Eph receptor A7 (EPHA7) (NM_004440) Human Tagged ORF Clone

CAT#: RC226293

EPHA7 (Myc-DDK-tagged)-Human EPH receptor A7 (EPHA7)

Product Images

Western blot validation of overexpression lysate (Cat# LY429202) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226293 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EPHA7 protein (Cat# TP326293). The protein was produced from HEK293T cells transfected with EPHA7 cDNA clone (Cat# RC226293) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EPHA7
• Synonyms: EHK-3; EHK3; EK11; HEK11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226293 representing NM_004440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTTCAAACTCGGTACCCTTCATGGATTATTTTATGCTACATCTGGCTGCTCCGCTTTGCACACA
  CAGGGGAGGCGCAGGCTGCGAAGGAAGTACTACTGCTGGATTCTAAAGCACAACAAACAGAGTTGGAGTG
  GATTTCCTCTCCACCCAATGGGTGGGAAGAAATTAGTGGTTTGGATGAGAACTATACCCCGATACGAACA
  TACCAGGTGTGCCAAGTCATGGAGCCCAACCAAAACAACTGGCTGCGGACTAACTGGATTTCCAAAGGCA
  ATGCACAAAGGATTTTTGTAGAATTGAAATTCACCCTGAGGGATTGTAACAGTCTTCCTGGAGTACTGGG
  AACTTGCAAGGAAACATTTAATTTGTACTATTATGAAACAGACTATGACACTGGCAGGAATATAAGAGAA
  AACCTCTATGTAAAAATAGACACCATTGCTGCAGATGAAAGTTTTACCCAAGGTGACCTTGGTGAAAGAA
  AGATGAAGCTTAACACTGAGGTGAGAGAGATTGGACCTTTGTCCAAAAAGGGATTCTATCTTGCCTTTCA
  GGATGTAGGGGCTTGCATAGCTTTGGTTTCTGTCAAAGTGTACTACAAGAAGTGCTGGTCCATTATTGAG
  AACTTAGCTATCTTTCCAGATACAGTGACTGGTTCAGAATTTTCCTCTTTAGTCGAGGTTCGAGGGACAT
  GTGTCAGCAGTGCAGAGGAAGAAGCGGAAAACGCCCCCAGGATGCACTGCAGTGCAGAAGGAGAATGGTT
  AGTGCCCATTGGAAAATGTATCTGCAAAGCAGGCTACCAGCAAAAAGGAGACACTTGTGAACCCTGTGGC
  CGTGGGTTCTACAAGTCTTCCTCTCAAGATCTTCAGTGCTCTCGTTGTCCAACTCACAGTTTTTCTGATA
  AAGAAGGCTCCTCCAGATGTGAATGTGAAGATGGGTATTACAGGGCTCCATCTGACCCACCATACGTTGC
  ATGCACAAGGCCTCCATCTGCACCACAGAACCTCATTTTCAACATCAACCAAACCACAGTAAGTTTGGAA
  TGGAGTCCTCCTGCAGACAATGGGGGAAGAAACGATGTGACCTACAGAATATTGTGTAAGCGGTGCAGTT
  GGGAGCAGGGCGAATGTGTTCCCTGTGGGAGTAACATTGGATACATGCCCCAGCAGACTGGATTAGAGGA
  TAACTATGTCACTGTCATGGACCTGCTAGCCCACGCTAATTATACTTTTGAAGTTGAAGCTGTAAATGGA
  GTTTCTGACTTAAGCCGATCCCAGAGGCTCTTTGCTGCTGTCAGTATCACCACTGGTCAAGCAGCTCCCT
  CGCAAGTGAGCGGAGTAATGAAGGAGAGAGTACTGCAGCGGAGTGTCGAGCTTTCCTGGCAGGAACCAGA
  GCATCCCAATGGAGTCATCACAGAATATGAAATCAAGTATTACGAGAAAGATCAAAGGGAACGGACCTAC
  TCAACAGTAAAAACCAAGTCTACTTCAGCCTCCATTAATAATCTGAAACCAGGAACAGTGTATGTTTTCC
  AGATTCGGGCTTTTACTGCTGCTGGTTATGGAAATTACAGTCCCAGACTTGATGTTGCTACACTAGAGGA
  AGCTACAGGTAAAATGTTTGAAGCTACAGCTGTCTCCAGTGAACAGAATCCTGTTATTATCATTGCTGTG
  GTTGCTGTAGCTGGGACCATCATTTTGGTGTTCATGGTCTTTGGCTTCATCATTGGGAGAAGGCACTGTG
  GTTATAGCAAAGCTGACCAAGAAGGCGATGAAGAGCTTTACTTTCATTTTAAATTTCCAGGCACCAAAAC
  CTACATTGACCCTGAAACCTATGAGGACCCAAATAGAGCTGTCCATCAATTCGCCAAGGAGCTAGATGCC
  TCCTGTATTAAAATTGAGCGTGTGATTGGTGCAGGAGAATTCGGTGAAGTCTGCAGTGGCCGTTTGAAAC
  TTCCAGGGAAAAGAGATGTTGCAGTAGCCATAAAAACCCTGAAAGTTGGTTACACAGAAAAACAAAGGAG
  AGACTTTTTGTGTGAAGCAAGCATCATGGGGCAGTTTGACCACCCGAATGTTGTCCATTTGGAAGGGGTT
  GTTACAAGAGGGAAACCAGTCATGATAGTAATAGAGTTCATGGAAAATGGAGCCCTAGATGCATTTCTCA
  GGAAACATGATGGGCAATTTACAGTCATTCAGTTAGTAGGAATGCTGAGAGGAATTGCTGCTGGAATGAG
  ATATTTGGCTGATATGGGATATGTTCACAGGGACCTTGCAGCTCGCAATATTCTTGTCAACAGCAATCTC
  GTTTGTAAAGTGTCAGATTTTGGCCTGTCCCGAGTTATAGAGGATGATCCAGAAGCTGTCTATACAACTA
  CTGGTGGAAAAATTCCAGTAAGGTGGACAGCACCCGAAGCCATCCAGTACCGGAAATTCACATCAGCCAG
  TGATGTATGGAGCTATGGAATAGTCATGTGGGAAGTTATGTCTTATGGAGAAAGACCTTATTGGGACATG
  TCAAATCAAGATGTTATAAAAGCAATAGAAGAAGGTTATCGTTTACCAGCACCCATGGACTGCCCAGCTG
  GCCTTCACCAGCTAATGTTGGATTGTTGGCAAAAGGAGCGTGCTGAAAGGCCAAAATTTGAACAGATAGT
  TGGAATTCTAGACAAAATGATTCGAAACCCAAATAGTCTGAAAACTCCCCTGGGAACTTGTAGTAGGCCA
  ATAAGCCCTCTTCTGGATCAAAACACTCCTGATTTCACTACCTTTTGTTCAGTTGGAGAATGGCTACAAG
  CTATTAAGATGGAAAGATATAAAGATAATTTCACGGCAGCTGGCTACAATTCCCTTGAATCAGTAGCCAG
  GATGACTATTGAGGATGTGATGAGTTTAGGGATCACACTGGTTGGTCATCAAAAGAAAATCATGAGCAGC
  ATTCAGACTATGAGAGCACAAATGCTACATTTACATGGAACTGGCATTCAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226293 representing NM_004440
  Red=Cloning site Green=Tags(s)
  
