TEAD4 (NM_201443) Human Untagged Clone

CAT#: SC323802

TEAD4 (untagged)-Human TEA domain family member 4 (TEAD4), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TEAD4
• Synonyms: EFTR-2; hRTEF-1B; RTEF1; TCF13L1; TEF-3; TEF3; TEFR-1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_201443.1 GTCCTCCTCGCACACTCGAGGCCAGGGGGCGGGAGGGCCGCAGCTCCGGCGCCGCCGCGT
  CCCGCCAGAACGATCGCCGCGGCCGGAAGAGTTGGCGCTCGGGGCGGACTCCTTGGAACT
  GGCTTAGCGCACCCATCCCACCTTCCCGCACCCTGGGACCGGTCCAACGAGCGCTCCTCC
  AAGCGGAGCCTTGGAGGGCACGGCCGGCACCATTACCTCCAACGAGTGGAGCTCTCCCAC
  CTCCCCTGAGGGGAGCACCGCCTCTGGGGGCAGTCAGGCACTGGACAAGCCCATCGACAA
  TGACGCAGAGGGCGTGTGGAGCCCGGATATTGAGCAGAGTTTCCAGGAGGCCCTCGCCAT
  CTACCCGCCCTGTGGCAGGCGCAAAATCATCCTGTCGGACGAGGGCAAGATGTATGGTCG
  GAACGAGCTGATTGCCCGCTACATCAAGCTCCGGACAGGGAAGACCCGCACCAGGAAGCA
  GGTCTCCAGCCACATCCAGGTGCTGGCTCGTCGCAAAGCTCGCGAGATCCAGGCCAAGCT
  AAAGGACCAGGCAGCTAAGGACAAGGCCCTGCAGAGCATGGCTGCCATGTCGTCTGCACA
  GATCATCTCCGCCACGGCCTTCCACAGTAGCATGGCCCTCGCCCGGGGCCCCGGCCGCCC
  AGCAGTCTCAGGGTTTTGGCAAGGAGCTTTGCCAGGCCAAGCCGGAACGTCCCATGATGT
  GAAGCCTTTCTCTCAGCAAACCTATGCTGTCCAGCCTCCGCTGCCTCTGCCAGGGTTTGA
  GTCTCCTGCAGGGCCCGCCCCATCGCCCTCTGCGCCCCCGGCACCCCCATGGCAGGGCCG
  CAGCGTGGCCAGCTCCAAGCTCTGGATGTTGGAGTTCTCTGCCTTCCTGGAGCAGCAGCA
  GGACCCGGACACGTACAACAAGCACCTGTTCGTGCACATTGGCCAGTCCAGCCCAAGCTA
  CAGCGACCCCTACCTCGAAGCCGTGGACATCCGCCAAATCTATGACAAATTCCCGGAGAA
  AAAGGGTGGACTCAAGGATCTCTTCGAACGGGGACCCTCCAATGCCTTTTTTCTTGTGAA
  GTTCTGGGCAGACCTCAACACCAACATCGAGGATGAAGGCAGCTCCTTCTATGGGGTCTC
  CAGCCAGTATGAGAGCCCCGAGAACATGATCATCACCTGCTCCACGAAGGTCTGCTCTTT
  CGGCAAGCAGGTGGTGGAGAAAGTTGAGACAGAGTATGCTCGCTATGAGAATGGACACTA
  CTCTTACCGCATCCACCGGTCCCCGCTCTGTGAGTACATGATCAACTTCATCCACAAGCT
  CAAGCACCTCCCTGAGAAGTACATGATGAACAGCGTGCTGGAGAACTTCACCATCCTGCA
  GGTGGTCACCAACAGAGACACACAGGAGACCTTGCTGTGCATTGCCTATGTCTTTGAGGT
  GTCAGCCAGTGAGCACGGGGCTCAGCACCACATCTACAGGCTGGTGAAAGAATGAGAGAC
  TCGGGGAGCAGGGAGGGGGGAAGAGACGTGTGTGCAGGAAACGGGGACGTGGGGAGGGGA
  CCTGCAGGGGCAGCCCCCTGAAGTGCCAAGAGAGCTGAGAGGAGCAGTTGTGACTCTACC
  CAGGAACAAACTGTGCCTGAACCTGAGGTGCCCAACCCCAAATAAACCCAAGATGCTGTG
  TATTTTCAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_201443
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201443.1, NP_958851.1
• RefSeq Size: 1525 bp
• RefSeq ORF: 918 bp
• Locus ID: 7004
• Cytogenetics: 12p13.33
• Protein Families: Transcription Factors
• Gene Summary: 'This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) lacks the exon containing the non-AUG (UUG) translation initiation codon compared to variant 1. It uses an in-frame, downstream AUG, resulting in an isoform (3) with a shorter N-terminus compared to isoform 1.