MOK protein kinase (MOK) (NM_014226) Human Untagged Clone

CAT#: SC323622

MOK (untagged)-Kinase deficient mutant (K33M) of Human renal tumor antigen (RAGE)


  "NM_014226" in other vectors (7)

Reconstitution Protocol

USD 710.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MOK
Synonyms RAGE; RAGE-1; RAGE1; STK30
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_014226, the custom clone sequence may differ by one or more nucleotides


ATGAAGAACTATAAAGCAATTGGCAAAATAGGAGAGGGAACGTTTTCTGAAGTTATGAAGATGCAAAGCC
TGAGAGATGGAAACTACTATGCATGTAAACAAATGAAGCAGCGCTTTGAAAGTATTGAGCAAGTCAACAA
CCTACGAGAGATCCAAGCACTGAGGCGCCTGAATCCGCACCCAAACATTCTTATGTTGCATGAAGTGGTT
TTTGACAGAAAATCTGGTTCTCTTGCACTAATATGTGAACTTATGGACATGAATATTTATGAGCTAATAC
GAGGGAGAAGATACCCATTATCAGAAAAAAAAATTATGCACTATATGTACCAGTTATGTAAGTCCCTGGA
TCATATTCACAGAAATGGAATATTTCACAGAGATGTAAAACCAGAAAATATACTAATAAAGCAGGATGTC
CTGAAATTAGGGGACTTTGGCTCCTGCCGGAGTGTCTATTCCAAGCAGCCGTACACGGAATACATCTCCA
CCCGCTGGTACCGGGCCCCGGAGTGTCTCCTCACTGATGGGTTCTACACGTACAAGATGGACCTGTGGAG
CGCCGGCTGTGTGTTCTACGAGATCGCCAGTCTGCAGCCCCTCTTTCCTGGAGTAAATGAACTGGACCAA
ATCTCAAAAATCCACGATGTCATCGGCACACCCGCTCAGAAGATCCTCACCAAGTTCAAACAGTCGAGAG
CTATGAATTTTGATTTTCCTTTTAAAAAGGGATCAGGAATACCTCTACTAACAACCAATTTGTCCCCACA
ATGCCTCTCCCTCCTGCACGCAATGGTGGCCTATGATCCCGATGAGAGAATCGCCGCCCACCAGGCCCTG
CAGCACCCCTACTTCCAAGAACAGAGGAAAACAGAGAAGCGGGCTCTGGGCAGCCACAGAAAAGCTGGCT
TTCCGGAGCACCCTGTGGCACCGGAACCACTCAGTAACAGCTGCCAGATTTCCAAGGAGGGCAGAAAGCA
GAAACAGTCCCTAAAGCAAGAGGAGGACCGTCCCAAGAGACGAGGACCGGCCTATGTCATGGAACTGCCC
AAACTAAAGCTTTCGGGAGTGGTCAGACTGTCGTCTTACTCCAGCCCCACGCTGCAGTCCGTGCTTGGAT
CTGGAACAAATGGAAGAGTGCCGGTGCTGAGACCCTTGAAGTGCATCCCTGCGAGCAAGAAGACAGATCC
GCAGAAGGACCTTAAGCCTGCCCCGCAGCAGTGTCGCCTGCCCACCATAGTGCGGAAAGGCGGAAGATAA


>OriGene 5' read for mutant NM_014226 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACAACATGGCGGCG
GGATCTCTAACGCTCTCCTTCGAGGGACCACCACGGAGATCCTAGTGCGGGACCCCGCCTCAGGGAAGTG
GAAAGCAGGGGGACAACCTTCCTGCTTCTTTCTTTTCCGTCCAGTGTCGGCAAGGGGTTGTCACCGGCTT
CCGCATCCAAGATGAAGAACTATAAAGCAATTGGCAAAATAGGAGAGGGAACGTTTTCTGAAGTTATGAA
GATGCAAAGCCTGAGAGATGGAAACTACTATGCATGTATGCAAATGAAGCAGCGCTTTGAAAGTGACAGA
AAATCTGGTTCTCTTGCACTAATATGTGAACTTATGGACATGAATATTTATGAGCTAATACGAGGGAGAA
GATACCCATTATCAGAAAAAAAAATTATGCACTATATGTACCAGTTATGTAAGTCCCTGGATCATATTCA
CAGAAATGGAATATTTCACAGAGATGTAAAACCAGAAAATATACTAATAAAGCAGATGTCCTGAAATTAG
GGGACTTTGCTCTGCCGGAGTTTTCTATTCCAAGCAGGCCGTACCACGAATACATCTCCACCCCGCTGTA
CCGGGCCCCGGAGGGTTTCCTCCCTGATGGGTTCTAACCGTACAAAATGACCTGGGGACGCGCTTGTGGT
TCCACAGAATCGCCAGTCTCGCAGCCCTCTTTCTGGATATGACTGACATTCAAATCCCAAGTTCATCGCC
ACCCGTCAGAAATTCCACAGTCAACCCTCCTCTTGACCAAGTGCCATGATCGAGGAGAATCGCCCAAGCC
TTGGAACTCATTCCACGGACGACGGCTGGCACAACGTC
>SC323622 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYYTKMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACAACATGGCGGCGGGAT
CTCTAACGCTCTCCTTCGAGGGACCACCACGGAGATCCTAGTGCGGGACCCCGCCTCAGGGAAGTGGAAA
GCAGGGGGACAACCTTCCTGCTTCTTTCTTTTCCGTCCAGTGTCG
Restriction Sites Please inquire     
ACCN NM_014226
Insert Size 1770 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014226.1, NP_055041.1
RefSeq Size 1954 bp
RefSeq ORF 1260 bp
Locus ID 5891
Cytogenetics 14q32.31
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary 'This gene belongs to the MAP kinase superfamily. The gene was found to be regulated by caudal type transcription factor 2 (Cdx2) protein. The encoded protein, which is localized to epithelial cells in the intestinal crypt, may play a role in growth arrest and differentiation of cells of upper crypt and lower villus regions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Dec 2012]'
Transcript Variant: This variant (1) encodes the longer isoform (1).

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