Eph receptor A7 (EPHA7) (NM_004440) Human Untagged Clone
CAT#: SC323681
EPHA7 (untagged)-Kinase deficient mutant (K665M) of Human EPH receptor A7 (EPHA7)
"NM_004440" in other vectors (8)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | EPHA7 |
Synonyms | EHK-3; EHK3; EK11; HEK11 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC323681 sequence for NM_004440 edited (data generated by NextGen Sequencing)
ATGGTTTTTCAAACTCGGTACCCTTCATGGATTATTTTATGCTACATCTGGCTGCTCCGC TTTGCACACACAGGGGAGGCGCAGGCTGCGAAGGAAGTACTACTGCTGGATTCTAAAGCA CAACAAACAGAGTTGGAGTGGATTTCCTCTCCACCCAATGGGTGGGAAGAAATTAGTGGT TTGGATGAGAACTATACACCGATACGAACATACCAGGTGTGCCAAGTCATGGAGCCCAAC CAAAACAACTGGCTGCGGACTAACTGGATTTCCAAAGGCAATGCACAAAGGATTTTTGTA GAATTGAAATTCACCCTGAGGGATTGTAACAGTCTTCCTGGAGTACTGGGAACTTGCAAG GAAACATTTAATTTGTACTATTATGAAACAGACTATGACACTGGCAGGAATATAAGAGAA AACCTCTATGTAAAAATAGACACCATTGCTGCAGATGAAAGTTTTACCCAAGGTGACCTT GGTGAAAGAAAGATGAAGCTTAACACTGAGGTGAGAGAGATTGGACCTTTGTCCAAAAAG GGATTCTATCTTGCCTTTCAGGATGTAGGGGCTTGCATAGCTTTGGTTTCTGTCAAAGTG TACTACAAGAAGTGCTGGTCCATTATTGAGAACTTAGCTATCTTTCCAGATACAGTGACT GGTTCAGAATTTTCCTCTTTAGTCGAGGTTCGAGGGACATGTGTCAGCAGTGCAGAGGAA GAAGCGGAAAACGCCCCCAGGATGCACTGCAGTGCAGAAGGAGAATGGTTAGTGCCCATT GGAAAATGTATCTGCAAAGCAGGCTACCAGCAAAAAGGAGACACTTGTGAACCCTGTGGC CGTGGGTTCTACAAGTCTTCCTCTCAAGATCTTCAGTGCTCTCGTTGTCCAACTCACAGT TTTTCTGATAAAGAAGGCTCCTCCAGATGTGAATGTGAAGATGGGTATTACAGGGCTCCA TCTGACCCACCATACGTTGCATGCACAAGGCCTCCATCTGCACCACAGAACCTCATTTTC AACATCAACCAAACCACAGTAAGTTTGGAATGGAGTCCTCCTGCAGACAATGGGGGAAGA AACGATGTGACCTACAGAATATTGTGTAAGCGGTGCAGTTGGGAGCAGGGCGAATGTGTT CCCTGTGGGAGTAACATTGGATACATGCCCCAGCAGACTGGATTAGAGGATAACTATGTC ACTGTCATGGACCTGCTAGCCCACGCTAATTATACTTTTGAAGTTGAAGCTGTAAATGGA GTTTCTGACTTAAGCCGATCCCAGAGGCTCTTTGCTGCTGTCAGTATCACCACTGGTCAA GCAGCTCCCTCGCAAGTGAGCGGAGTAATGAAGGAGAGAGTACTGCAGCGGAGTGTCGAG CTTTCCTGGCAGGAACCAGAGCATCCCAATGGAGTCATCACAGAATATGAAATCAAGTAT TACGAGAAAGATCAAAGGGAACGGACCTACTCAACAGTAAAAACCAAGTCTACTTCAGCC TCCATTAATAATCTGAAACCAGGAACAGTGTATGTTTTCCAGATTCGGGCTTTTACTGCT GCTGGTTATGGAAATTACAGTCCCAGACTTGATGTTGCTACACTAGAGGAAGCTACAGGT AAAATGTTTGAAGCTACAGCTGTCTCCAGTGAACAGAATCCTGTTATTATCATTGCTGTG