MOK protein kinase (MOK) (NM_014226) Human Untagged Clone

CAT#: SC323622

MOK (untagged)-Kinase deficient mutant (K33M) of Human renal tumor antigen (RAGE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MOK
• Synonyms: RAGE; RAGE-1; RAGE1; STK30
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014226, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGAACTATAAAGCAATTGGCAAAATAGGAGAGGGAACGTTTTCTGAAGTTATGAAGATGCAAAGCC
  TGAGAGATGGAAACTACTATGCATGTAAACAAATGAAGCAGCGCTTTGAAAGTATTGAGCAAGTCAACAA
  CCTACGAGAGATCCAAGCACTGAGGCGCCTGAATCCGCACCCAAACATTCTTATGTTGCATGAAGTGGTT
  TTTGACAGAAAATCTGGTTCTCTTGCACTAATATGTGAACTTATGGACATGAATATTTATGAGCTAATAC
  GAGGGAGAAGATACCCATTATCAGAAAAAAAAATTATGCACTATATGTACCAGTTATGTAAGTCCCTGGA
  TCATATTCACAGAAATGGAATATTTCACAGAGATGTAAAACCAGAAAATATACTAATAAAGCAGGATGTC
  CTGAAATTAGGGGACTTTGGCTCCTGCCGGAGTGTCTATTCCAAGCAGCCGTACACGGAATACATCTCCA
  CCCGCTGGTACCGGGCCCCGGAGTGTCTCCTCACTGATGGGTTCTACACGTACAAGATGGACCTGTGGAG
  CGCCGGCTGTGTGTTCTACGAGATCGCCAGTCTGCAGCCCCTCTTTCCTGGAGTAAATGAACTGGACCAA
  ATCTCAAAAATCCACGATGTCATCGGCACACCCGCTCAGAAGATCCTCACCAAGTTCAAACAGTCGAGAG
  CTATGAATTTTGATTTTCCTTTTAAAAAGGGATCAGGAATACCTCTACTAACAACCAATTTGTCCCCACA
  ATGCCTCTCCCTCCTGCACGCAATGGTGGCCTATGATCCCGATGAGAGAATCGCCGCCCACCAGGCCCTG
  CAGCACCCCTACTTCCAAGAACAGAGGAAAACAGAGAAGCGGGCTCTGGGCAGCCACAGAAAAGCTGGCT
  TTCCGGAGCACCCTGTGGCACCGGAACCACTCAGTAACAGCTGCCAGATTTCCAAGGAGGGCAGAAAGCA
  GAAACAGTCCCTAAAGCAAGAGGAGGACCGTCCCAAGAGACGAGGACCGGCCTATGTCATGGAACTGCCC
  AAACTAAAGCTTTCGGGAGTGGTCAGACTGTCGTCTTACTCCAGCCCCACGCTGCAGTCCGTGCTTGGAT
  CTGGAACAAATGGAAGAGTGCCGGTGCTGAGACCCTTGAAGTGCATCCCTGCGAGCAAGAAGACAGATCC
  GCAGAAGGACCTTAAGCCTGCCCCGCAGCAGTGTCGCCTGCCCACCATAGTGCGGAAAGGCGGAAGATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014226 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACAACATGGCGGCG
  GGATCTCTAACGCTCTCCTTCGAGGGACCACCACGGAGATCCTAGTGCGGGACCCCGCCTCAGGGAAGTG
  GAAAGCAGGGGGACAACCTTCCTGCTTCTTTCTTTTCCGTCCAGTGTCGGCAAGGGGTTGTCACCGGCTT
  CCGCATCCAAGATGAAGAACTATAAAGCAATTGGCAAAATAGGAGAGGGAACGTTTTCTGAAGTTATGAA
  GATGCAAAGCCTGAGAGATGGAAACTACTATGCATGTATGCAAATGAAGCAGCGCTTTGAAAGTGACAGA
  AAATCTGGTTCTCTTGCACTAATATGTGAACTTATGGACATGAATATTTATGAGCTAATACGAGGGAGAA
  GATACCCATTATCAGAAAAAAAAATTATGCACTATATGTACCAGTTATGTAAGTCCCTGGATCATATTCA
  CAGAAATGGAATATTTCACAGAGATGTAAAACCAGAAAATATACTAATAAAGCAGATGTCCTGAAATTAG
  GGGACTTTGCTCTGCCGGAGTTTTCTATTCCAAGCAGGCCGTACCACGAATACATCTCCACCCCGCTGTA
  CCGGGCCCCGGAGGGTTTCCTCCCTGATGGGTTCTAACCGTACAAAATGACCTGGGGACGCGCTTGTGGT
  TCCACAGAATCGCCAGTCTCGCAGCCCTCTTTCTGGATATGACTGACATTCAAATCCCAAGTTCATCGCC
  ACCCGTCAGAAATTCCACAGTCAACCCTCCTCTTGACCAAGTGCCATGATCGAGGAGAATCGCCCAAGCC
  TTGGAACTCATTCCACGGACGACGGCTGGCACAACGTC
  
  Kinase domain sequence

  >SC323622 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYYTKMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACAACATGGCGGCGGGAT
  CTCTAACGCTCTCCTTCGAGGGACCACCACGGAGATCCTAGTGCGGGACCCCGCCTCAGGGAAGTGGAAA
  GCAGGGGGACAACCTTCCTGCTTCTTTCTTTTCCGTCCAGTGTCG
  
• Restriction Sites: Please inquire
• ACCN: NM_014226
• Insert Size: 1770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014226.1, NP_055041.1
• RefSeq Size: 1954 bp
• RefSeq ORF: 1260 bp
• Locus ID: 5891
• Cytogenetics: 14q32.31
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene belongs to the MAP kinase superfamily. The gene was found to be regulated by caudal type transcription factor 2 (Cdx2) protein. The encoded protein, which is localized to epithelial cells in the intestinal crypt, may play a role in growth arrest and differentiation of cells of upper crypt and lower villus regions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Dec 2012]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).