LATS1 (NM_004690) Human Untagged Clone

CAT#: SC323703

LATS1 (untagged)-Kinase deficient mutant (K734M) of Human LATS, large tumor suppressor, homolog 1 (Drosophila) (LATS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LATS1
• Synonyms: WARTS; wts
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004690, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGAGGAGTGAAAAGCCAGAAGGATATAGACAAATGAGGCCTAAGACCTTTCCTGCCAGTAACTATA
  CTGTCAGTAGCCGGCAAATGTTACAAGAAATTCGGGAATCCCTTAGGAATTTATCTAAACCATCTGATGC
  TGCTAAGGCTGAGCATAACATGAGTAAAATGTCAACCGAAGATCCTCGACAAGTCAGAAATCCACCCAAA
  TTTGGGACGCATCATAAAGCCTTGCAGGAAATTCGAAACTCTCTGCTTCCATTTGCAAATGAAACAAATT
  CTTCTCGGAGTACTTCAGAAGTTAATCCACAAATGCTTCAAGACTTGCAAGCTGCTGGATTTGATGAGGA
  TATGGTTATACAAGCTCTTCAGAAAACTAACAACAGAAGTATAGAAGCAGCAATTGAATTCATTAGTAAA
  ATGAGTTACCAAGATCCTCGACGAGAGCAGATGGCTGCAGCAGCTGCCAGACCTATTAATGCCAGCATGA
  AACCAGGGAATGTGCAGCAATCAGTTAACCGCAAACAGAGCTGGAAAGGTTCTAAAGAATCCTTAGTTCC
  TCAGAGGCATGGCCCGCCACTAGGAGAAAGTGTGGCCTATCATTCTGAGAGTCCCAACTCACAGACAGAT
  GTAGGAAGACCTTTGTCTGGATCTGGTATATCAGCATTTGTTCAAGCTCACCCTAGCAACGGACAGAGAG
  TGAACCCCCCACCACCACCTCAAGTAAGGAGTGTTACTCCTCCACCACCTCCAAGAGGCCAGACTCCCCC
  TCCAAGAGGTACAACTCCACCTCCCCCTTCATGGGAACCAAACTCTCAAACAAAGCGCTATTCTGGAAAC
  ATGGAATACGTAATCTCCCGAATCTCTCCTGTCCCACCTGGGGCATGGCAAGAGGGCTATCCTCCACCAC
  CTCTCAACACTTCCCCCATGAATCCTCCTAATCAAGGACAGAGAGGCATTAGTTCTGTTCCTGTTGGCAG
  ACAACCAATCATCATGCAGAGTTCTAGCAAATTTAACTTTCCATCAGGGAGACCTGGAATGCAGAATGGT
  ACTGGACAAACTGATTTCATGATACACCAAAATGTTGTCCCTGCTGGCACTGTGAATCGGCAGCCACCAC
  CTCCATATCCTCTGACAGCAGCTAATGGACAAAGCCCTTCTGCTTTACAAACAGGGGGATCTGCTGCTCC
  TTCGTCATATACAAATGGAAGTATTCCTCAGTCTATGATGGTGCCAAACAGAAATAGTCATAACATGGAA
  CTATATAACATTAGTGTACCTGGACTGCAAACAAATTGGCCTCAGTCATCTTCTGCTCCAGCCCAGTCAT
  CCCCGAGCAGTGGGCATGAAATCCCTACATGGCAACCTAACATACCAGTGAGGTCAAATTCTTTTAATAA
  CCCATTAGGAAATAGAGCAAGTCACTCTGCTAATTCTCAGCCTTCTGCTACAACAGTCACTGCAATTACA
  CCAGCTCCTATTCAACAGCCTGTGAAAAGTATGCGTGTATTAAAACCAGAGCTACAGACTGCTTTAGCAC
  CTACACACCCTTCTTGGATACCACAGCCAATTCAAACTGTTCAACCCAGTCCTTTTCCTGAGGGAACCGC
  TTCAAATGTGACTGTGATGCCACCTGTTGCTGAAGCTCCAAACTATCAAGGACCACCACCACCCTACCCA
  AAACATCTGCTGCACCAAAACCCATCTGTTCCTCCATACGAGTCAATCAGTAAGCCTAGCAAAGAGGATC
  AGCCAAGCTTGCCCAAGGAAGATGAGAGTGAAAAGAGTTATGAAAATGTTGATAGTGGGGATAAAGAAAA
  GAAACAGATTACAACTTCACCTATTACTGTTAGGAAAAACAAGAAAGATGAAGAGCGAAGGGAATCTCGT
  ATTCAAAGTTATTCTCCTCAAGCATTTAAATTCTTTATGGAGCAACATGTAGAAAATGTACTCAAATCTC
  ATCAGCAGCGTCTACATCGTAAAAAACAATTAGAGAATGAAATGATGCGGGTTGGATTATCTCAAGATGC
  CCAGGATCAAATGAGAAAGATGCTTTGCCAAAAAGAATCTAATTACATCCGTCTTAAAAGGGCTAAAATG
  GACAAGTCTATGTTTGTGAAGATAAAGACACTAGGAATAGGAGCATTTGGTGAAGTCTGTCTAGCAAGAA
  AAGTAGATACTAAGGCTTTGTATGCAACAAAAACTCTTCGAAAGAAAGATGTTCTTCTTCGAAATCAAGT
  CGCTCATGTTAAGGCTGAGAGAGATATCCTGGCTGAAGCTGACAATGAATGGGTAGTTCGTCTATATTAT
  TCATTCCAAGATAAGGACAATTTATACTTTGTAATGGACTACATTCCTGGGGGTGATATGATGAGCCTAT
