RAD18 (NM_020165) Human Untagged Clone

CAT#: SC323786

RAD18 (untagged)-Human RAD18 homolog (S. cerevisiae) (RAD18)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD18
• Synonyms: RNF73
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020165.2 GTGTTGGAGCGCCGGAGGCTAGTGGGTGGCTGACCCCCAGCATCCTCGAGAGCGACCATG
  GACTCCCTGGCCGAGTCTCGGTGGCCTCCGGGCCTGGCAGTCATGAAGACAATAGATGAT
  TTGCTGCGGTGTGGAATTTGCTTCGAGTATTTCAACATTGCAATGATAATACCTCAGTGT
  TCACATAACTACTGCTCTCTCTGTATAAGAAAATTTCTGTCCTATAAAACTCAGTGTCCA
  ACTTGCTGTGTGACTGTCACAGAGCCGGATCTGAAAAATAACCGCATATTAGATGAACTG
  GTAAAAAGCTTGAATTTTGCACGGAATCATCTGCTGCAGTTTGCTTTAGAGTCACCAGCC
  AAATCTCCTGCTTCTTCCTCTTCAAAGAATCTTGCTGTCAAAGTATATACTCCTGTAGCC
  TCCAGACAGTCTTTAAAGCAGGGGAGCAGGTTAATGGATAATTTCTTGATCAGAGAAATG
  AGTGGTTCTACATCAGAGTTGTTGATAAAAGAAAATAAAAGCAAATTCAGCCCTCAAAAA
  GAGGCGAGCCCTGCTGCAAAGACCAAAGAGACACGTTCTGTAGAAGAGATCGCTCCAGAT
  CCCTCAGAGGCTAAGCGTCCTGAGCCACCCTCGACATCCACTTTGAAACAAGTTACTAAA
  GTGGATTGTCCTGTTTGCGGGGTTAACATTCCAGAAAGTCACATTAATAAGCATTTAGAC
  AGCTGTTTATCACGCGAAGAGAAGAAGGAAAGCCTCAGAAGTTCTGTTCACAAAAGGAAG
  CCGCTGCCCAAAACTGTATATAATTTGCTCTCTGATCGTGATTTAAAGAAAAAGCTAAAA
  GAGCATGGATTATCTATTCAAGGAAATAAACAACAGCTCATTAAAAGGCACCAAGAATTT
  GTACACATGTACAATGCCCAATGCGATGCTTTGCATCCTAAATCAGCTGCTGAAATAGTT
  CAAGAAATCGAAAATATAGAGAAGACTAGGATGCGTCTTGAAGCTAGTAAACTCAATGAA
  AGTGTAATGGTTTTTACAAAGGACCAAACAGAAAAGGAAATAGATGAAATCCACAGTAAA
  TATCGTAAAAAACATAAGAGTGAATTTCAGCTTCTGGTGGATCAGGCTAGAAAAGGATAC
  AAGAAAATTGCTGGAATGTCACAAAAAACAGTAACAATAACAAAAGAAGATGAATCTACA
  GAAAAGCTATCTTCTGTATGCATGGGACAGGAAGATAATATGACCTCAGTAACAAACCAC
  TTTTCTCAATCAAAGCTGGACTCCCCAGAGGAATTGGAACCTGACAGAGAAGAGGATTCT
  TCTAGCTGTATTGATATTCAAGAAGTTCTTTCTTCATCAGAATCAGATTCATGCAATAGT
  TCCAGTTCAGACATCATAAGAGATCTTTTAGAAGAAGAGGAAGCCTGGGAAGCATCACAT
  AAAAACGATCTTCAAGACACAGAAATAAGTCCAAGACAGAATCGCCGCACAAGAGCCGCT
  GAAAGTGCTGAGATTGAACCAAGAAACAAGCGTAATAGGAATTAATGTGGGCTTTTGCTG
  ACTTTTCAAATGCATTGATTAGAATACCGTACTTTTGGTTGCCACAGATAGATTTTCTAT
  TTATAAATGCCCAAGGAAAGATGCTAAATTCTAAATATTACGGTTAGCTGATATTCATTC
  TTTTCTGCTTTTCCAGAGGGGAAAAATGTTACAAAATATCCTTACTTGGTCAGTTGCCTC
  CTGCCTCTAAAACATCTCTCTCTAAAAATACTGACATTTCACACAGGTACCAGCTTTGCA
  GAGGAGGTAGACTCTTTGGCACTTTGGCACAGGGATTTGGTTTGGTTTGGTTTGGTTTGA
  TAATTAAATTTCAGAATGTCAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020165.2 unedited
  GACGTTGTATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTCCGG
  AATTCCCGGGATATCGTCGACCCACGCGTCCGGTGTTGGAGCGCCGGAGGCTAGTGGGTG
  GCTGACCCCCAGCATCCTCGAGAGCGACCATGGACTCCCTGGCCGAGTCTCGGTGGCCTC
  CGGGCCTGGCAGTCATGAAGACAATAGATGATTTGCTGCGGTGTGGAATTTGCTTCGAGT
  ATTTCAACATTGCAATGATAATACCTCAGTGTTCACATAACTACTGCTCTCTCTGTATAA
  GAAAATTTCTGTCCTATAAAACTCAGTGTCCAACTTGCTGTGTGACTGTCACAGAGCCGG
  ATCTGAAAAATAACCGCATATTAGATGAACTGGTAAAAAGCTTGAATTTTGCACGGAATC
  ATCTGCTGCAGTTTGCTTTAGAGTCACCAGCCAAATCTCCTGCTTCTTCCTCTTCAAAGA
  ATCTTGCTGTCAAAGTATATACTCCTGTAGCCTCCAGACAGTCTTTAAAGCAGGGGAGCA
  GGTTAATGGATAATTTCTTGATCAGAGAAATGAGTGGTTCTACATCAGAGTTGTTGATAA
  AAGAAAATAAAAGCAAATTCAGCCCTCAAAAAGAGGCGAGCCCTGCTGCAAAGACCAAAG
  AGACACGTTCTGTAGAAGAGATCGCTCCAGATCCCTCAGAGGCTAAGCGTCCTGAGCCAC
  CCTCGACATCCACTTTGAAACAGTTACTAAAGTGGATTGTCCTGTTTGCGGGGTTAACAT
  TCCAGAAAGTCACATTAATAAGCATTTAGAACAGCTTGTTTATCACCCCGAAGAGAGAGA
  AAGCCTCAGAGTTCTGTTCACAAAAAGAGCCGCTGCCAAACTGTATATATTTGCTCTCTG
  ATCGTGATTAAGGAAAAGGCTAAAGAGCATGATTATTCTATCCAGGAATAAACAACAGCC
  TTCAATAAGGGCACCAGGAATGGGAG
• Restriction Sites: ECoRI-NOT
• ACCN: NM_020165
• ORF Size: 1488 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_020165.2, NP_064550.2
• RefSeq Size: 3053
• RefSeq ORF: 1488
• Locus ID: 56852
• Domains: RING, SAP, ZnF_Rad18
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]