GTTGCTGTAGCTGGGACCATCATTTTGGTGTTCATGGTCTTTGGCTTCATCATTGGGAGA AGGCACTGTGGTTATAGCAAAGCTGACCAAGAAGGCGATGAAGAGCTTTACTTTCATTNN NNNNNNNNNNGCACCAAAACCTACATTGACCCTGAAACCTATGAGGACCCAAATAGAGCT GTCCATCAATTCGCCAAGGAGCTAGATGCCTCCTGTATTAAAATTGAGCGTGTGATTGGT GCAGGAGAATTCGGTGAAGTCTGCAGTGGCCGTTTGAAACTTCCAGGGAAAAGAGATGTT GCAGTAGCCATAAAAACCCTGAAAGTTGGTTACACAGAAAAACAAAGGAGAGACTTTTTG TGTGAAGCAAGCATCATGGGGCAGTTTGACCACCCAAATGTTGTCCATTTGGAAGGGGTT GTTACAAGAGGGAAACCAGTCATGATAGTAATAGAGTTCATGGAAAATGGAGCCCTAGAT GCATTTCTCAGGAAACATGATGGGCAATTTACAGTCATTCAGTTAGTAGGAATGCTGAGA GGAATTGCTGCTGGAATGAGATATTTGGCTGATATGGGATATGTTCACAGGGACCTTGCA GCTCGCAATATTCTTGTCAACAGCAATCTCGTTTGTAAAGTGTCAGATTTTGGCCTGTCC CGAGTTATAGAGGATGATCCAGAAGCTGTCTATACAACTACTGGTGGAAAAATTCCAGTA AGGTGGACAGCACCCGAAGCCATCCAGTACCGGAAATTCACATCAGCCAGTGATGTATGG AGCTATGGAATAGTCATGTGGGAAGTTATGTCTTATGGAGAAAGACCTTATTGGGACATG TCAAATCAAGATGTTATAAAAGCAATAGAAGAAGGTTATCGTTTACCAGCACCCATGGAC TGCCCAGCTGGCCTTCACCAGCTAATGTTGGATTGTTGGCAAAAGGAGCGTGCTGAAAGG CCAAAATTTGAACAGATAGTTGGAATTCTAGACAAAATGATTCGAAACCCAAATAGTCTG AAAACTCCCCTGGGAACTTGTAGTAGGCCAATAAGCCCTCTTCTGGATTAAAACACTCCT GATTTCACTACCTTTTGTTCAGTTGGAGAATGGCTACAAGCTATTAAGATGGAAAGATAT AAAGATAATTTCACGGCAGCTGGCTACAATTCCCTTGAATCAGTAGCCAGGATGACTATT GAGGATGTGATGAGTTTAGGGATCACACTGGTTGGTCATCAAAAGAAAATCATGAGCAGC ATTCAGACTATGAGAGCACAAATGCTACATTTACATGGAACTGGCATTCAAGTGTGA Clone variation with respect to NM_004440.3 198 c=>a;981 g=>a;1341 t=>c;1799 t=>n;1800 t=>n;1801 a=>n;1802 a=>n;1803 a=>n;1804 t=>n;1805 t=>n;1806 t=>n;1807 c=>n;1808 c=>n;1809 a=>n;1810 g=>n;2076 g=>a;2749 c=>t >OriGene 5' read for mutant NM_004440 unedited
CCCGCCGTCTCAGCAATGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAAACACGGTGC GAGCGAACAGGAGTGGGGGGGAAATTAAAAAAAGCTAAACGTGGAGCAGCCGATCGGGGACCGAGAAGGG GAATCGATGCAAGGAGCACAATAAAACAAAAGCTACTTCGGAACAAACAGCATTTAAAAATCCACGACTC AAGATAACTGAAAACCTAAAAATAAAACCTGCTCATGCACCATGGTTTTTCAAACTCGGGTACCCTTCAT GGATTATTTTATGCTACATCTGGCTGCCTCCCGCTTTGCCCACACCCCAGGGGAAGCCCGCAAGGCTTGC CGAAGGAGGTAACTATACTGTGCTTGGATATTCTTAAAGCCACAACACAAACAGAGAGTTGGGAGGTGGG AATTCCCTCTCTCACACCCATGTGGGTGTGGGAAGAGAAATTAATTGGTTGGATTAGAAACACATATAAC CCGGATCAGAGAAATAACCAAGGGTGTGCACAGTCTCAGGGAGCCCCCACAAAAAAACTGGGCGGCGGGA