  TAATTAGAATGGGCATCTTTCCAGAAAGTCTGGCACGATTCTACATAGCAGAACTTACCTGTGCAGTTGA
  AAGTGTTCATAAAATGGGTTTTATTCATAGAGATATTAAACCTGATAATATTTTGATTGATCGTGATGGT
  CATATTAAATTGACTGACTTTGGCCTCTGCACTGGCTTCAGATGGACACACGATTCTAAGTACTATCAGA
  GTGGTGACCATCCACGGCAAGATAGCATGGATTTCAGTAATGAATGGGGGGATCCCTCAAGCTGTCGATG
  TGGAGACAGACTGAAGCCATTAGAGCGGAGAGCTGCACGCCAGCACCAGCGATGTCTAGCACATTCTTTG
  GTTGGGACTCCCAATTATATTGCACCTGAAGTGTTGCTACGAACAGGATACACACAGTTGTGTGATTGGT
  GGAGTGTTGGTGTTATTCTTTTTGAAATGTTGGTGGGACAACCTCCTTTCTTGGCACAAACACCATTAGA
  AACACAAATGAAGGTTATCAACTGGCAAACATCTCTTCACATTCCACCACAAGCTAAACTCAGTCCTGAA
  GCTTCTGATCTTATTATTAAACTTTGCCGAGGACCCGAAGATCGCTTAGGCAAGAATGGTGCTGATGAAA
  TAAAAGCTCATCCATTTTTTAAAACAATTGACTTCTCCAGTGACCTGAGACAGCAGTCTGCTTCATACAT
  TCCTAAAATCACACACCCAACAGATACATCAAATTTTGATCCTGTTGATCCTGATAAATTATGGAGTGAT
  GATAACGAGGAAGAAAATGTAAATGACACTCTCAATGGATGGTATAAAAATGGAAAGCATCCTGAACATG
  CATTCTATGAATTTACCTTCCGAAGGTTTTTTGATGACAATGGCTACCCATATAATTATCCGAAGCCTAT
  TGAATATGAATACATTAATTCACAAGGCTCAGAGCAGCAGTCGGATGAAGATGATCAAAACACAGGCTCA
  GAGATTAAAAATCGCGATCTAGTATATGTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004690 unedited
  CCGCCCGTCTGAGCAATGGGCGGTAGGCGTGTACTGGACAGTGGAGGTCTATATAAGCAGAGCTCGTTTA
  GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGGCGGCGG
  CGGTGGCCTGGTGTTAAGGGGAGAGCCAGGTCCTTACGACCCCTGGGACGGGCCGCGCTGGCCCGCGGCA
  GCCCCCCCGTTCGTCTCCCCGCTCTGCCCCACCAGGGATACTTGGGGTTGCTGGGACGGACTCTGGCCGC
  CTCAGCGTCCGCCCTCAGGCCCGTGGCCGCTGTCCAGGAGCTCTGCTCTCCCCTCCAGAGTTAATTATTT
  ATATTGTAAAGAATTTTAACAGTCCTGGGGACTTCCTTGAAGCATCATTTTCACTTTTGCTCAGAAGAAA
  GCTCTGGATCTATCAAATAAAGAAGTCCTTCGTGTGGGCTACATATATAGATGTTTTCATGAAGAGGAGT
  GAAAAGCCAGAAGGATATAGACAAATGAGGCCTAAGACCTTTCCTGCCAGTAACTATACTGTCAGTAGCC
  GGCAAATGTTACAAGAAATTCGGGAATCCCTTAGGAATTTATCTAAACCATCTGATGCTGCTAAGGCTGA
  GCATAACATGAGTAAAATGTCAACCGAAGATCCTCGACAAGTCAGAAATCCACCCAAATTTGGGACGCAT
  CATAAGCCTTGCAGAAATTCGAAACTCTCTGCTTCCATTTGCAAATGAAACAAATTCTTCTCGGAGTACT
  TCAGAAGTTAATCCACAAATGCTTCAGGACTTGCAGCTGCTGGATTTGATGAGATATGGGTTATACAGCT
  CTTCAGAACTAACAACAGAAGTATAGAAGCAGCATTTGAATTCATTAGTAAAATGGAGTACCAGATCTCG
  ACGAGAGCGAATGCTGCAGCGCTGCGACCTATTTAATGCCAGCATGAAACCAGGGATGTTGCCCGCATCA
  GTTAG
  
  Kinase domain sequence

  >SC323703 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TYTCTCAGCATTTAATTCTTTATGGAGCAACATGTAGAAAATGTACTCAAATCTCATCAGCAGCGTCTAC
  ATCGTAAAAAACAATTAGAGAATGAAATGATGCGGCTGATTTAAACTTTTATGGTGTTGGAAGCTTTGCT
  GACTTCTGTAGTCTCCATTACTGTGTAGGTTGGATTATCTCAAGATGCCCAGGATCAAATGAGAAAGATG
  CTTTGCCAAAAAGAATCTAATTACATCCGTCTTAAAAGGGCTAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004690
• ORF Size: 3393 bp
• Insert Size: 4700
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_004690.2, NP_004681.1
• RefSeq Size: 4756
• RefSeq ORF: 3393
• Locus ID: 9113
• Domains: UBA, pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.