  MVFQTRYPSWIILCYIWLLRFAHTGEAQAAKEVLLLDSKAQQTELEWISSPPNGWEEISGLDENYTPIRT
  YQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTCKETFNLYYYETDYDTGRNIRE
  NLYVKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIIE
  NLAIFPDTVTGSEFSSLVEVRGTCVSSAEEEAENAPRMHCSAEGEWLVPIGKCICKAGYQQKGDTCEPCG
  RGFYKSSSQDLQCSRCPTHSFSDKEGSSRCECEDGYYRAPSDPPYVACTRPPSAPQNLIFNINQTTVSLE
  WSPPADNGGRNDVTYRILCKRCSWEQGECVPCGSNIGYMPQQTGLEDNYVTVMDLLAHANYTFEVEAVNG
  VSDLSRSQRLFAAVSITTGQAAPSQVSGVMKERVLQRSVELSWQEPEHPNGVITEYEIKYYEKDQRERTY
  STVKTKSTSASINNLKPGTVYVFQIRAFTAAGYGNYSPRLDVATLEEATGKMFEATAVSSEQNPVIIIAV
  VAVAGTIILVFMVFGFIIGRRHCGYSKADQEGDEELYFHFKFPGTKTYIDPETYEDPNRAVHQFAKELDA
  SCIKIERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKVGYTEKQRRDFLCEASIMGQFDHPNVVHLEGV
  VTRGKPVMIVIEFMENGALDAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSNL
  VCKVSDFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDM
  SNQDVIKAIEEGYRLPAPMDCPAGLHQLMLDCWQKERAERPKFEQIVGILDKMIRNPNSLKTPLGTCSRP
  ISPLLDQNTPDFTTFCSVGEWLQAIKMERYKDNFTAAGYNSLESVARMTIEDVMSLGITLVGHQKKIMSS
  IQTMRAQMLHLHGTGIQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004440
• ORF Size: 2994 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004440.1, NM_004440.2, NM_004440.3, NP_004431.1
• RefSeq ORF: 2997 bp
• Locus ID: 2045
• Cytogenetics: 6q16.1
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• MW: 111.9 kDa
• Gene Summary: 'This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Increased expression of this gene is associated with multiple forms of carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]'