Snf1lk (SIK1) (NM_173354) Human Untagged Clone

CAT#: SC323562

SIK1 (untagged)-Kinase deficient mutant (K56M) of Human salt-inducible kinase 1 (SIK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIK1
• Synonyms: MSK; SIK; SIK-1; SIK1B; SNF1LK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323562 sequence for NM_173354 edited (data generated by NextGen Sequencing)
  ATGGTTATCATGTCGGAGTTCAGCGCGGACCCCGCGGGCCAGGGTCAGGGCCAGCAGAAG
  CCCCTCCGGGTGGGTTTTTACGACATCGAGCGGACCCTGGGCAAAGGCAACTTCGCGGTG
  GTGAAGCTGGCGCGGCATCGAGTCACCAAAACGCAGGTTGCAATAATGATAATTGATAAA
  ACACGATTAGATTCAAGCAATTTGGAGAAAATCTATCGTGAGGTTCAGCTGATGAAGCTT
  CTGAACCATCCACACATCATAAAGCTTTACCAGGTTATGGAAACAAAGGACATGCTTTAC
  ATCGTCACTGAATTTGCTAAAAATGGAGAAATGTTTGATTATTTGACTTCCAACGGGCAC
  CTGAGTGAGAACGAGGCGCGGAAGAAGTTCTGGCAAATCCTGTCGGCCGTGGAGTACTGT
  CACGACCATCACATCGTCCACCGGGACCTCAAGACCGAGAACCTCCTGCTGGATGGCAAC
  ATGGACATCAAGCTGGCAGATTTTGGATTTGGGAATTTCTACAAGTCAGGAGAGCCTCTG
  TCCACGTGGTGTGGGAGCCCCCCGTATGCCGCCCCGGAAGTCTTTGAGGGGAAGGAGTAT
  GAAGGCCCCCAGCTGGACATCTGGAGCCTGGGCGTGGTGCTGTACGTCCTGGTCTGCGGT
  TCTCTCCCCTTCGATGGGCCTAACCTGCCGACGCTGAGACAGCGGGTGCTGGAGGGCCGC
  TTCCGCATCCCCTTCTTCATGTCTCAAGACTGTGAGAGCCTGATCCGCCGCATGCTGGTG
  GTGGACCCCGCCAGGCGCATCACCATCGCCCAGATCCGGCAGCACCGGTGGATGCGGGCT
  GAGCCCTGCTTGCCGGGACCCGCCTGCCCCGCCTTCTCCGCACACAGCTACACCTCCAAC
  CTGGGCGACTACGATGAGCAGGCGCTGGGTATCATGCAGACCCTGGGCGTGGACCGGCAG
  AGGACGGTGGAGTCACTGCAAAACAGCAGCTATAACCACTTTGCTGCCATTTATTACCTC
  CTCCTTGAGCGGCTCAAGGAGTATCGGAATGCCCAGTGCGCCCGCCCCGGGCCTGCCAGG
  CAGCCGCGGCCTCGGAGCTCGGACCTCAGTGGTTTGGAGGTGCCTCAGGAAGGTCTTTCC
  ACCGACCCTTTCCGACCTGCCTTGCTGTGCCCGCAGCCGCAGACCTTGGTGCAGTCCGTC
  CTCCAGGCCGAGATGGACTGTGAGCTCCAGAGCTCGCTGCAGTGGCCCTTGTTCTTCCCG
  GTGGATGCCAGCTGCAGCGGAGTGTTCCGGCCCCGGCCCGTGTCCCCAAGCAGCCTGCTG
  GACACAGCCATCAGTGAGGAGGCCAGGCAGGGGCCGGGCCTAGAGGAGGAGCAGGACACG
  CAGGAGTCCCTGCCCAGCAGCACGGGCCGGAGGCACACCCTGGCCGAGGTCTCCACCCGC
  CTCTCCCCACTCACCGCGCCATGTATAGTCGTCTCCCCCTCCACCACGGCAAGTCCTGCA
  GAGGGAACCAGCTCTGACAGTTGTCTGACCTTCTCTGCGAGCAAAAGCCCCGCGGGGCTC
  AGTGGCACCCCGGCCACTCAGGGGCTGCTGGGCGCCTGCTCCCCGGTCAGGCTGGCCTCG
  CCCTTCCTGGGGTCGCAGTCCGCCACCCCAGTGCTGCAGGCTCAGGGGGGCTTGGGAGGA
  GCTGTTCTGCTCCCTGTCAGCTTCCAGGAGGGACGGCGGGCGTCGGACACCTCACTGACT
  CAAGGGCTGAAGGCCTTTCGGCAGCAGCTGAGGAAGACCACGCGTACCAAAGGGTTTCTG
  GGACTGAACAAAATCAAGGGGCTGGCTCGCCAGGTGTGCCAGGCCCCCGCCAGCCGGGCC
  AGCAGGGGCGGCCTGAGCCCCTTCCACGCCCCTGCACAGAGCCCAGGCCTGCACGGCGGC
  GCAGCCGGCAGCCGGGAGGGCTGGAGCCTGCTGGAGGAGGTGCTAGAGCAGCAGAGGCTG
  CTCCAGTTACAGCACCACCCGGCCGCTGCACCCGGCTGCTCCCAGGCCCCCCAGCCGGCC
  CCTGCCCCGTTTGTGATCGCCCCCTGTGATGGCCCTGGGGCTGCCCCGCTCCCCAGCACC
  CTCCTCACGTCGGGGCTCCCGCTGCTGCCGCCCCCACTCCTGCAGACCGGCGCGTCCCCG
  GTGGCCTCAGCGGCGCAGCTCCTGGACACACACCTGCACATTGGCACCGGCCCCACCGCC
  CTCCCCGCTGTGCCCCCACCACGCCTGGCCAGGCTGGCCCCAGGTTGTGAGCCCCTGGGG
  CTGCTGCAGGGGGACTGTGAGATGGAGGACCTGATGCCCTGCTCCCTAGGCACGTTTGTC
  CTGGTGCAGTGA
  
