGTC
  TGCTTGGCTCCCCCAGGCTGGAGTGCAGTGGCACGATCTCGCCTCACTGCAACCTCCTCCTCCCAGGTTC
  AAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCCTGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_007181 unedited
  ACGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCAGGCCCCCAGGG
  ATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGG
  GTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGATGAT
  GGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTTGCCG
  GCACGCCAACATCGTGGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAAACTCTGGATCTGCATGGAAT
  TCTGTGGGGCTGGTTCTCTCCAGACCATCTACCAGTTGACAGGCTCCCTGGTCGAACCTCCGATAACCTA
  TGTCTGCCGGGAGTTGCTCAGGGACTGCCCTATTTGCCCTACCGAAGAAGAATACAAGGGCCCTCAAGGG
  ACTTACCTTCCTAATCATTGATGCTGGGAAGGTCAGATTGGCTGACTTGGACTCTCGCCCAAATTGGCTC
  ACCCTGCCGAACCCCTCTTTTCTTGGGCACCCTATCTGAAGGGCTCGAATGGGCCCTTGGCCCGAGAGGA
  GAGAACAATAGCCTGTGTGACTGTGTCCGGGGTACAGGACATAAAGGGCACGACTACCCACGCTTGTGTG
  TGCCCCTAGATCTCTCTCTTACACAATGTGTCAGCGTCGCACATGAAAAGACAAAGTGTCGCTTCCATCT
  CAGTCTCGCGCAAGGTGCGGAGGACTCGCGAAGCCTAACTGTCACTCGTTAAGCGACTGATCTATGAA
  
  Kinase domain sequence

  >SC323528 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AKKATGMGCAATGGGCGGTAGSGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCAGGCCCCCAGGGATGG
  ACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGGGTGG
  CGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_007181
• ORF Size: 2502 bp
• Insert Size: 2900
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_007181.3, NP_009112.1
• RefSeq Size: 2732
• RefSeq ORF: 2502
• Locus ID: 11184
• Domains: pkinase, CNH, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway