CAMK2A (NM_171825) Human Untagged Clone

CAT#: SC323546

CAMK2A (untagged)-Kinase deficient mutant (K42M) of Human calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAMK2A
• Synonyms: CAMKA; CaMKIINalpha; MRD53; MRT63
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323546 sequence for NM_171825 edited (data generated by NextGen Sequencing)
  ATGGCCACCATCACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTGGGC
  AAGGGAGCCTTCTCGGTGGTGCGAAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTATGCT
  GCCAAGATCATCAACACAAAGAAGCTGTCAGCCAGAGACCATCAGAAGCTGGAGCGTGAA
  GCCCGCATCTGCCGCCTGCTGAAGCACCCCAACATCGTCCGACTACATGACAGCATCTCA
  GAGGAGGGACACCACTACCTGATCTTCGACCTGGTCACTGGTGGGGAACTGTTTGAAGAT
  ATCGTGGCCCGGGAGTATTACAGTGAGGCGGATGCCAGTCACTGTATCCAGCAGATCCTG
  GAGGCTGTGCTGCACTGCCACCAGATGGGGGTGGTGCACCGGGACCTGAAGCCTGAGAAT
  CTGTTGCTGGCCTCCAAGCTCAAGGGTGCCGCAGTGAAGCTGGCAGACTTTGGCCTGGCC
  ATAGAGGTGGAGGGGGAGCAGCAGGCATGGTTTGGGTTTGCAGGGACTCCTGGATATCTC
  TCCCCAGAAGTGCTGCGGAAGGACCCGTACGGGAAGCCTGTGGACCTGTGGGCTTGTGGG
  GTCATCCTGTACATCCTGCTGGTTGGGTACCCCCCGTTCTGGGATGAGGACCAGCACCGC
  CTGTACCAGCAGATCAAAGCCGGCGCCTATGATTTCCCATCGCCGGAATGGGACACTGTC
  ACCCCGGAAGCCAAGGATCTGATCAATAAGATGCTGACCATTAACCCATCCAAACGCATC
  ACAGCTGCCGAAGCCCTTAAGCACCCCTGGATCTCGCACCGCTCCACCGTGGCATCCTGC
  ATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAG
  GGAGCCATTCTCACCACGATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGGGGA
  AACAAGAAGAGCGATGGTGTGAAGGAATCCTCAGAGAGCACCAACACCACCATCGAGGAT
  GAAGACACCAAAGTGCGGAAACAGGAAATTATAAAAGTGACAGAGCAGCTGATTGAAGCC
  ATAAGCAATGGAGATTTTGAGTCCTACACGAAGATGTGCGACCCTGGCATGACAGCCTTC
  GAACCTGAGGCCCTGGGGAACCTGGTTGAGGGCCTGGACTTCCATCGATTCTATTTTGAA
  AACCTGTGGTCCCGGAACAGCAAGCCCGTGCACACCACCATCCTGAATCCCCACATCCAC
  CTGATGGGCGACGAGTCAGCCTGCATCGCCTACATCCGCATCACGCAGTACCTGGACGCT
  GGCGGCATCCCACGCACCGCCCAGTCGGAGGAGACCCGTGTCTGGCACCGCCGGGATGGC
  AAATGGCAGATCGTCCACTTCCACAGATCTGGGGCGCCCTCCGTCCTGCCCCACTGA
  
  Clone variation with respect to NM_171825.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_171825 unedited
  CCGCCGTTTCAGCAATGGGCGGTAGGCGTTGTACGGTGGGAGGTTCTATATAAGCAGAGCTCATATTAGG
  TTGACACTATAGAATACAAGCTACTATGTTCTTTTTGCAGCGGCCGCGAATTTCGGCACGAGGCTCGTCA
  GTCAAACCGGTACTCTGTTTGCACTCGGCAGCACGGGCAGGCAAGAGGTCCCTAGGTTCGGGAGCAGAGC
  AGCAGCGCCTCAGTCCTGGTCCCCCAGTCCCAAGCCTCACCTGCCTGCCCAGCGCCAGGATGGCCACCAT
  CACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTGGGCAAGGGAGCCCTTCTCGGGTGG
  TGCGGAAGGTGTGTGGAAGTTGCTTGGCTTGGCCAGAAGTATTGGCTGGCCTTGATTCTTCAACACCAAA
  AAAACCTGTCACCCAGAGAACAATTCGAGAGCCCTGGAAGCGTTGAGCCCCCGCATCCTGCGCGCCTGCT
  GAACACCCCCCACCATCGTTCGACACTACTGAAAACCATCCTCAAAAGAAGGAAACCCCCTTTCCTGATT
  TTTCACCCGGGTCCTGGGGGGGGAAACGGTTTTAAAAAATTTTGGGCCCCGGGGTTTTTAACTGGAGGGG
  GGGAACCCCTTTCCGGGTTCCCCCCAAAATCCGGGGGCGGTGGGTGCTCCCTGCCCCCAAAAGGGGGGGG
  GGCCCCCCGGAGCCGTAAACCCCTGAAAATTTTTTTGGGGCCCCCAACTCCCAGGGGGGCCCCCTGAGAC
  CCGGGAAATTTTTGCCCCCCCCCTAGGGGGGGGGGGGGAACCCCCCAGGTTTTTTTATATTCCTGAAGAA
  CCCCCCCAGGGGACCCCGGGGCCCCGGGGTTTTGGGGCACCCCGCACCCCGCGGGGTGGGGAACCACCCC
  TTTTGGGAGAAGAGAACACACGCCCGTATACACCATATACACCGGCCGCTGTAGATTCTCCTCCGCGAGT
  GAATGTAGTACCCCCCACACGCAGACGTGATTATATAAGTGTATACGA
  
  Kinase domain sequence

  >SC323546 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ASAATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACT
  ATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCTCGTCAGTCAAACCGG
  TTCTCTGTTTGCACTCGGCAGCACGGGCAGGCAAGTGGTCCCTAGGTTCGGGAGCAGAGCAGCAGCGCCT
  CAGTCCTGGTCCCCCAGTCCCAAGCCTCACCTGCCTGCCCAGCGC
  
• Restriction Sites: Please inquire
• ACCN: NM_171825
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_171825.1, NP_741960.1
• RefSeq Size: 4803 bp
• RefSeq ORF: 1437 bp
• Locus ID: 815
• Cytogenetics: 5q32
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Glioma, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Wnt signaling pathway
• Gene Summary: 'The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018]'
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 3. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Variants 2 and 4 both encode the same isoform (2). Isoforms 2 and 5 are the same length but differ in sequence. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.