NFIB (NM_005596) Human Untagged Clone

CAT#: SC323795

NFIB (untagged)-Human nuclear factor I/B (NFIB), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFIB
• Synonyms: CTF; HMGIC/NFIB; MACID; NF-I/B; NF1-B; NFI-B; NFI-RED; NFIB2; NFIB3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005596.2 GACTGGGTTTGTTGTGAAGTTGCGGTGATCCAGCGCAGAGCCCCGTCCTGATTGATCGCA
  TCGCGGGGCTCAGATGACTGTAAAATGAATAGATGAAATTCTTGCTTCTCGAAGATTTTC
  TTGGGCATCTCCCGGAAAGTGCGTTTTAAGGCGAAGTCATGATGTATTCTCCCATCTGTC
  TCACTCAGGATGAATTTCACCCATTCATCGAGGCACTTCTTCCACATGTCCGTGCAATTG
  CCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAGTACTTTAAAAAGCATGAGAAGC
  GAATGTCAAAGGATGAAGAAAGAGCAGTCAAAGATGAGCTTCTCAGTGAAAAGCCTGAAA
  TCAAACAGAAGTGGGCATCCAGGCTCCTTGCCAAACTGCGCAAAGATATTCGCCAGGAGT
  ATCGAGAGGACTTTGTGCTCACCGTGACTGGCAAGAAGCACCCGTGCTGTGTCTTATCCA
  ATCCCGACCAGAAGGGTAAGATTAGGAGAATCGACTGCCTGCGACAGGCAGACAAAGTCT
  GGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGCATCCCCTTGGAAAGTACCGATG
  GAGAGCGGCTCATGAAATCCCCACATTGCACAAACCCAGCACTTTGTGTCCAGCCACATC
  ATATCACAGTATCAGTTAAGGAGCTTGATTTGTTTTTGGCATACTACGTGCAGGAGCAAG
  ATTCTGGACAATCAGGAAGTCCAAGCCACAATGATCCTGCCAAGAATCCTCCAGGTTACC
  TTGAGGATAGTTTTGTAAAATCTGGAGTCTTCAATGTATCAGAACTTGTAAGAGTATCCA
  GAACGCCCATAACCCAGGGAACTGGAGTCAACTTCCCAATTGGAGAAATCCCAAGCCAAC
  CATACTATCATGACATGAACTCGGGGGTCAATCTTCAGAGGTCTCTGTCTTCTCCACCAA
  GCAGCAAAAGACCCAAAACTATATCCATAGATGAAAATATGGAACCAAGTCCTACAGGAG
  ACTTTTACCCCTCTCCAAGTTCACCAGCTGCTGGAAGTCGAACATGGCACGAAAGAGATC
  AAGATATGTCTTCTCCGACTACTATGAAGAAGCCTGAAAAGCCATTGTTCAGCTCTGCAT
  CTCCACAGGATTCTTCCCCAAGACTGAGCACTTTCCCCCAGCACCACCATCCCGGAATAC
  CTGGAGTTGCACACAGTGTCATCTCAACTCGAACTCCACCTCCACCTTCACCGTTGCCAT
  TTCCAACACAAGCTATCCTTCCTCCAGCCCCATCGAGCTACTTTTCTCATCCAACAATCA
  GATATCCTCCCCACCTGAATCCTCAGGATACTCTGAAGAACTATGTACCTTCTTATGACC
  CATCCAGTCCACAAACCAGCCAGTCCTGGTACCTGGGCTAGCTTGGTTCCTTTCCAAGTG
  TCAAATAGGACACCCATCTTACCGGCCAATGTCCAAAATTACGGTTTGAACATAATTGGA
  GAACCTTTCCTTCAAGCAGAAACAAGCAACTGAGGGAAAAAGAAACACAACAATAGTTTA
  AGAAATTTTTTTTTTAAATAAAAAAAAAGGAAAAGAGGAAGACTGGACAAAACAACACAA
  AGGCAGAAAGGAAAGAAACTGAAGAAAGAAGATAATAGACCAGCAATTGCAGCACTTACA
  ATCACTAATTCCCTTAAGGTTGAAACTGTAATGACATAAAAAGGGTCGATGATATTTCAC
  TGATGGTAGATCGCAGCCCCTGCAACGTAGCCTTTGTTACATGAAGTCCGCTGGGAAATA
  GATGTTCTGTCTCTATGACAATATATTTTAACTGACTTTCTAGATGCCTTAATATTTGCA
  TGATAAGCTAGTTTTATTGGTTTAGTATTCTTGTTGTTTACGCATGGAATCACTATTCCT
  GGTTATCTCACCAACGAAGGCTAGGAGGCGGCGTCAGAGGTGCTGGGTGACAGAGCCATG
  AGCCAGCCATTTTATAAGCACTCTGATTTCTAAAAGTTAAAAAAAATATATGAAATCTCT
  GTAGCCTTTAGTTATCAGTACAGATTTATTAAATTTCGGCCCTTAACCCAGCCTTTTCCA
  GTGTGTAACCCAGTTTGAAATCTTAAAAAAAGAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005596
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005596.2, NP_005587.2
• RefSeq Size: 8159 bp
• RefSeq ORF: 1263 bp
• Locus ID: 4781
• Cytogenetics: 9p23-p22.3
• Domains: CTF_NFI, DWA
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: ''
  Transcript Variant: This variant (3) lacks alternate exons in the 3' end, compared to variant 1. The resulting protein (isoform 3) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.