NEK11 (NM_024800) Human Untagged Clone

CAT#: SC323687

NEK11 (untagged)-Kinase deficient mutant (K61M) of Human NIMA (never in mitosis gene a)- related kinase 11 (NEK11), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEK11
• Synonyms: FLJ23495
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323687 sequence for NM_024800 edited (data generated by NextGen Sequencing)
  ATGCTGAAATTCCAAGAGGCAGCTAAGTGTGTGAGTGGATCAACAGCCATTTCCACTTAT
  CCAAAGACCTTGATTGCAAGAAGATACGTGCTTCAACAAAAACTTGGCAGTGGAAGTTTT
  GGAACTGTCTATCTGGTTTCAGACAAGAAAGCCAAACGAGGAGAGGAATTAAAGGTACTT
  ATGGAAATATCTGTTGGAGAACTAAATCCAAATGAAACTGTACAGGCCAATTTGGAAGCC
  CAACTCCTCTCCAAGCTGGACCACCCAGCCATTGTCAAGTTCCATGCAAGTTTTGTGGAG
  CAAGATAATTTCTGCATTATCACGGAGTACTGTGAGGGCCGAGATCTGGACGATAAAATT
  CAGGAATATAAACAAGCTGGAAAAATCTTTCCAGAAAATCAAATAATAGAATGGTTTATC
  CAGCTGCTGCTGGGAGTTGACTACATGCATGAGAGGAGGATACTTCATCGAGACTTAAAG
  TCAAAGAATGTATTTCTGAAAAATAATCTCCTTAAAATTGGAGATTTTGGAGTTTCTCGA
  CTTCTAATGGGATCCTGTGACCTGGCCACAACTTTAACTGGAACTCCCCATTATATGAGT
  CCTGAGGCTCTGAAACACCAAGGCTATGACACAAAGTCGGACATCTGGTCACTGGCATGC
  ATTTTGTATGAGATGTGCTGCATGAATCATGCATTCGCTGGCTCCAATTTCTTATCCATT
  GTTTTAAAAATTGTTGAAGGTGACACACCTTCTCTCCCTGAGAGATATCCAAAAGAACTA
  AATGCCATCATGGAAAGCATGTTGAACAAGAATCCTTCATTAAGACCATCTGCTATCGAA
  ATTTTAAAAATCCCTTACCTTGATGAGCAGCTACAGAACCTAATGTGTAGATATTCAGAA
  ATGACTCTGGAAGACAAAAATTTGGATTGTCAGAAGGAGGCTGCTCATATAATTAATGCC
  ATGCAAAAAAGGATCCACCTGCAGACTCTGAGGGCACTGTCAGAAGTACAGAAAATGACG
  CCAAGAGAAAGGATGCGGCTGAGGAAGCTCCAGGCGGCTGATGAGAAAGCCAGGAAGCTG
  AAAAAGATTGTGGAAGAAAAATATGAAGAAAATAGCAAACGAATGCAAGAATTGAGATCT
  CGGAACTTTCAGCAGCTGAGTGTTGATGTACTCCATGAAAAAACACATTTAAAAGGAATG
  GAAGAAAAGGAGGAGCAACCTGAGGGAAGACTTTCTTGTTCACCCCAGGACGAGGATGAA
  GAGAGGTGGCAAGGCAGGGAAGAGGAATCTGATGAACCAACTTTAGAGAACCTGCCTGAG
  TCTCAGCCTATTCCTTCCATGGACCTCCACGAACTTGAATCAATTGTAGAGGATGCCACA
  TCTGACCTTGGATACCATGAGATCCCAGAAGACCCACTTGTGGCTGAAGAGTACTACGCT
  GATGCATTTGATTCCTATTGTGTAGAGAGTGATGAGGAGGAAGAAGAAATAGCGTTAGAA
  AGACCAGAGAAAGAAATCAGGAATGAGGGATCCCAGCCTGCTTACAGAACAAACCAACAG
  GACAGTGATATCGAAGCGTTGGCCAGGTGTTTGGAAAATGTCCTGGGTTGCACTTCTCTA
  GACACAAAGACCATCACCACCATGGCTGAAGACATGTCCCCAGGACCACCAATTTTCAAC
  AGTGTGATGGCCAGGACCAAGATGAAACGCATGAGGGAATCAGCCATGCAGAAGCTGGGG
  ACAGAAGTATTTGAAGAGGTCTATAATTACCTCAAGAGAGCAAGGCATCAGAATGCTAGC
  GAAGCAGAGATCCGCGAGTGTTTGGAAAAAGTGGTGCCTCAAGCCAGCGACTGTTTTGAA
  GTGGACCAGCTCCTGTACTTTGAAGAGCAGTTGCTGATCACGATGGGAAAAGAACCTACT
  CTCCAGAACCATCTCTAG
  
  Clone variation with respect to NM_024800.4
  182 a=>t;1463 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_024800 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTTAATCTCATCTT
  TAAAATAAGGAGAATTACTGAGTGACCTGAAGGACCCTTTTCAGCTGGAAAGTCTGAACTGACCAACACT
  GGATGAATTTGACCATTTCTTAGGAGACTGGAATGTTAAGTTTCTATAAATGAATGAACCAGTTCTCTCT
  TGTTTGGAGCAATGCTGAAATTCCAAGAGGCAGCTAAGTGTGTGAGTGGATCAACAGCCATTTTCCACTT
  ATCCCAAAGACCTTGATTTGCAAGAAGATTACGTGCTTCACCAAAAAACTTGGCAGTTGGAAAGTTTTTG
  GACCTGGTCCTATCTGTTTTCAGAACAAGAAAAGCAAAACGGAGGAAGAGGATTTAAAGGTACTTTATGA
  AAAATATCTTGTGGAGGAACTAAAATCAAAATGAAAACTGGACCGGGCCATTTGGAAGCCCCACCTCTCT
  TTCCAACCGGGCACCCCCAGCCAATGGGCCAGGTTCCATTCCAGGTTTGTGGGGAACAAGATTATTTCGG
  CTATACCCGAAACCGGTAGGGGCCGAGTCTCGGGGAGATAAATTTCGGATTACAACTGGAAAACTTCCGA
  ATACAAATAGAAGGGTAACCCTCGCTGGATTGCACTCTCGAGAGAGAGACTCTCTCAGACTTAGTCCAGA
  GAGTTTTGAGATAATCCCTAATGGATTGAGTTTCGCATCTAGATCTGACTGCCACACTACTGATCCATAA
  GTCAGGCTGACAGTTACATGACTGACGAGCATGGGAGGTGCTGTATGCCTGTCAAACTTAACGTCAAGTG
  AGAAG
  
  Kinase domain sequence

  >SC323687 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CAACTTGGCAGTGGAGTTTGGACTGTCTATCTGGTTTCAGACAAGAAAGCCAAACGAGGAGAGGAATTAA
  AGGTACTTATGGAAATATCTGTTGGAGAACTAAATCCAAATGAAACTGTACAGGCCAATTTGGAAGCCCA
  ACTCCTCTCCAAGCTGGACCACCCAGCCATTGTCAAGTTCCATGCAAGTTTTGTGGAGCAAGATAATTTC
  TGCATTATCACGGAGTACTGTGAGGGCCGAGATCTGGACGATAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_024800
• ORF Size: 1938 bp
• Insert Size: 3000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_024800.2, NP_079076.2
• RefSeq Size: 2939
• RefSeq ORF: 1938
• Locus ID: 79858
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (1) encodes isoform (1), also known as NEK11L.