AAK1 (BC002695) Human Untagged Clone

CAT#: SC323545

AAK1 (untagged)-Kinase deficient mutant (K74M) of Homo sapiens, Similar to KIAA1048 protein, clone MGC:3535 IMAGE:3607681, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AAK1
• Synonyms: adaptor-associated kinase 1; AP2 associated kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC002695, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGAAGTTTTTCGACTCCCGGCGAGAGCAGGGCGGCTCTGGCCTGGGCTCCGGCTCCAGCGGAGGAG
  GGGGCAGCACCTCGGGCCTGGGCAGTGGCTACATCGGAAGAGTCTTCGGCATCGGGCGACAGCAGGTCAC
  AGTGGACGAGGTGTTGGCGGAAGGTGGATTTGCTATTGTATTTCTGGTGAGGACAAGCAATGGGATGAAA
  TGTGCCTTGAAACGCATGTTTGTCAACAATGAGCATGATCTCCAGGTGTGCAAGAGAGAAATCCAGATAA
  TGAGGGATCTTTCAGGGCACAAGAATATTGTGGGTTACATTGATTCTAGTATCAACAACGTGAGTAGCGG
  TGATGTATGGGAAGTGCTCATTCTGATGGACTTTTGTAGAGGTGGCCAGGTGGTAAACCTGATGAACCAG
  CGCCTGCAAACAGGCTTTACAGAGAATGAAGTGCTCCAGATATTTTGTGATACCTGTGAAGCTGTTGCCC
  GCCTGCATCAGTGCAAAACTCCTATTATCCACCGGGACCTGAAGGTTGAAAACATCCTCTTGCATGACCG
  AGGCCACTATGTCCTGTGTGACTTTGGAAGCGCCACCAACAAATTCCAGAATCCACAAACTGAGGGAGTC
  AATGCAGTAGAAGATGAGATTAAGAAATACACAACGCTGTCCTATCGAGCACCAGAAATGGTCAACCTGT
  ACAGTGGCAAAATCATCACTACGAAGGCAGACATTTGGGCTCTTGGATGTTTGTTGTATAAATTATGCTA
  CTTCACTTTGCCATTTGGGGAAAGTCAGGTGGCAATTTGTGATGGAAACTTCACAATTCCTGATAATTCT
  CGATATTCTCAAGACATGCACTGCCTAATTAGGTATATGTTGGAACCAGACCCTGACAAAAGGCCGGATA
  TTTACCAGGTGTCCTACTTCTCATTTAAGCTACTCAAGAAAGAGTGCCCAATTCCAAATGTACAGAACTC
  TCCCATTCCTGCAAAGCTTCCTGAACCAGTGAAAGCCAGTGAGGCAGCTGCAAAAAAGACCCAGCCAAAG
  GCCAGACTGACAGATCCCATTCCCACCACAGAGACTTCAATTGCACCCCGCCAGAGGCCTAAAGCTGGGC
  AGACTCAGCCGAACCCAGGAATCCTTCCCATCCAGCCAGCGCTGACACCCCGGAAGAGGGCCACTGTTCA
  GCCCCCACCTCAGGCTGCAGGATCCAGCAATCAGCCTGGCCTTTTAGCCAGTGTTCCCCAACCAAAACCC
  CAAGCCCCACCCAGCCAGCCTCTGCCGCAAACTCAGGCCAAGCAGCCACAGGCTCCTCCCACTCCACAGC
  AGACGCCTTCTACTCAGGCCCAGGGTCTGCCCGCTCAGGCCCAGGCCACACCCCAGCACCAGCAGCATAC
  AATAAAACTTAGTATGAAACTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant BC002695 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAAACCATCGGTATTT
  TGCTTTGCTGCTCCCTATTCGCAAGATGAAGAAGCTTTTCGACTCCCGGCGAGAGCAGGGCGGCTCTGGC
  CTGGGCTCCGGCTCCAGCGGAGGAGGGGGCAGCACCTCGGGCCTGGGCAGTGGCTACATCGGAAGAGTCT
  TCGGCATCGGGCGACAGCAGGGTCACAGTGGACGAGGGTGTTGGCGGGAAGTGGATTTGCTATTTGTATT
  TTCTGGGTGAGGGACAAGCCAATGGGATGAAAATGTGGCCTTGATAGCCCGCTAGTGTTTGTCCAACAAT
  GGAGCACATGGATTCTCCCAGTGTGTGCAAAGAGAGAGAAATCTCACGATATATGAGAGGATCCCTTTCA
  GGGGCACCAAGAAATTTGGGTGGGTACACATGAGATCTCTAGTAAAAAACACCTTGAATGGCGGTGTATT
  ATAGGGAGAATGTGCTTATTCGTTATGGCCTTTTGTAGGGGGTGGCCAGGTGGTAACCCTGATGAACCCC
  CGCCCCTGGAAAACGCGCTTTCCAAAAAAAGGAAGGGGCCCAAAATTTTTGGAATCCGTGAAAACCTTTT
  CCCCCCCCTCTCAGGCAAATCCTCTTATTATCCCCGACCCGCAGGTGGTAAAAATCCTTCTTGTTGAAAA
  AGGAGCGCCAAAGCCCGGGGGCAACGGAGAACCCCCCCCCAAATTCTCAAAACCCCACCTAGGGAGGCAT
  TGCGGTCTAGAAAGAGAAATAAAGAACAACGCGTGCTCTCTATCGCACACAAAGTGTGCCCTCTGTGTGG
  CGATATTCTCTCAAGCGAGACATTTGCCGCTGAGATGTGTGTTGAATATACCGAGCACCTGCGCAATCGG
  AACCATGGCGCAGTGGTTGGGAGCTACACTCTGCAGATATACATACTGAAGCACGTCGCGCTCTATGATG
  T
  
  Kinase domain sequence

  >SC323545 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CRKCTRAGTGTGGCGGAGGTGGATTTGCTATTGTATTTCTGGTGAGGACAAGCAATGGGATGAAATGTGC
  CTTGATGCGCATGTTTGTCAACAATGAGCATGATCTCCAGGTGTGCAAGAGAGAAATCCAGATAATGAGG
  GATCTTTCAGGGCACAAGAATATTGTGGGTTACATTGATTCTAGTATCAACAACGTGAGTAGCGGTGATG
  TATGGGAAGTGCTCATTCTGATGGACTTTTGTAGAGGTGGCCAGG
  
• Restriction Sites: Please inquire
• ACCN: BC002695
• ORF Size: 1425 bp
• Insert Size: 1650
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: BC002695.2, AAH02695.1
• RefSeq Size: 1513
• RefSeq ORF: 1425
• Locus ID: 22848
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]