D Amino Acid Oxidase (DAO) (NM_001917) Human Untagged Clone

CAT#: SC323834

DAO (untagged)-Human D-amino-acid oxidase (DAO)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAO
• Synonyms: DAAO; DAMOX; OXDA
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001917.3 CGGACAGAGGGCTGGAAACAAGACGCTCCAGAATCAGGAGCTTCCCCTCAGGAAATAGCA
  TCCTGTGTCCCCGCACTGCAGTTGTCTGGTCTCTCCAGCAGTTTGGTACTTCCGGCTGCT
  GCAATGCGTGTGGTGGTGATTGGAGCAGGAGTCATCGGGCTGTCCACCGCCCTCTGCATC
  CATGAGCGCTACCACTCAGTCCTGCAGCCACTGGACATAAAGGTCTACGCGGACCGCTTC
  ACCCCACTCACCACCACCGACGTGGCTGCCGGCCTCTGGCAGCCCTACCTTTCTGACCCC
  AACAACCCACAGGAGGCGGACTGGAGCCAACAGACCTTTGACTATCTCCTGAGCCATGTC
  CATTCTCCCAACGCTGAAAACCTGGGCCTGTTCCTAATCTCGGGCTACAACCTCTTCCAT
  GAAGCCATTCCGGACCCTTCCTGGAAGGACACAGTTCTGGGATTTCGGAAGCTGACCCCC
  AGAGAGCTGGATATGTTCCCAGATTACGGCTATGGCTGGTTCCACACAAGCCTAATTCTG
  GAGGGAAAGAACTATCTACAGTGGCTGACTGAAAGGTTAACTGAGAGGGGAGTGAAGTTC
  TTCCAGCGGAAAGTGGAGTCTTTTGAGGAGGTGGCAAGAGAAGGCGCAGACGTGATTGTC
  AACTGCACTGGGGTATGGGCTGGGGCGCTACAACGAGACCCCCTGCTGCAGCCAGGCCGG
  GGGCAGATCATGAAGGTGGACGCCCCTTGGATGAAGCACTTCATTCTCACCCATGACCCA
  GAGAGAGGCATCTACAATTCCCCGTACATCATCCCAGGGACCCAGACAGTTACTCTTGGA
  GGCATCTTCCAGTTGGGAAACTGGAGTGAACTAAACAATATCCAGGACCACAACACCATT
  TGGGAAGGCTGCTGCAGACTGGAGCCCACACTGAAGAATGCAAGAATTATTGGTGAACGA
  ACTGGCTTCCGGCCAGTACGCCCCCAGATTCGGCTAGAAAGAGAACAGCTTCGCACTGGA
  CCTTCAAACACAGAGGTCATCCACAACTATGGCCATGGAGGCTACGGGCTCACCATCCAC
  TGGGGATGTGCCCTGGAGGCAGCCAAGCTCTTTGGGAGAATCCTGGAAGAAAAGAAATTG
  TCCAGAATGCCACCATCCCACCTCTGAAGACTCCAGTGACTGCTGCCTCCCCCCACAAGA
  ACTCCCTTCTCCCCTCAGCCAATGAATCAATGTGCTCCTTCATAAGCCATTGCTTCTCCC
  TCACTTCTTTCCTCAAAGAAGCATGAGGTGAGAGAAAGCCACAAAGTCAGTGCCTGGAGA
  AGGGTTCAGCCCAACATGGGGCCCCTCTCATCACTGAAATCCCTCTACCTTCTCTGGGTC
  TGGCATTATAAAGAACAGCTGAGGCTGTCATTCCATGAGTCTTCAGAAGAAAGGACAGCT
  CAGAAAATCAAAGAGGCCAACTGCCCAGAGCCACAGAAAATGGAGGATAATTGAGGCTAA
  GTAACCTGATTACAAGTTGTACTAACATATTAAAGGTTCTGAAAAGTCCTGCAAAAAAAA
  AAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001917
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001917.3, NP_001908.2
• RefSeq Size: 1576 bp
• RefSeq ORF: 1044 bp
• Locus ID: 1610
• Cytogenetics: 12q24.11
• Domains: DAO
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, D-Arginine and D-ornithine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]'