  Clone variation with respect to NM_173354.3
  167 a=>t;168 a=>g;1785 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_173354 unedited
  ACGCCGTTTGAGCAATGGGCGGTAGGCGCTGTACGGCTGTGGAGGTCTATATAAGCAGAGCTCATTTAGG
  CTGACACTATAGAATACAAGCTACTATGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCCGGAGGC
  AGCAGCAGCGGCGGCGGCAGCCGGAGCAGTAGGCACCCGAGCAGCGCCAGCGGCCGAGCGGGCGGCTTCC
  TGGCCTGGGCGCTCCGGTGGCGGCGGAGGTGCGCGCGGAGCCATGGTTATCATGTCGGAGTTCAGCGCGG
  ACCCCGCGGGCCAGGGTCAGGGCCAGCAGAAGCCCCTCCGGGTGGGTTTTTACGACATCGAGCGGACCCT
  GGGCAAAGGCAACTTCGCGGTGGTGAAGCTGGCGCGGCATCGAGTCACCAAAACGCAGGTTGCAATAATG
  ATAATTGATAAAACACGATTAGATTCAAGCAATTTGGAGAAAATCTATCGTGAGGTTCAGCTGATGAAGC
  TTCTGAACCATCCACACATCATAAAGCTTTACCAGGTTATGGAAACAAAGGACATGCTTTACATCGTCAC
  TGATTTGCTAAAAATGGAGAAATGTTTGATTATTTGACTTCCACGGGCACTGAGTGAGACCGAGGCGCGA
  GAGTTCTGGCAAATCCTTGTCGGCCGGTGGGAGTACTGTCACCGACATCCACATCGTCCACCGGGACCTC
  AGAACCGAAGACCTCTGCTGAATGCAACATGAACTCAGGCTGCGATTTTGAATTTGGAATTTCTACAGTC
  AGGAAAGCCCTCTGTCCTTTGGGTGGGGGAGCCCCCGTATGCCGCCCGGGAGTCTTTGAGGGAAGAGATT
  AGAAGGCCCCACCTGAAACTCTGGAACCTGGCGTGTGTGCTGACGTCTGATCACGTCTCCCTCGCATGGC
  TACTGGCAGCTGAACCGGTCTCTGAGCCATTCGCCATCGA
  
  Kinase domain sequence

  >SC323562 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSTTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTA
  TAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCCGGAGGCAGCAGCAGCG
  GCGGCGGCAGCCGGAGCAGTAGGCACCCGAGCAGCGCCAGCGGCCGAGCGGGCGGCTTCCTGGCCTGGGC
  GCTCCGGTGGCGGCGGAGGTGCGCGCGGAGCCATGGTTATCATGT
  
• Restriction Sites: Please inquire
• ACCN: NM_173354
• ORF Size: 2352 bp
• Insert Size: 4500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_173354.2, NP_775490.1
• RefSeq Size: 4723
• RefSeq ORF: 2352
• Locus ID: 150094
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a serine/threonine protein kinase that contains a ubiquitin-associated (UBA) domain. The encoded protein is a member of the adenosine monophosphate-activated kinase (AMPK) subfamily of kinases that play a role in conserved signal transduction pathways. A mutation in this gene is associated with early infantile epileptic encephalopathy 30. [provided by RefSeq, Nov 2016]