CTATCTGGATTTTTCAAAGCGCAATGCACAAGAGATTTTGTGGAAAATGAAATTTTACCCGAGGGGGTGT ATCACACCTCCTCGTAGTGCAGTGGAAATGCGCGGAGACACATATATGGGTCTATGAAACCGACTATTAT GACTCGCGCGAGTATATAAGAAACATCTTGTGTAAAATAACACCTGTTGCGAGAAGATTTATCCCCGTGA CATGTGAGAGATAGACTACACCTAGTGAGAGAGATGAGCCTCTGTCAAGAGTCATCTGCTCTCAGTGAAG GGTGCAGCACTGTAGTGCAGGATCACCAAGTCGTCCTCTTGACATACCTCTTCGGACCTGACGCTGCAGA TCCTATTCCAGTCCTGGCAA >SC323681 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CGKKCAWTGGTGCAGGAGATTCGGTGAGTCTGCAGTGGCCGTTTGAACTTCCAGGGAAAAGAGATGTTGC AGTAGCCATAATGACCCTGAAAGTTGGTTACACAGAAAAACAAAGGAGAGACTTTTTGTGTGAAGCAAGC ATCATGGGGCAGTTTGACCACCCAAATGTTGTCCATTTGGAAGGGGTTGTTACAAGAGGGAAACCAGTCA TGATAGTAATAGAGTTCATGGAAAATGGAGCCCTAGATGCATTTC |
Restriction Sites | Please inquire |
ACCN | NM_004440 |
Insert Size | 6600 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004440.2, NP_004431.1 |
RefSeq Size | 5229 bp |
RefSeq ORF | 2997 bp |
Locus ID | 2045 |
Cytogenetics | 6q16.1 |
Domains | pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3 |
Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
Protein Pathways | Axon guidance |
Gene Summary | 'This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Increased expression of this gene is associated with multiple forms of carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]' Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC317153 | EPHA7 (untagged)-Human EPH receptor A7 (EPHA7) |
USD 760.00 |
|
RC226293 | EPHA7 (Myc-DDK-tagged)-Human EPH receptor A7 (EPHA7) |
USD 420.00 |
|
RC600044 | EPHA7 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor A7, Signal peptide (1-27) plus EC domain (28-555) |
USD 660.00 |
|
RG226293 | EPHA7 (GFP-tagged) - Human EPH receptor A7 (EPHA7) |
USD 460.00 |
|
RC226293L1 | Lenti ORF clone of Human EPH receptor A7 (EPHA7), Myc-DDK-tagged |
USD 620.00 |
|
RC226293L2 | Lenti ORF clone of Human EPH receptor A7 (EPHA7), mGFP tagged |
USD 620.00 |
|
RC226293L3 | Lenti ORF clone of Human EPH receptor A7 (EPHA7), Myc-DDK-tagged |
USD 620.00 |
|
RC226293L4 | Lenti ORF clone of Human EPH receptor A7 (EPHA7), mGFP tagged |
USD 620.00 |
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